Presumed Paternal Genome Loss During Embryogenesis of Predatory Phytoseiid Mites

Toyoshima, Satoshi; Amano, Hiroshi

doi:10.5772/37477

Cited by 4 publications

(9 citation statements)

References 45 publications

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“…Phytoseiid mites have a specific reproduction pattern called paternal genome elimination, and it is still wrapped in mystery, although cytological and genetic evidence has accumulated since the 1980s 29 . Maternal control of offspring sex was demonstrated through sex ratio control under certain nutrition conditions 29 . However, how female mites regulate the sex of their offspring under different nutritional status is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Neoseiulus californicus , found in various crops in temperate and subtropical regions, is a predatory mite commercially used as a biological control agent against phytophagous mites including the two‐spotted mite ( Tetranychus urticae ) and some small insects such as thrips 25–28 . The mode of reproduction in most phytoseiid mites is termed pseudo‐arrhenotoky 29 . The adult female begins oviposition only after copulation, and both males and females begin embryonic development as diploids.…”

Section: Introductionmentioning

confidence: 99%

“…The adult female begins oviposition only after copulation, and both males and females begin embryonic development as diploids. The paternal chromosomes are eliminated during embryogenesis in males, and they develop into functionally haploid adults 29 . The elimination of the paternal chromosomes in phytoseiid mites is a mystery, and little is known about the sex‐determination mechanisms at the gene level.…”

Section: Introductionmentioning

confidence: 99%

“…[25][26][27][28] The mode of reproduction in most phytoseiid mites is termed pseudo-arrhenotoky. 29 The adult female begins oviposition only after copulation, and both males and females begin embryonic development as diploids. The paternal chromosomes are eliminated during embryogenesis in males, and they develop into functionally haploid adults.…”

Section: Introductionmentioning

confidence: 99%

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Identification of potential sex determination genes and functional analyses in Neoseiulus californicus under prey stress

Zhu

Guo

et al. 2022

Pest Management Science

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BACKGROUND Phytoseiid mites are important natural enemies of spider mites. Sex‐determination mechanism are important basic scientific issues in the reproduction and evolution of predatory mites. Clarifying sex‐determination mechanism may provide reference for exploring genetic approach to have the phytoseiid mites produce more female offspring, which could improve their effectiveness as a biological control agent. RESULTS We used transcriptome sequencing to identify and characterize 20 putative sex‐determination genes in the phytoseiid mite Neoseiulus californicus, a species with uncommon pseudo‐arrhenotoky, including doublesex‐like (dsx1‐like), transformer‐2 (tra‐2), intersex (ix), and fruitless‐like (BTB2). A significant negative correlation was found between prey stress and offspring sex ratio. But the most genes identified showed no difference in expression between the groups with lowest and highest female offspring ratios. The hatching rate and sex ratio of female offspring were reduced when the ix gene was silenced, and the oviposition days and fecundity were reduced when the BTB2 gene was silenced. The fecundity was reduced when the tra2 gene was silenced and the snf gene is essential for oviposition in female. There was no effect on reproduction and female sex determination when silencing the dsx1‐like and dsx2‐like gene. CONCLUSION The genes BTB2, tra2 and snf are involved in oviposition, and ix may be involved in female sex determination and egg formation in Neoseiulus californicus. The results are conductive to further understanding molecular regulatory mechanism of sex determination in predatory mites and may provide a reference for better use of this predatory by producing more females. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of potential sex determination genes and functional analyses in Neoseiulus californicus under prey stress

Zhu

Guo

et al. 2022

Pest Management Science

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“…Amano, 2012). Neste tipo de reprodução, apesar de machos e fêmeas serem gerados por ovos fecundados, os ovos que dão origem aos machos têm seus cromossomos paternos inativados tornando-se assim haploides.…”

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Seleção de linhagem de >i<Amblyseius tamatavensis>/i< (Acari: Phytoseiidae) mais eficiente no controle de >i<Bemisia tabaci>/i< Biotipo B

