Pressure–volume relationship in isolated working heart with crystalloid perfusate in swine and imaging the valve motion

Abstract

The systolic and diastolic functions of an isolated heart were preserved at an acceptable level for 180 min. The practical reliability of the swine working heart model was demonstrated. This model will be used reliably for the investigation of the interaction of valve and cardiac functions.

Mitral valve motion assessed by high-speed video camera in isolated swine heart

“…We have recently reported our isolated and working swine heart model that examines the valve motion precisely by a high-speed digital video camera system recording the images at 250 frames per second (fps) [25]. By use of this modality, the present study aimed (1) to delineate the motion of the mitral leaflets, chords and annulus throughout the cardiac cycle, and (2) to elucidate the influence of alterations in loading conditions on leaflet excursion.…”

Padronização de modelo de coração isolado "working heart" com circulação parabiótica

“…Araki et al [18] published standardized model in isolated swine heart "working heart" perfused with crystalloid solution, and they measured the maximum elastance and strength of the ventricle. In this study, the results of the contractility parameters obtained "in situ" were consistent with the results obtained in the stabilized isolated heart, before treatments, emphasize the applicability of this model.…”

“…The piezoelectric crystals of the left ventricle were connected to a synchronizer (TRX6, Sonometrics, London, Ontario, Canada) to calculate the left ventricle volume and to obtain the volume-pressure curves and their respective contractility indexes. [14][15][16][17][18][19][20][21][22] After 20 minutes of passive reperfusion, the perfusion was converted to "working state." The flow from the support animal to the isolated heart aorta was interrupted, and the blood was drained and forwarded to the cardiotomy reservoir and, from this (acting as a preload reservoir), it was forwarded to the cannulated left atrium of the isolated heart.…”

Extracorporeal perfusion of isolated organs of large animals – Bridging the gap between in vitro and in vivo studies

“…The isolated heart can also be perfused in the working heart mode, i.e., the perfusate enters the left atrium via the pulmonary vein, flows into the left ventricle and onwards into the aorta (Chinchoy et al, 2000;Araki et al, 2005). The right side of the heart is not included in the latter system as for this a biventricular working heart set-up would be necessary, in which the right heart performs a physiological low pressure ejection (Demmy et al, 1992;Chinchoy et al, 2000) Uterus Another application of isolated organ perfusion is represented by the use of the bovine uterus for the study of mucosal inflammation (Tab.…”