Pressure-assisted electrokinetic injection for the stacking of biogenic amines gives enhancement factor up to 1000 in CE with UV detection

Yang, Xue; He, Lili; Xu, Zhongqi

doi:10.1039/d2ay00430e

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…Hybrid modes (such as pressure-assisted EK injection, PAEKI) have been described and used to improve the sensitivity of the analysis of proteins [387], antibiotics [388,389], biogenic amines [390], or inorganic contaminants [391]. Although the approach was applied to a nanofluidic device, electro-preconcentration diagrams were used to optimize molecular enrichment with low counter pressure using albumin as a model analyte, reaching preconcentration factors of 70 after only 2 min [387].…”

Section: Injection Modes To Improve the Sensitivitymentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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Section: Injection Modes To Improve the Sensitivitymentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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“…On the other hand, Yang et al. developed a method to enhance the detection sensitivity of BAs from chicken by CE–UV applying pressure‐assisted electrokinetic injection (PAEKI) [33]. CE coupled to PAEKI produced a preconcentration of 13 BAs from chicken causing a counterbalanced electroosmotic flow (EOF) under positive injection voltage (7.8 kV) and employing an external pressure in the opposite direction (−0.1 psi) with an injection time of 0.70 min.…”

Section: Amino Acids Biogenic Amines Heterocyclic Amines and Other Ha...mentioning

confidence: 99%

“…CE coupled to PAEKI produced a preconcentration of 13 BAs from chicken causing a counterbalanced electroosmotic flow (EOF) under positive injection voltage (7.8 kV) and employing an external pressure in the opposite direction (−0.1 psi) with an injection time of 0.70 min. In this manner, the LODs of BAs employing CE–PAEKI enhanced from 8.7–24.3 nmol/L using indirect UV detection to 0.4–4.5 nmol/L with direct UV detection compared with hydrodynamic injection, providing an enrichment factor up to 1000 [33].…”

Section: Amino Acids Biogenic Amines Heterocyclic Amines and Other Ha...mentioning

confidence: 99%

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

Domínguez‐Rodríguez,

Montero,

Herrero

et al. 2023

Electrophoresis

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This work presents a revision of the main applications of capillary electromigration (CE) methods in food analysis and Foodomics. Papers that were published during the period March 2021 to March 2023 are included. The work shows the multiple CE methods that have been developed and applied to analyze different types of molecules in foods and beverages. Namely, CE methods have been applied to analyze amino acids, biogenic amines, heterocyclic amines, peptides, proteins, phenols, polyphenols, pigments, lipids, carbohydrates, vitamins, DNAs, contaminants, toxins, pesticides, additives, residues, small organic and inorganic compounds, and other minor compounds. In addition, new CE procedures to perform chiral separation and for evaluating the effects of food processing as well as the last developments of microchip CE and new applications in Foodomics will be also discussed. The new procedures of CE to investigate food quality and safety, nutritional value, storage, and bioactivity are also included in the present review work.

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A Review on the Determination of Biogenic Amines in Fresh and Processed Fish Products using HPLC, LC-MS/MS and Other Chromatographic Methods

Ahangari

Tavassoli

Khezerlou

et al. 2023

CCHTS

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Biogenic amines (BAs) are compounds deemed to be foodstuff contaminants and are the cause of poisoning or allergy. The main BAs found in foods include histamine, tyramine, putrescine, cadaverine, spermine and spermidine. The number of poisoning cases related to BAs in food has increased, which is reinforcing the need for BAs detection to ensure food safety. BAs are found in varying quantities in different foods such as fish, fruits, meat, cheese, vegetables, beer, and wine. Currently, different analytical techniques are used for BAs detection, as well as sample treatment methods that allow greater sensitivity, higher analyzing speed and lower detection limits. Moreover, BAs can be precursors of nitrosamines, which have been associated with mutagenic and carcinogenic activity. This review aims to provide a general approach to the different detection techniques of the BAs in foods, their concentrations and treatment methods.

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Pressure-assisted electrokinetic injection for the stacking of biogenic amines gives enhancement factor up to 1000 in CE with UV detection

Abstract: Pressure-assisted electrokinetic injection (PAEKI) was applied for stacking of positively charged biogenic amines (BAs) to improve the sensitivity of capillary electrophoresis (CE).

Cited by 3 publications

References 32 publications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

A Review on the Determination of Biogenic Amines in Fresh and Processed Fish Products using HPLC, LC-MS/MS and Other Chromatographic Methods

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