Silva¹

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pela atenção com que sempre me orientou, aconselhou e ensinou, permitindo que este trabalho fosse realizado. Agradeço também pela confiança depositada em mim quando me deu a oportunidade de trabalhar ao seu lado. Ao Prof. Dr. Antônio A. D. Coelho, por aceitar ser meu coorientador e pelos ensinamentos que possibilitaram a realização deste trabalho. Ao Prof. Dr. Alberto Soares Correa pela imensa ajuda com a parte molecular e a todos do Laboratório de Ecologia Molecular de Artrópodes por sempre me ajudarem tão gentilmente. Ao Prof. Dr. Carlos H. W. Flechtmann pelo auxílio prestado na solução de dúvidas. Aos meus pais, José Roberto R. da Silva e Silvana M. R. da Silva, e minha irmã Gabriela M. R. da Silva, pelo apoio e por sempre acreditarem em mim; sem eles nada disso seria possível. Ao meu companheiro, Guilherme A. Augusto, pelo incentivo e apoio nos momentos difíceis, por nunca me deixar desistir de meus objetivos. Aos meus amigos e colegas de laboratório com quem pude conviver durante todos esses anos, em especial à Marielle M. Berto, Fernanda N. Esteca e Mariana Yamada por me alegrarem nos momentos difíceis. À Me. Michele Ennes pela amizade e pelo auxílio na obtenção dos indivíduos de A. tamatavensis do município de Mogi-guaçu (São Paulo). Ao Dr. Geovanny S. P. Barroso pela amizade e pelo auxílio com a obtenção dos indivíduos de A. tamatavensis coletados em Olho d'Água das Flores (Alagoas). À Dra. Diana M. Rueda, pela amizade e por gentilmente sempre me ajudar com as dúvidas em estatística. À Me. Sofia, pela amizade e pelo auxílio na obtenção dos indivíduos de A. tamatavensis coletados no Peru. À Dra. Marina F. C. Barbosa pela amizade, ensinamentos e conselhos. Ao Sr. Luiz Kumagai, por autorizar a coleta dos indivíduos de A. tamatavensis em sua fazenda, localizada na cidade de Campinas (São Paulo).

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Expression analysis of Drosophila doublesex, transformer-2, intersex, fruitless-like, and vitellogenin homologs in the parahaploid predator Metaseiulus occidentalis (Chelicerata: Acari: Phytoseiidae)

Pomerantz

Hoy

2014

Exp Appl Acarol

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Characterization and expression analyses are essential to gain insight into sex-determination pathways in members of the Acari. Little is known about sex determination at the molecular level in the western orchard predatory mite Metaseiulus occidentalis (Arthropoda: Chelicerata: Arachnida: Acari: Phytoseiidae), a parahaploid species. In this study, eight genes previously identified as putative homologs to genes involved in the sex-determination pathway in Drosophila melanogaster were evaluated for sex-specific alternative splicing and sex-biased expression using reverse-transcriptase PCR and quantitative real-time PCR techniques, respectively. The homologs evaluated in M. occidentalis included two doublesex-like genes (Moccdsx1 and Moccdsx2), transformer-2 (Mocctra-2), intersex (Moccix), two fruitless-like genes (MoccBTB1 and MoccBTB2), as well as two vitellogenin-like genes (Moccvg1 and Moccvg2). Single transcripts of equal size were detected in males and females for Moccdsx1, Moccdsx2, Mocctra-2, Moccix, and MoccBTB2, suggesting that their pre-mRNAs do not undergo alternative splicing in a sex-specific manner. Three genes, Moccdsx1, Moccdsx2 and MoccBTB2, displayed male-biased expression relative to females. One gene, Moccix, displayed female-biased expression relative to males. Two genes, Mocctra-2 and MoccBTB1, did not display detectable differences in transcript abundance in males and females. Expression of Moccvg1 and Moccvg2 were detected in females only, and transcript levels were up-regulated in mated females relative to unmated females. To our knowledge, this represents the first attempt to elucidate expression patterns of putative sex-determination genes in an acarine. This study is an initial step towards understanding the sex-determination pathway in the parahaploid M. occidentalis.

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Presumed Paternal Genome Loss During Embryogenesis of Predatory Phytoseiid Mites

Cited by 4 publications

References 45 publications

Identification of potential sex determination genes and functional analyses in Neoseiulus californicus under prey stress

Identification of potential sex determination genes and functional analyses in Neoseiulus californicus under prey stress

Seleção de linhagem de >i<Amblyseius tamatavensis>/i< (Acari: Phytoseiidae) mais eficiente no controle de >i<Bemisia tabaci>/i< Biotipo B

Expression analysis of Drosophila doublesex, transformer-2, intersex, fruitless-like, and vitellogenin homologs in the parahaploid predator Metaseiulus occidentalis (Chelicerata: Acari: Phytoseiidae)

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