Pressurized liquid extraction of cyclitols and sugars: optimization of extraction parameters and selective separation

Abstract

Cyclitols and sugars were obtained as a mixture from Medicago sativa L., in a comparative study by using maceration, and pressurized liquid extraction, as a modern and green extraction techniques. The influence of extraction parameters including: extraction temperature, time and number of cycles on the content of sugars and cyclitols was investigated based on response surface methodology. The highest total amount of sugars and cyclitols (62.27 ± 2.30 and 50.35 ± 0.77 mg/g of dry material, respectively) was obtained when extraction was performed at 88°C, for 22 min, in two cycles. The methodology used involved extraction, purification, selective separation (using yeast and anion exchange resin) and derivatization, followed by gas chromatography ‐mass spectrometry analysis. The use of yeast treatment realized an effective fractionation of cyclitols and sugars, which allowed the removal of most sugars. The involvement of anion exchange resin after yeast allowed the removal of sugar alcohols and lactose, together with other sugar traces remained and to obtain a solution containing six cyclitols. The recrystallization of dry residue after solvent evaporation, from ethanol, allowed us to obtain 14.65 mg of white pure crystals identified with NMR spectroscopy, liquid chromatography with mass spectrometry, gas chromatography with mass spectrometry, optical rotation and melting point as analysis D‐pinitol.

Supercritical fluid extraction in isolation of cyclitols and sugars from chamomile flowers

“…After the GC-MS analysis of chamomile extracts five sugars: D-(+)-xylose, D-mannose, D-(+)-galactose, D-glucose, D-fructose, two sugar alcohols: D-mannitol and D-sorbitol) and seven cyclitols: D-pinitol, chiro-inositol, neo-inositol, D-(−)-bornesitol, ononitol, scyllo-inositol, and myo-inositol were identified in each extract. Some of the identified sugars like: xylose, fructose and glucose appeared in their isomeric forms with two or three peaks, as other studies confirmed before [27][28][29][30]. Table 4 presents the quantities of each sugar and cyclitol obtained with different extraction parameters.…”

Correlation Study of Honey Regarding their Physicochemical Properties and Sugars and Cyclitols Content

“…In terms of the sample preparation for the detection of cyclitols and sugars, honey samples can be easily handled, since just a dilution in water is required. Conversely, for the analysis of cyclitols and sugars from plant material, an elaborated procedure is required in terms of both, the solvent used and the extraction techniques [15][16][17][18][19][20].…”

Simultaneous Determination of Cyclitols and Sugars Following a Comprehensive Investigation of 40 Plants

“…Currently, several analytical methods are available for quantification of sugars and cyclitols (Al-Suod et al 2017, 2018a, Al-Suod et al 2019. High-performance liquid chromatography (HPLC) with different detectors was used for the analysis of this type of compounds (Filip et al 2016;Márquez-Sillero et al 2013;Shanmugavelan et al 2013).…”