Pretreatment of Chromobacterium viscosum lipase with acetone increases its activity in sodium bis‐(2‐ethylhexyl) sulfosuccinate (AOT) reverse micelles

Abstract

The activity of Chromobacterium viscosum lipase for hydrolysis of olive oil in sodium bis-(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles was increased by pretreatment with acetone. In contrast to the untreated lipase, no sharp fall in the activity of the treated lipase at higher W 0 (water to AOT molar ratio) values was observed. The fluorescence emission intensity of the treated lipase in reverse micelles was higher than that of the untreated lipase but the maximal emission wavelength (λ max ) was the same for both lipases. A kinetic model that considers the free substrate in equilibrium with the substrate adsorbed on the micellar surface was successfully used to better understand the activity enhancement. The Michaelis constant (K m ) and substrate adsorption equilibrium constant (K ad ) were reduced by lipase pretreatment with acetone whereas the maximum reaction rate (v max ) remained unaltered.

Purification and Characterization of Halophilic Alkaline Lipase from Halobacillus sp.

“…Therefore, this lipase can confirmed as organic solvent tolerant, and can be used for industrial level applications like esterification and transesterification processes. Previously it was reported that pretreatment of Chromobacterium viscosum lipase with acetone improved the affinity of enzyme towards the substrate [26]. In accordance to the present study, Seghal Kiran et al [27] reported the high acetone tolerant lipase produced by Pseudomonas sp.…”

Water‐in‐ionic liquid microemulsion‐based organogels as novel matrices for enzyme immobilization

“…The biocatalytic behavior and the stability of two commercial lipases, namely lipase B from Candida antarctica (CalB) and lipase from Chromobacterium viscosum (Cvl), was investigated and compared to that observed either in w/o MBGs, or in w/IL microemulsions. Lipases used in the present work are widely employed in various biocatalytic processes [8,[17][18][19][20][21][22]. Their catalytic behavior has been reported to differ, resulting from structural differences; Cvl has a well-formed lid and presents interfacial activation, acting as a typical lipase, while CalB has a very small lid and does not exhibit interfacial activation [23][24][25][26][27].…”

Thermostability of Cromobacterium viscosum lipase in AOT/isooctane reverse micelle

“…To improve the activity of lipases entrapped in AOT reverse micelles, several methods have been reported: the use of chemically modified AOT (9), pretreatment with acetone (10), and the introduction of nonionic surfactant such as Tween-85 or small molecular weight polyethylene glycols (PEGs) as additives (11)(12)(13). In addition to the activity of lipase, stability and thermostability are prerequisites for the industrial application of lipase.…”