Pretreatment of corn stover using low-moisture anhydrous ammonia (LMAA) process

Yoo, Chang Geun; Nghiem, Nhuan P.; Hicks, Kevin B.; Kim, Tae Hyun

doi:10.1016/j.biortech.2011.08.057

Cited by 92 publications

(51 citation statements)

References 24 publications

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“…The LMAA pretreatment was performed by modifying the Kim method [15]. Water (100 g) was added dropwise to the dry powdered napiergrass (100 g, volume 320 mL) in the flask (1 L).…”

Section: Lmaa Pretreatmentmentioning

confidence: 99%

“…In many cases, the PF using recombinant E. coli [22][23][24] or recombinant S. cerevisiae [25] has been incorporated with the process as cofermentation with hexose fermentation [15,26]. However, we performed the PF step separately, since HF using S. cerevisiae was well-established technique.…”

Section: Strategy For Ethanol Production From Napiergrassmentioning

confidence: 99%

“…Also, soaking in aqueous ammonia (SAA) at 40˚C -80˚C in a room atmosphere was used as the pretreatment of lignocellulose [13,14]. Recently, Kim et al [15] developed a pretreatment using gaseous ammonia, low-moisture anhydrous ammonia (LMAA) pretreatment, where the lingocellulose was kept in a flask filled with ammonia gas at 80˚C for 84 h.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Yasuda¹,

Takeo²,

Nagai³

et al. 2013

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Napiegrass (Pennisetum purpureum Schumach) was treated with a low-moisture anhydrous ammonia (LMAA) pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation (SSF) was conducted for the LMAA-pretreated napiergrass (1.33 g) in a buffer solution (8 mL) using a mixture of a cellulase (80 mg) and a xylanase (53 mg) as well as the cell suspension (0.16 mL) of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass (100.0 g) to give ethanol (77.2%) and xylose (52.8%). After the removal of ethanol, the pentose fermentation of the SSF solution (40 mL), which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 (70 mL) gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan (21.4 wt%) and glucan (39.7 wt%) of the LMAA-pretreated napiergrass.

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“…The LMAA pretreatment was performed by modifying the Kim method [15]. Water (100 g) was added dropwise to the dry powdered napiergrass (100 g, volume 320 mL) in the flask (1 L).…”

Section: Lmaa Pretreatmentmentioning

confidence: 99%

Section: Strategy For Ethanol Production From Napiergrassmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Yasuda¹,

Takeo²,

Nagai³

et al. 2013

JSBS

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“…This operation was performed three times until the atmosphere inside the flask was entirely replaced with gaseous ammonia. The LMAA pretreatment was performed by modifying the Kim method where LMAA pretreatment was performed at 80˚C for 86 h [13]. In our LMAA-pretreatment [14], the moist powdered lignocellulose was kept under NH 3 gas atmosphere at room temperature for four weeks.…”

Section: Pretreatment Of Lignocellulosementioning

confidence: 99%

Fuelization of Italian Ryegrass and Napier Grass through a Biological Treatment and Photocatalytic Reforming

