Pressure-programming of a binary, on-line mixed mobile phase for capillary column SFC using a packed column SFC pumping system

Anton, K.; Periclès, N.; Fields, Steven M.; Widmer, Hans

doi:10.1007/bf02268156

Cited by 18 publications

(2 citation statements)

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“…Albeit numerous system improvements have been described by Widmer [261][262][263][264], they were not able to overcome a serious lack of robustness and reproducibility. This has somewhat limited its use for analytical purpose as well as in the pharmaceutical industry where it has remained in the shadow of LC considered today as a reference technique.…”

Section: Advantages and Limitations Of Sfcmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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Section: Advantages and Limitations Of Sfcmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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“…in Basel published instrument details in 1980. [36][37][38][39] Modern packed column instruments use multiple high pressure reciprocating pumps operated as flow sources. The system pressure is independently controlled through the use of electronic back pressure regulators.…”

Section: Mixermentioning

confidence: 99%

Analytical SFC for Impurities

2014

Supercritical Fluid Chromatography

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Capillary column SFC instrumentation based on piston pumps with a programmable back‐pressure regulator for on‐line mixing of mobile phases

Anton¹,

Periclès²,

Widmer³

1989

J. High Resol. Chromatogr.

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An alternative phase deliverysystem based on piston pumps and a back-pressure regulator has been developed for capillary column SFC.Thechromatographyis not affected bythefast piston pump refill. A homogeneous on-line mixing of binary phases with simultaneous pressure programming is easily accessible without any additional computing. Acceptable reproducibilitiesMethod development requires the capability of fast change in modifier concentration or exchange of mobile phase components. Therefore, an on-line phase mixing is preferred rather than an off-line procedure.In principle, pressure (density), temperature, and mobile phase composition gradients can influence the separanon process in CSFC cases, the usefulness of composltlon gradents in CSFC has not yet been shown However, results in packed column SFC show (< 3.5% RSD for external and < 2.0% RSD for internal standard methods) were found with mixtures of P-propanol/CO* as mobile phases using UV detection and split ratios of 1 :GO and 1 :120.Variation and control ofthesplit areeasilydone bysimple flow rate volumetric changes.

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Pressure-programming of a binary, on-line mixed mobile phase for capillary column SFC using a packed column SFC pumping system

Cited by 18 publications

References 5 publications

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Analytical SFC for Impurities

Capillary column SFC instrumentation based on piston pumps with a programmable back‐pressure regulator for on‐line mixing of mobile phases

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