Presumptive TRP channel CED-11 promotes cell volume decrease and facilitates degradation of apoptotic cells in
            <i>Caenorhabditis elegans</i>

Driscoll, Kaitlin; Stanfield, Gillian M.; Droste, Rita; Horvitz, H. Robert

doi:10.1073/pnas.1705084114

Cited by 10 publications

(5 citation statements)

References 38 publications

(43 reference statements)

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“…It has been previously noted that several species express TRPM-like channels which cluster independent of, or differ greatly from, other chordate and/or insect TRPMs [28,52,81]. In accordance with this, the consensus TRPM phylogeny shows a group of protostome TRPMs located basally, near choanoflagellate TRPMs (figure 5b, green).…”

Section: (C) Residues Critical To Menthol Sensing Were Probably Present In Ancestral Bilaterian Channelssupporting

confidence: 77%

Drosophila menthol sensitivity and the Precambrian origins of transient receptor potential-dependent chemosensation

Himmel

Letcher

Sakurai

et al. 2019

Phil. Trans. R. Soc. B

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Transient receptor potential (TRP) cation channels are highly conserved, polymodal sensors which respond to a wide variety of stimuli. Perhaps most notably, TRP channels serve critical functions in nociception and pain. A growing body of evidence suggests that transient receptor potential melastatin (TRPM) and transient receptor potential ankyrin (TRPA) thermal and electrophile sensitivities predate the protostome–deuterostome split (greater than 550 Ma). However, TRPM and TRPA channels are also thought to detect modified terpenes (e.g. menthol). Although terpenoids like menthol are thought to be aversive and/or harmful to insects, mechanistic sensitivity studies have been largely restricted to chordates. Furthermore, it is unknown if TRP-menthol sensing is as ancient as thermal and/or electrophile sensitivity. Combining genetic, optical, electrophysiological, behavioural and phylogenetic approaches, we tested the hypothesis that insect TRP channels play a conserved role in menthol sensing. We found that topical application of menthol to Drosophila melanogaster larvae elicits a Trpm - and TrpA1 -dependent nocifensive rolling behaviour, which requires activation of Class IV nociceptor neurons. Further, in characterizing the evolution of TRP channels, we put forth the hypotheses that three previously undescribed TRPM channel clades (basal, αTRPM and βTRPM), as well as TRPs with residues critical for menthol sensing, were present in ancestral bilaterians. This article is part of the Theo Murphy meeting issue ‘Evolution of mechanisms and behaviour important for pain’.

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Section: (C) Residues Critical To Menthol Sensing Were Probably Present In Ancestral Bilaterian Channelssupporting

confidence: 77%

Drosophila menthol sensitivity and the Precambrian origins of transient receptor potential-dependent chemosensation

Himmel

Letcher

Sakurai

et al. 2019

Phil. Trans. R. Soc. B

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“…The function of TRPS, however, remains mostly mysterious. Only a single member has been functionally characterized: C. elegans ced-11 , which encodes a Ca 2+ channel which participates in apoptosis [110].…”

Section: Trp Familiesmentioning

confidence: 99%

Transient receptor potential channels: current perspectives on evolution, structure, function and nomenclature

Himmel

Cox

2020

Proc. R. Soc. B.

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The transient receptor potential superfamily of ion channels (TRP channels) is widely recognized for the roles its members play in sensory nervous systems. However, the incredible diversity within the TRP superfamily, and the wide range of sensory capacities found therein, has also allowed TRP channels to function beyond sensing an organism's external environment, and TRP channels have thus become broadly critical to (at least) animal life. TRP channels were originally discovered in Drosophila and have since been broadly studied in animals; however, thanks to a boom in genomic and transcriptomic data, we now know that TRP channels are present in the genomes of a variety of creatures, including green algae, fungi, choanoflagellates and a number of other eukaryotes. As a result, the organization of the TRP superfamily has changed radically from its original description. Moreover, modern comprehensive phylogenetic analyses have brought to light the vertebrate-centricity of much of the TRP literature; much of the nomenclature has been grounded in vertebrate TRP subfamilies, resulting in a glossing over of TRP channels in other taxa. Here, we provide a comprehensive review of the function, structure and evolutionary history of TRP channels, and put forth a more complete set of non-vertebrate-centric TRP family, subfamily and other subgroup nomenclature.

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“…This decrease is likely induced by the opening of potassium and chloride channels in the plasma membrane, causing an efflux of potassium and chloride ions followed by water [ 4 ]. Blocking this shrinkage in apoptotic cells in Caenorhabditis elegans embryos compromises their ability to die, which suggests that cell shrinkage facilitates the execution of apoptosis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

A caspase–RhoGEF axis contributes to the cell size threshold for apoptotic death in developing Caenorhabditis elegans

et al. 2022

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A cell’s size affects the likelihood that it will die. But how is cell size controlled in this context and how does cell size impact commitment to the cell death fate? We present evidence that the caspase CED-3 interacts with the RhoGEF ECT-2 in Caenorhabditis elegans neuroblasts that generate “unwanted” cells. We propose that this interaction promotes polar actomyosin contractility, which leads to unequal neuroblast division and the generation of a daughter cell that is below the critical “lethal” size threshold. Furthermore, we find that hyperactivation of ECT-2 RhoGEF reduces the sizes of unwanted cells. Importantly, this suppresses the “cell death abnormal” phenotype caused by the partial loss of ced-3 caspase and therefore increases the likelihood that unwanted cells die. A putative null mutation of ced-3 caspase, however, is not suppressed, which indicates that cell size affects CED-3 caspase activation and/or activity. Therefore, we have uncovered novel sequential and reciprocal interactions between the apoptosis pathway and cell size that impact a cell’s commitment to the cell death fate.

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Presumptive TRP channel CED-11 promotes cell volume decrease and facilitates degradation of apoptotic cells in Caenorhabditis elegans

Cited by 10 publications

References 38 publications

Drosophila menthol sensitivity and the Precambrian origins of transient receptor potential-dependent chemosensation

Drosophila menthol sensitivity and the Precambrian origins of transient receptor potential-dependent chemosensation

Transient receptor potential channels: current perspectives on evolution, structure, function and nomenclature

A caspase–RhoGEF axis contributes to the cell size threshold for apoptotic death in developing Caenorhabditis elegans

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