Presumed monozygotic twins develop following transfer of an &lt;i&gt;in vitro&lt;/i&gt;-produced equine embryo

Roberts, Melissa A.; London, Kelly; Campos-Chillon, L. F.; Altermatt, J. L.

doi:10.1294/jes.26.89

Cited by 8 publications

(11 citation statements)

References 15 publications

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“…We recently reported that a small embryonic vesicle on day 7 after transfer of an IVP embryo was associated with an increased incidence of early embryonic loss . Similar to our findings, all cases of multiple pregnancy after transfer of single IVP (n = 1) and in vivo (n = 6) equine embryo in other reports resulted in either early pregnancy loss or late‐term abortion . Since twin reduction was only attempted in one set of twins, we can only speculate on the likely success of twin reduction.…”

Section: Discussionsupporting

confidence: 89%

“…Roberts et al . described series of ultrasound examinations of a twin pregnancy that developed after ICSI that they presumed to be monozygotic. This report expands upon the possibility of monozygotic multiple pregnancy after IVEP by describing the incidence and characteristics of monozygotic twins and triplets after transfer of single frozen‐thawed IVP equine embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Notwithstanding the potential advantages of IVEP, it is reasonable to suggest that IVEP via ICSI and in vitro culture and/or the cryopreservation process could have some negative consequences. Indeed, there is one report of a single IVP embryo developing into presumed monozygotic twins . In this respect, assisted reproductive technologies (ART) in women are known to increase the incidence of monozygotic twins , which are often referred to as ‘identical’ because they arise from a single embryo which divides sometime during early development.…”

Section: Introductionmentioning

confidence: 99%

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Monozygotic multiple pregnancies after transfer of single in vitro produced equine embryos

Dijkstra

Cuervo‐Arango

Stout

et al. 2019

Equine Veterinary Journal

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Summary Background Monozygotic multiple pregnancy is rare in horses, but may be more common after transfer of an in vitro produced (IVP) embryo. Objectives To determine the occurrence, incidence, characteristics and outcome of monozygotic siblings arising from in vivo and IVP equine embryos. Study design Retrospective case series. Methods A total of 496 fresh in vivo and 410 frozen‐thawed IVP blastocysts, produced by intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes from Warmblood mares, were transferred into recipient mares. The likelihoods of pregnancy and multiple pregnancy were calculated, and the clinical features and outcome of any multiple pregnancy were recorded. Results The likelihood of pregnancy after transfer of a single IVP or in vivo embryo was 62% (254/410) and 83% (413/496) respectively. The incidence of multiple pregnancy was 1.6% (4/254) and 0% (0/413) for IVP and in vivo blastocysts, respectively. More specifically, three IVP blastocysts yielded twin embryo propers/fetuses, and one IVP conceptus developed three distinct embryonic bodies. Interestingly, only one embryonic vesicle was detected at all ultrasonographic examinations prior to embryo proper development. Multiple embryonic bodies only became apparent at later scans to check for an embryo proper and heartbeat, or when the recipient mare aborted. Two twin pregnancies aborted spontaneously at 3 and 9 months, respectively, while the heartbeat was lost from all three embryos in the triplet pregnancy before day 35 of gestation. Twin reduction by per rectum compression of one fetal thorax was attempted at day 50 of gestation in the fourth case; however, both fetuses were lost. Main limitations Small number of cases. Conclusions In vitro embryo production resulted in a higher incidence of multiple monozygotic pregnancy, which could only be diagnosed after development of the embryo proper and is likely to result in pregnancy loss later in gestation if left untreated.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monozygotic multiple pregnancies after transfer of single in vitro produced equine embryos

Dijkstra

Cuervo‐Arango

Stout

et al. 2019

Equine Veterinary Journal

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“…Monozygotic twins and triplets are reported but are considered very rare (Meadows and others 1995, Govaere and others 2009). Proportionately, more cases have been described after embryo transfer (McCue and others 1998, Sper and others 2012, Roberts and others 2015), which may suggest an association between assisted reproductive techniques and monozygous twins, or it may simply be a factor that they are more appropriately recognised after transfer of a single embryo. Newcombe (2000) reported a review of pregnancy records for over 6000 broodmares over seven years and this suggested that up to 2.6 per cent of twin pregnancies may originate from a single ovulatory follicle.…”

Section: The Origin Of Twinsmentioning

confidence: 99%

Management of twins in horses

Crabtree¹

2018

In Practice

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Twin pregnancy is a commonly encountered condition in equine reproductive practice which can have devastating effects for the client in respect to the health and future fertility of their mare as well as significant financial implications. In well-managed situations, twins can be dealt with relatively easily and effectively during early pregnancy. However, due to the nature of clinical practice and the differing stages at which a mare may be presented for examination, the management of twins can be difficult. Complicating factors include twins of differing ages (asynchronous twins), adjacent twins (unilateral), which can be difficult to identify as two separate entities, and the presence of one or multiple endometrial cysts. This article reviews the options available in the management of twins and the likely expected success rate.

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“…In humans, MZ twins are born in 0.3 % of all spontaneous livebirths (Hall, 2003). After in vitro procedure and embryo transfer, the emergence of MZ twins is observed more frequently, as shown in species such as horse (Mancill et al, 2011;Roberts et al, 2015), cattle (Kraay et al, 1983;Niemann and Sacher, 1984) and human (Aston et al, 2008;Blickstein et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Multiplying embryos: experimental monozygotic polyembryony in mammals and its uses

Casser

Israel²,

Boiani³

2019

Int. J. Dev. Biol.

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Monozygotic (MZ) polyembryony is a strategy to increase the output of a single zygote, thereby producing more offspring from a limited number of oocytes. However, MZ twins and multiples (multiplets) of mammals occur rarely in nature, while their generation has been more successful experimentally. In this work, we review some of the methodological, biological and field aspects of experimental MZ polyembryony in mammals. First attempts of mechanical bisection of 2-cell stage rodent embryos provided a proof-of-principle for the survival and independent development of both blastomeres. Subsequently, experiments in other species, particularly sheep and bovine, allowed 2 methods of embryo multiplication to become routine: the separation or biopsy of blastomeres from cleavage-stage embryos and the bisection of morulae and blastocysts. We discuss how the preferable stage of bisection and the success rate can be species-specific. The scope that profited most from experimental MZ polyembryony is the production of additional copies of elite livestock individuals, the reduction of interindividual variation in test groups and the possibility of investigating discordant phenotypic traits in the same genomic background, for instance, comparing an affected twin with its healthy co-twin. By contrast, the original motivation for experimental polyembryony – efficiently generating more offspring out of the same zygote – has not been fulfilled yet. Although embryo splitting leads to an increase in quantity, there is a loss of embryo quality, thus, there is no real gain from artificially generated embryos (yet) in the field of medically assisted reproduction. In conclusion, mammalian zygotes have the regulative capacity to be polyembryonic, but this is not obligate.

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Presumed monozygotic twins develop following transfer of an <i>in vitro</i>-produced equine embryo

Cited by 8 publications

References 15 publications

Monozygotic multiple pregnancies after transfer of single in vitro produced equine embryos

Monozygotic multiple pregnancies after transfer of single in vitro produced equine embryos

Management of twins in horses

Multiplying embryos: experimental monozygotic polyembryony in mammals and its uses

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