Pressure drop in CIM disk monolithic columns

Polymethacrylate monoliths, specifically poly(glycidyl methacrylate-co-ethylene dimethacrylate) or poly(GMA-co-EDMA) monoliths, are a new generation of chromatographic supports and are significantly different from conventional particlebased adsorbents, membranes, and other monolithic supports for biomolecule purification. Similar to other monoliths, polymethacrylate monoliths possess large pores which allow convective flow of mobile phase and result in high flow rates at reduced pressure drop, unlike particulate supports. The simplicity of the adsorbent synthesis, pH resistance, and the ease and flexibility of tailoring their pore size to that of the target biomolecule are the key properties which differentiate polymethacrylate monoliths from other monoliths. Polymethacrylate monoliths are endowed with reactive epoxy groups for easy functionalization (with anion-exchange, hydrophobic, and affinity ligands) and high ligand retention. In this review, the structure and performance of polymethacrylate monoliths for chromatographic purification of biomolecules are evaluated and compared to those of other supports. The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through-put biomolecule purification on semi-preparative and preparative scales.

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“…Combining equations (11), (12), (13), and (14), and differentiating DP 0 DP with respect to dn gives:…”

Section: Scenariomentioning

confidence: 99%

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Roberts

Ongkudon

Forde

et al. 2009

J of Separation Science

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“…The use of ultra-short monolithic columns or , discs' (from 4 to 50 mm in length) attracts attention due to their potential for rapid separations of relatively simple samples, with applications in several areas, including biochemistry, pharmaceutical analysis and affinity chromatography [16][17][18]. At the same time, low column backpressures with such ultra-short columns allow the application of high flow rates (up to 5 mL/min) in order to further reduce retention times.…”

Section: Separation Of Nucleotides On Ultra-short Columnsmentioning

confidence: 99%

Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient

Nesterenko

Guivarc'h

Duffy

et al. 2007

J of Separation Science

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An RP monolithic column coated with an amphoteric carboxybetaine type surfactant has been used with a combined triple eluent concentration, pH and flow gradient ion chromatography technique for the simultaneous separation of up to 18 nucleotides, nucleosides and nucleobases. The separation of up to eight precursors on a 1 cm long monolithic microcolumn using the combined gradient approach is also shown. The method was applied to the separation of the above nucleic acid precursors in perchloric acid extracts of yeastolates samples.

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“…Such distribution provides enough surface to obtain high dynamic binding capacity as well as further reduces the pressure drop. Recently, it was demonstrated that the structure in fact exhibits a certain level of self-similarity (between 1.03 and 2.75) that results in two-fold lower pressure in comparison to an equivalent particle bed [62].…”

Section: Structure Optimizationmentioning

confidence: 99%

Convective Interaction Media short monolithic columns: Enabling chromatographic supports for the separation and purification of large biomolecules

Barut¹,

Podgornik

Brne

et al. 2005

J of Separation Science

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New therapeutics that are being developed rely more and more on large and complex biomacromolecules like proteins, DNA, and viral particles. Manufacturing processes are being redesigned and optimized both upstream and downstream to cope with the ever-increasing demand for the above target molecules. In downstream processing, LC still represents the most powerful technique for achieving high yield and high purities of these molecules. In most cases, however, the separation technology relies on conventional particle-based technology, which has been optimized for the purification of smaller molecules. New technologies are, therefore, needed in order to push the downstream processing ahead and into the direction that will provide robust, productive, and easy to implement methods for the production of novel therapeutics. New technologies include the renaissance of membranes, various improvements of existing technologies, but also the introduction of a novel concept--the continuous bed or monolithic stationary phases. Among different introduced products, Convective Interaction Media short monolithic columns (SMC) that are based on methacrylate monoliths exhibit some interesting features that make them attractive for these tasks. SMC can be initially used for fast method development on the laboratory scale and subsequently efficiently transferred to preparative and even more importantly to industrial scale. A brief historical overview of methacrylate monoliths is presented, followed by a short presentation of theoretical considerations that had led to the development of SMC. The design of these columns, as well as their scale-up to large units, together with the methods for transferring gradient separations from one scale to another are addressed. Noninvasive methods that have been developed for the physical characterization of various batches of SMC, which fulfill the regulatory requirements for cGMP production, are discussed. The applications of SMC for the separation and purification of large biomolecules, which demonstrate the full potential of this novel technology for an efficient downstream processing of biomolecules, are also presented.

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Pressure drop in CIM disk monolithic columns

Cited by 58 publications

References 13 publications

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules

Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient

Convective Interaction Media short monolithic columns: Enabling chromatographic supports for the separation and purification of large biomolecules

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