Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system

Furse, Samuel; Brooks, Nicholas J.; Woscholski, Rüdiger; Gaffney, Piers R. J.; Templer, Richard H.

doi:10.1016/j.cdc.2016.08.001

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…DOPC and DOPE are representatives of two bulk lipids found across practically all eukaryotes [43][44][45][46], with PE also being common in prokaryotes [37,47]. The phase behavior of DOPE and DOPC has been investigated in considerable detail [39,40,42,48,49], making deviations from the typical behavior of these systems generally straightforward to identify [29,38,50,51]. Therefore, they are useful for characterizing the behavior of lipids in response to minor additives [29,36,38].…”

Section: Resultsmentioning

confidence: 99%

“…The phase behavior of DOPE and DOPC has been investigated in considerable detail [39,40,42,48,49], making deviations from the typical behavior of these systems generally straightforward to identify [29,38,50,51]. Therefore, they are useful for characterizing the behavior of lipids in response to minor additives [29,36,38]. Doping with 1%, 5% and 10% of synthetically pure samples of compounds 1-5 was done to characterize modulations in the lipid-lipid interactions in the DOPC and DOPE assays.…”

Section: Resultsmentioning

confidence: 99%

“…It has also been documented that transferring oleic acid to the cell membrane using protein fatty-acid complexes, liprotides, cause cancer cell death through disruptive effects on the membranes [34,35]. These phenomena, along with recent evidence indicating that even small changes to the composition of lipid systems may have a pronounced effect on their phase behavior [29,[36][37][38], prompted us to investigate malonganone J, agelasine D, and agelasine analogs using hydrated dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylethanolamine (DOPE) model lipid systems doped with these compounds (1)(2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 97%

“…It is tempting to speculate that interference with these proteins is central to their mode of action (MOA). While MOA evidence exists for individual cases [26,28,29], a comprehensive picture has yet to emerge. Here, we investigated an alternative or complementary MOA for agelasine and malonganenone compounds: the perturbation of the lipid membrane.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

et al. 2020

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Marine sponges and soft corals have yielded novel compounds with antineoplastic and antimicrobial activities. Their mechanisms of action are poorly understood, and in most cases, little relevant experimental evidence is available on this topic. In the present study, we investigated whether agelasine D (compound 1) and three agelasine analogs (compound 2–4) as well as malonganenone J (compound 5), affect the physical properties of a simple lipid model system, consisting of dioleoylphospahtidylcholine and dioleoylphosphatidylethanolamine. The data indicated that all the tested compounds increased stored curvature elastic stress, and therefore, tend to deform the bilayer which occurs without a reduction in the packing stress of the hexagonal phase. Furthermore, lower concentrations (1%) appear to have a more pronounced effect than higher ones (5–10%). For compounds 4 and 5, this effect is also reflected in phospholipid headgroup mobility assessed using 31P chemical shift anisotropy (CSA) values of the lamellar phases. Among the compounds tested, compound 4 stands out with respect to its effects on the membrane model systems, which matches its efficacy against a broad spectrum of pathogens. Future work that aims to increase the pharmacological usefulness of these compounds could benefit from taking into account the compound effects on the fluid lamellar phase at low concentrations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

et al. 2020

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“…PG is relatively unusual amongst lipids in that it is both invariably type 0 and anionic. Other major anionic lipids, PA [27][28][29], PI [140,141], PI-4-P [142,143] and CL [104,144], can behave as type II lipids, and with the arguable exception of PI [77], typically do so under physiological conditions. Thus, the presence of PG confers a negative charge on a membrane without increasing stored curvature elastic stress.…”

Section: Discussionmentioning

confidence: 99%

Is phosphatidylglycerol essential for terrestrial life?

Furse

2016

J Chem Biol

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Lipids are of increasing importance in understanding biological systems. Lipids carrying an anionic charge are noted in particular for their electrostatic interactions with both proteins and divalent cations. However, the biological, analytical, chemical and biophysical data of such species are rarely considered together, limiting our ability to assess the true role of such lipids in vivo. In this review, evidence from a range of studies about the lipid phosphatidylglycerol is considered. This evidence supports the conclusions that this lipid is ubiquitous in living systems and generally of low abundance but probably fundamental for terrestrial life. Possible reasons for this are discussed and further questions posed.

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Do lipids shape the eukaryotic cell cycle?

Furse

Shearman

2018

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Successful passage through the cell cycle presents a number of structural challenges to the cell. Inceptive studies carried out in the last five years have produced clear evidence of modulations in the lipid profile (sometimes referred to as the lipidome) of eukaryotes as a function of the cell cycle. This mounting body of evidence indicates that lipids play key roles in the structural transformations seen across the cycle. The accumulation of this evidence coincides with a revolution in our understanding of how lipid composition regulates a plethora of biological processes ranging from protein activity through to cellular signalling and membrane compartmentalisation. In this review, we discuss evidence from biological, chemical and physical studies of the lipid fraction across the cell cycle that demonstrate that lipids are well-developed cellular components at the heart of the biological machinery responsible for managing progress through the cell cycle. Furthermore, we discuss the mechanisms by which this careful control is exercised.

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Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system

Cited by 7 publications

References 29 publications

Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

Is phosphatidylglycerol essential for terrestrial life?

Do lipids shape the eukaryotic cell cycle?

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