Prestin amplifies cardiac motor functions

Zhang, Xiaodong; Thai, Phung N.; Ren, Lu; Flores, Maria Cristina Perez; Ledford, Hannah A.; Park, Seojin; Lee, Jeong Han; Sihn, Choong Ryoul; Chang, Chih Ching; Chen, Wei Chun; Timofeyev, Valeriy; Zuo, Jian; Chan, James W.; Yamoah, Ebenezer N.; Chiamvimonvat, Nipavan

doi:10.1016/j.celrep.2021.109097

Cited by 20 publications

(26 citation statements)

References 48 publications

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“… (B) Representative traces of capacitive currents recorded from WT and Slc26a5 −/− cardiomyocytes at baseline (left panels), after application of 25 μM blebbistatin (BLEB, middle panels) and 10 mM BDM (right panels). Reprinted with permission from ( Zhang et al., 2021 ). (C) Normalized NLC as a function of voltage.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…The NLC of Slc26a5 −/− myocytes at -20, -10, 0, 10, 20, 30, and 40 mV in the presence of BLEB or BDM is significantly smaller than that in the absence of BLEB and BDM (p<0.05). Reprinted with permission from ( Zhang et al., 2021 ). Data represent mean ± SEM.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…However, prestin was recently identified to be functionally expressed in cardiomyocytes to amplify actin-myosin force generation accounting partly for the NLC of cardiomyocytes. Therefore, prestin serves a broader cellular motor function ( Zhang et al., 2021 ). The function of prestin can be directly assessed by NLC recordings and analyses.…”

Section: Before You Beginmentioning

confidence: 99%

“…Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021) .…”

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confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021) .…”

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confidence: 99%

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Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

et al. 2021

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Summary Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021) .

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021) .…”

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confidence: 99%

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Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

et al. 2021

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Prestin in Human Perilymph, Cerebrospinal Fluid, and Blood as a Biomarker for Hearing Loss

Gadenstaetter,

Krumpoeck,

Auinger

et al. 2024

Otolaryngol.--head neck surg.

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ObjectiveDetermining the concentration of prestin in human blood, cerebrospinal fluid (CSF), and perilymph (PL), and evaluating its suitability as a clinical biomarker for sensori‐neural hearing loss (SNHL).Study DesignHuman blood, CSF, and PL samples were intraoperatively collected from 42 patients with tumors of the internal auditory canal or with intracochlear tumors undergoing translabyrinthine or middle fossa tumor removal. Prestin concentration was measured using enzyme‐linked immunosorbent assay and linear regression analyses were performed to investigate its associations with audiological as well as vestibular test results.SettingTertiary referral center.ResultsThe median prestin concentration in blood samples of the 42 study participants (26 women, mean ± standard deviation age, 52.7 ± 12.5 years) was 1.32 (interquartile range, IQR, 0.71‐1.99) ng/mL. CSF prestin levels were significantly higher with 4.73 (IQR, 2.45‐14.03) ng/mL (P = .005). With 84.74 (IQR, 38.95‐122.00) ng/mL, PL prestin concentration was significantly higher compared to blood (P = .01) and CSF (P = .03) levels. Linear regression analyses showed significant associations of CSF prestin concentration with preoperative hearing levels (pure‐tone average and word recognition; P = .008, R2 = 0.1894; P = .03, R2 = 0.1857), but no correlations with blood or PL levels.Conclusion and RelevanceThis study's findings highlight the volatile nature of prestin levels and provide the first insights into this potential biomarker's concentrations in body fluids apart from blood. Future investigations should comprehensively assess human prestin levels with different etiologies of SNHL, prestin's natural homeostasis and systemic circulation, and its temporal dynamics after cochlear trauma. Finally, clinically approved detection kits for prestin are urgently required prior to considering a potential translational implementation of this diagnostic technique.

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SLC26 Anion Transporters

Geertsma,

Oliver

2023

Anion Channels and Transporters

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Prestin amplifies cardiac motor functions

Cited by 20 publications

References 48 publications

Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Prestin in Human Perilymph, Cerebrospinal Fluid, and Blood as a Biomarker for Hearing Loss

SLC26 Anion Transporters

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