Presumptive involvement of methyl ricinoleate β-oxidation in the production of γ-decalactone by the yeastPichia guilliermondii

Abstract

Production of γ‐decalactone by yeasts from fatty acids has been reported but little is known about the mechanisms involved in this process. This paper provides information about the mechanisms involved in the production of γ‐decalactone by Pichia guilliermondii in the presence of a fatty acid methyl ester. Culturing of P. guilliermondii in media containing methyl ricinoleate (12(R)‐hydroxy‐9(Z)‐octadecenoic acid) revealed a coordinated induction of β‐oxidation activities and γ‐decalactone production. However, no γ‐decalactone synthesis was noted when methyl ricinoleate was changed into methyloleate or methyl linoleate, even though these fatty acid methyl esters are able to induce β‐oxidation activities in P. guilliermondii. These observations led us to conclude that methyl ricinoleate is an inducer of β‐oxidation and is probably the substrate for γ‐decalactone production. The fatty acid ester β‐oxidation should be involved, at least in part, in this production.

Production of γ-Decalactone by a Psychrophilic and a Mesophilic Strain of the Yeast Rhodotorula aurantiaca

“…The production was reduced for higher castor oil contents (30, 40, 50, and 60 g/l). Similar results have been reported by Endrizzi et al [21], who observed that a high concentration of ricinoleic acid methyl ester lowers the yield of γ-decalactone produced by Pichia guilliermondii. However, our results contrasted with those reported by Dufossé et al [15], who showed that γ-decalactone concentration obtained in the culture broth of Sporidiobolus ruinenii increased almost proportionally with a ricinoleic acid methyl ester concentration between 0 and 160 g/l.…”

“…Indeed, peroxysomal β-oxidation enzymes were shown to be responsible for γ-decalactone production in other yeast strains [7,21,[27][28][29].…”

Generation of Flavors and Fragrances Through Biotransformation and De Novo Synthesis

“…In these studies, GDL reconsumption was observed due to Aox3 activity, which led to an increased 3-OH-GDL production. GDL reconsumption has been reported as an issue in older studies (Aguedo et al 2005;Endrizzi et al 1993;Waché et al 2001). Braga et al (2015a) compared lactone production profiles by Y. lipolytica W29 and two W29 mutants, MTLY40-2P (POX3-5 disrupted and POX2 overexpressed) and JMY3010.…”