Pressure and Fluorescence Dual Signal Readout CuO-NiO/C Heterojunction Nanofibers-Based Nanoplatform for Imaging and Detection of Target Cancer Cells in Blood

Abstract

The development of multifunctional nanoplatform, which integrates biosensing and imaging diagnosis, for accurate identification of cancer cells in blood or other bodily fluids is critical for early cancer diagnosis and treatment. Herein, by using a facile single spinneret electrospinning technique followed by thermal treatment, CuO-NiO/C heterojunction nanofiber was synthesized. The resultant CuO-NiO/C nanofibers exhibited excellent catalytic activity toward ammonia borane (AB) hydrolysis, producing a marked pressure increase in a closed reaction vessel. Moreover, the resultant nanofibers showed highly efficient catalytic activity for reduction of rhodamine 6G (Rh6G, fluorescent molecule), accompanied by the fluorescence quenching. On the basis of these findings, a novel sensing platform utilizing folic-acid (FA) conjugated CuO-NiO/C nanofibers as an artificial enzyme and recognition element for fluorescence imaging and pressurebased (pressure meter) detection of folate receptor (FR)-positive cancer cells was developed. The detection limit is as low as 50 cells/mL. What's more, the clinical applicability of the sensor was also proven to be suitable for the sensing cancer cells in whole blood. The most important advantage of the synthesized nanofibers based-probe is the combination of pressure meter and fluorescence imaging to specifically see and sensitively count the cancer cells, paving a new way in early liver cancer diagnosis.

Most recent developments in electrospun magnetic nanofibers: A review

“…94 Similarly, Chang et al 95 found Fe 2 O 3 /BiOI electrospun hollow nanofibers to show high catalytic performance even under visible light for rhodamine B decomposition. Good catalytic properties and cycle stability was also recognized for PVA/ poly(acrylic acid)/Fe 3 O 4 /MXene@Ag nanoparticle composites, produced by electrospinning, 96 as well as CuO-NiO/carbon nanofibers which were carbonized after electrospinning 97 and Bi 2 MoO 6 /ZnFe 2 O 4 heterojunction nanofibers which were tested by rhodamine B degradation. 98 Electrospun magnetic nanofibers from a Fe 3 O 4 /PVA core and a poly(3-hydroxybutyrate) and PCL shell were decorated with TiO 2 nanoparticles by electrospraying, resulting in good photocatalytic activity under UV light.…”

Platinum Nanozyme-Triggered Pressure-Based Immunoassay Using a Three-Dimensional Polypyrrole Foam-Based Flexible Pressure Sensor

“…Benefiting from emerging nanomaterials, alternative approaches based on diverse conversion principles have been proposed, such as temperature, , distance, and color. , Among these parameters, air pressure as a classical physical element widely exists in bio/chemical reactions. , Yang’s group inventively exploited novel pressure-based bioassays using a handheld pressure meter. In these studies, a typical gas-generating reaction, that is, the decomposition of H 2 O 2 to O 2 , was chosen as the link between biomolecule recognition and signal readout. − Compared with other gas-generating reactions (e.g., hydrogen and carbon dioxide), this reaction is environment-friendly and has highly efficient liquid–gas phase conversion potency. Typically, platinum nanoparticles (PtNPs) have excellent peroxidase-like activity and can be utilized as the peroxidase mimics (nanozymes) to catalyze the reduction of H 2 O 2 .…”

“…In these studies, a typical gas-generating reaction, that is, the decomposition of H 2 O 2 to O 2 , was chosen as the link between biomolecule recognition and signal readout. 17−20 Compared with other gas-generating reactions (e.g., hydrogen 21 and carbon dioxide 22 ), this reaction is environment-friendly and has highly efficient liquid−gas phase conversion potency. Typically, platinum nanoparticles (PtNPs) have excellent peroxidase-like activity and can be utilized as the peroxidase mimics (nanozymes) to catalyze the reduction of H 2 O 2 .…”

Self‐assembled magnetic nanomaterials: Versatile theranostics nanoplatforms for cancer

“…[8][9][10] Nanomaterials with pharmaceutical properties could be prepared by using many kinds of materials, including liposomes, polymers, biomacromolecules, and many other inorganic nanomaterials. [2,5,[11][12][13][14][15][16][17][18][19][20] In addition, the activity of these nanomaterials can be adjusted and improved by surface modification, [2,10,21] which will promote their in-depth study in biomedicine. The development of many advanced nanosystems in biomedicine have been inspired by these interesting properties.…”