Pressurized hot water extraction, structural properties, biological effects, and in vitro microbial fermentation characteristics of sweet tea polysaccharide

Lei, Jing; Li, Wei; Fu, Meng-Xi; Wang, Anqi; Wu, Ding‐Tao; Guo, Hongliang; Hu, Yichen; Gan, Ren‐You; Zou, Liang; Liu, Ying

doi:10.1016/j.ijbiomac.2022.10.094

Cited by 17 publications

(5 citation statements)

References 57 publications

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“…Figure 3 displays that all samples exhibited similar IR spectra, indicating they possessed similar preliminary chemical structure characteristics. Precisely, the absorption peak at around 3435 and 2947 cm −1 were the two typical characteristic peaks of polysaccharides, which were triggered by the stretching vibrations of O-H and C-H groups, respectively [43]. The absorption peaks near 1650 and 1448 cm −1 were related to the stretching vibrations of asymmetric and symmetric C=O groups, respectively [44], further confirming that TPs was an acidic polysaccharide.…”

Section: Ft-ir Spectramentioning

confidence: 70%

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

Xia

Liu

Sun

et al. 2023

Foods

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Deep eutectic solvent as a new green and safe solvent system has attracted more and more attention in recent years. In this study, three deep eutectic solvents (DES) were combined with ultrasound irradiation to extract tea polysaccharides (TPs) from Anji white tea, which was compared with conventional hot water extraction (HW). The physicochemical, structural, and biological properties of TPs extracted by ultrasound-assisted DES and hot water (HWP) were further investigated. Results showed that the DES system composed of choline chloride and 1,6-hexanediol (CH) with the molar ratio of 1:2 exhibited the optimal extraction yield (19.18%) and in vitro antioxidant activities for TPs (CHP). Furthermore, compared to the HWP, the CHP had a higher extraction yield and total carbohydrate content and a lower molecular weight. Monosaccharide composition analysis displayed that the molecular structure of CHP exhibited more arabinose but less glucose, mannose, galacturonic acid, and glucuronic acid than HWP. Little difference was observed in the preliminary structural characteristics between HWP and CHP from Fourier transform infrared analysis. Besides, CHP possessed better α-glucosidase inhibitory and hypoglycemic activity in L6 cells than HWP. Therefore, the ultrasound-assisted DES extraction method can be a promising strategy for extracting TPs with excellent bioactivities for future applications in functional foods.

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Section: Ft-ir Spectramentioning

confidence: 70%

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

Xia

Liu

Sun

et al. 2023

Foods

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“…As listed in Table 3 , the contents of sugar and uronic acids were 72.58% and 18.98%, respectively, which indicated that the dominant polysaccharides in NRF were acidic polysaccharides [ 12 ]. The protein content was found at a content of 8.69%.…”

Section: Resultsmentioning

confidence: 99%

“…The protein content was found at a content of 8.69%. These results indicated the existence of a polysaccharide–protein complex, which showed favorable biological activities [ 12 ]. In addition, the conjugated polyphenolics, which were demonstrated to be effective antioxidants and antidiabetics, were found at a level of 6.32 mg GAE/g [ 13 , 14 ].…”

Section: Resultsmentioning

confidence: 99%

Polysaccharides from Nitraria retusa Fruit: Extraction, Purification, Structural Characterization, and Antioxidant Activities

et al. 2023

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Polysaccharides are important bioactive components of Nitraria retusa fruit (NRF). In this study, the ultrasonic-assisted extraction (UAE) conditions of polysaccharides from Nitraria retusa fruit (NRFPs) were optimized by response surface methodology (RSM). The structural characteristics and antioxidant activity were investigated. The maximum NRFPs yield of 3.35% was obtained under the following optimal conditions: temperature of 59.5 °C, time of 30.5 min, liquid-to-solid ratio of 19.5 mL/g. Three polysaccharide fractions, NRFP-1 (20.01 kDa), NRFP-2 (28.96 kDa), and NRFP-3 (67.45 kDa), were isolated. Glucose, galactose, and arabinose in different percentages were identified as the primary monosaccharide units. The Fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance (NMR) analysis indicated the presence of α- and β-glycosidic bonds in NRFPs. The NRFP-3 exhibited the highest scavenging activities against DPPH, ABTS, -OH free radicals, and Fe+3-reducing activity.

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“…The yields of TBP-PD enhanced when the pressure increased from 50 MPa to 110 MPa, while obviously declining with a further increase in pressure. This result could be due to the physical degradation of pectic polysaccharides under excessively high pressure [27]. Finally, the optimal time, water content in the DES, pressure, and solid/liquid ratio were determined to be 10 min, 55%, 110 MPa, and 40 mL/g, respectively.…”

Section: Extraction Optimization Of Deep-eutectic-solvent-assisted Ex...mentioning

confidence: 99%

Efficient and Selective Extraction of Rhamnogalacturonan-I-Enriched Pectic Polysaccharides from Tartary Buckwheat Leaves Using Deep-Eutectic-Solvent-Based Techniques

Wu,

Lei,

et al. 2024

Foods

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Tartary buckwheat green leaves are considered to be among the most important by-products in the buckwheat industry. Although Tartary buckwheat green leaves are abundant in pectic polysaccharides, their potential applications in the food industry are quite scarce. Therefore, to promote their potential applications as functional or fortified food ingredients, both deep-eutectic-solvent-assisted extraction (DESE) and high-pressure-assisted deep eutectic solvent extraction (HPDEE) were used to efficiently and selectively extract pectic polysaccharides from Tartary buckwheat green leaves (TBP). The results revealed that both the DESE and HPDEE techniques not only improved the extraction efficiency of TBP but also regulated its structural properties and beneficial effects. The primary chemical structures of TBP extracted using different methods were stable overall, mainly consisting of homogalacturonan and rhamnogalacturonan-I (RG-I) pectic regions. However, both the DESE and HPDEE methods could selectively extract RG-I-enriched TBP, and the proportion of the RG-I pectic region in TBP obviously improved. Additionally, both the DESE and HPDEE methods could improve the antioxidant and anti-glycosylation effects of TBP by increasing its proportion of free uronic acids and content of bound polyphenolics and reducing its molecular weight. Moreover, both the DESE and HPDEE methods could partially intensify the immunostimulatory effect of TBP by increasing its proportion of the RG-I pectic region. These findings suggest that DES-based extraction techniques, especially the HPDEE method, can be promising techniques for the efficient and selective extraction of RG-I-enriched TBP.

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Pressurized hot water extraction, structural properties, biological effects, and in vitro microbial fermentation characteristics of sweet tea polysaccharide

Cited by 17 publications

References 57 publications

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method

Polysaccharides from Nitraria retusa Fruit: Extraction, Purification, Structural Characterization, and Antioxidant Activities

Efficient and Selective Extraction of Rhamnogalacturonan-I-Enriched Pectic Polysaccharides from Tartary Buckwheat Leaves Using Deep-Eutectic-Solvent-Based Techniques

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