Yasuda¹,

nbsp²,

Takenouchi³

et al. 2015

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Fuelization of Italian ryegrass and Napier grass was examined by the combination of biological treatments and photocatalytic reforming (photo-Reform). The alkali-pretreated Italian ryegrass and Napier grass were subjected to the enzymatic saccharification using cellulase and xylanase. Xylose and glucose were produced in 56.6% and 71.1% from Italian ryegrass and in 49.5% and 67.3% from Napier grass, respectively. Xylose and glucose were converted to hydrogen by the photo-Reform using a Pt-loaded titanium oxide (Pt/TiO2) under UV irradiation. Moreover, a lowmoisture anhydrous ammonia (LMAA) pretreatment was performed for Italian ryegrass and Napier grass by keeping moist powdered biomass under NH3 gas atmosphere at room temperature for four weeks. The Italian ryegrass and Napier grass which were pretreated by LMAA method were subjected to simultaneous saccharification and fermentation (SSF) using a mixture of cellulase and xylanase as well as Saccharomyces cerevisiae in acetate buffer (pH 5.0). Ethanol and xylose were produced in 91.6% and 51.6% from LMAA-pretreated Italian ryegrass and 78.6% and 68.8% from Napier grass, respectively. After the evaporation of ethanol, xylose was converted to hydrogen by the photo-Reform. In the case of saccharification→photo-Reform, energy was recovered as hydrogen from the alkali-pretreated Italian ryegrass and Napier grass in 71.9% and 77.0% of energy recovery efficiency, respectively. In the case of SSF→photo-Reform, the energy was recovered in 82.7% and 77.2% as ethanol and hydrogen from the LMAA-pretreated Italian ryegrass and Napier grass, respectively.

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“…What's more, various studies focused on pretreatment methods have been conducted to enhance enzymatic digestibility and improve ethanol yield based on corn stover. Among all chemical reagents used for pretreatment, ammonia stands out because of its delignification, high preservation of glucose and swelling effects (Yoo, et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Optimization of Low-Moisture Anhydrous Ammonia (LMAA) Pretreatment of Corn Stover

Yang¹,

Rosentrater²

2014

2014 ASABE Annual International Meeting

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With many environmental benefits, corn-based ethanol has been widely used in recent years. Cellulosic ethanol, however, will require pretreatment to break down lingo-cellulosic structures prior to fermentation. Among all the pretreatment reagents which can be used, ammonia has been shown to be one of the most effective, because it can readily delignify, swell, and preserve the cellulose. Previous work evaluated the effectiveness of the LMAA pretreatment method. The purpose of this study was to determine optimal conditions (i.e. highest glucose yield) using the LMAA pretreatment process. In this experiment, corn stover was prepared with different moisture contents (20%, 50%, 80% wt) and particle sizes (9-30 mesh, 30-144 mesh). Corn stover was ammoniated at 20 psi for 30 minutes. Ammoniated corn stover then was subjected to different incubation times (24h, 75h, and 144h) under different temperatures (20C, 70C, 120C). After that, compositional analyses, including ash content, solids content, structural carbohydrates, and lignin content, were conducted. Enzymatic digestibility tests were also conducted. Results from this study will be used to improve the efficiency of pretreatment processing. Keywords LMAA, Ammonia, Ethanol Disciplines Agriculture | Bioresource and Agricultural EngineeringThis conference proceeding is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/abe_eng_conf/390The authors are solely responsible for the content of this technical presentation. The technical presentation does not necessarily reflect the official position of the American Society of Agricultural and Biological Engineers (ASABE), and its printing and distribution does not constitute an endorsement of views which may be expressed. Technical presentations are not subject to the formal peer review process by ASABE editorial committees; therefore, they are not to be presented as refereed publications. Citation of this work should state that it is from an ASABE meeting paper. EXAMPLE: Author's Last Name, Initials.

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Pretreatment of corn stover using low-moisture anhydrous ammonia (LMAA) process

Cited by 92 publications

References 24 publications

Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Fuelization of Italian Ryegrass and Napier Grass through a Biological Treatment and Photocatalytic Reforming

Optimization of Low-Moisture Anhydrous Ammonia (LMAA) Pretreatment of Corn Stover

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Pretreatment of corn stover using low-moisture anhydrous ammonia (LMAA) process

Cited by 92 publications

References 24 publications

Enhancement of Ethanol Production from Napiergrass (&lt;i&gt;Pennisetum purpureum&lt;/i&gt; Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Enhancement of Ethanol Production from Napiergrass (&lt;i&gt;Pennisetum purpureum&lt;/i&gt; Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Fuelization of Italian Ryegrass and Napier Grass through a Biological Treatment and Photocatalytic Reforming

Optimization of Low-Moisture Anhydrous Ammonia (LMAA) Pretreatment of Corn Stover

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Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment