Prespore cell inducing factor, ψ factor, controls both prestalk and prespore gene expression in <i>Dictyostelium</i> development

Yamada, Yoko; Minamisawa, Hiroshi; Fukuzawa, Masashi; Kawata, Takefumi; Oohata, Akiko A.

doi:10.1111/j.1440-169x.2010.01177.x

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…Furthermore, the identification of the traA227P→L missense mutation within PA14 highlights the importance of this domain for function (Figure 4). We also note that Dictyostelium discoideum , a eukaryotic soil slime mold that exhibits similar multicellular behaviors as M. xanthus [41], produces two secreted signals, called DicA1 (PsiF) and PsiA, whose proteins contain PA14 domains followed by cysteine-rich repeats (Pfam00526) of various lengths that show some resemblance to TIGR04201 [21], [27], [42], [43]. Thus, M. xanthus and other microbes, including eukaryotes, appear to utilize PA14 encoding proteins as extracellular signaling and recognition molecules to mediate social interactions.…”

Section: Discussionmentioning

confidence: 90%

Cell Contact–Dependent Outer Membrane Exchange in Myxobacteria: Genetic Determinants and Mechanism

et al. 2012

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Biofilms are dense microbial communities. Although widely distributed and medically important, how biofilm cells interact with one another is poorly understood. Recently, we described a novel process whereby myxobacterial biofilm cells exchange their outer membrane (OM) lipoproteins. For the first time we report here the identification of two host proteins, TraAB, required for transfer. These proteins are predicted to localize in the cell envelope; and TraA encodes a distant PA14 lectin-like domain, a cysteine-rich tandem repeat region, and a putative C-terminal protein sorting tag named MYXO-CTERM, while TraB encodes an OmpA-like domain. Importantly, TraAB are required in donors and recipients, suggesting bidirectional transfer. By use of a lipophilic fluorescent dye, we also discovered that OM lipids are exchanged. Similar to lipoproteins, dye transfer requires TraAB function, gliding motility and a structured biofilm. Importantly, OM exchange was found to regulate swarming and development behaviors, suggesting a new role in cell–cell communication. A working model proposes TraA is a cell surface receptor that mediates cell–cell adhesion for OM fusion, in which lipoproteins/lipids are transferred by lateral diffusion. We further hypothesize that cell contact–dependent exchange helps myxobacteria to coordinate their social behaviors.

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Section: Discussionmentioning

confidence: 90%

Cell Contact–Dependent Outer Membrane Exchange in Myxobacteria: Genetic Determinants and Mechanism

et al. 2012

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“…PsiI is a PA14 domain-containing protein similar to prespore inducing factor PsiA [117]. During normal development, the psiA gene is highly expressed in scattered cells at the mound stage and in prespore cells at the onset of culmination, and regulates both prespore and prestalk/stalk cell differentiation [118]. Last but not least, we have identified four transmembrane proteins, one metabolic protein, two LIM-type zinc finger-containing proteins (DDB0233465 and DDB0238324) and 15 unique proteins with unknown functions that have not been previously annotated or studied (Table S6).…”

Section: Resultsmentioning

confidence: 99%

Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins in Dictyostelium Discoideum

González-Velasco

Rivas

Lacal

2019

Cells

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Cyclic AMP acts as a secondary messenger involving different cellular functions in eukaryotes. Here, proteomic and transcriptomic profiling has been combined to identify novel early developmentally regulated proteins in eukaryote cells. These proteomic and transcriptomic experiments were performed in Dictyostelium discoideum given the unique advantages that this organism offers as a eukaryotic model for cell motility and as a nonmammalian model of human disease. By comparing whole-cell proteome analysis of developed (cAMP-pulsed) wild-type AX2 cells and an independent transcriptomic analysis of developed wild-type AX4 cells, our results show that up to 70% of the identified proteins overlap in the two independent studies. Among them, we have found 26 proteins previously related to cAMP signaling and identified 110 novel proteins involved in calcium signaling, adhesion, actin cytoskeleton, the ubiquitin-proteasome pathway, metabolism, and proteins that previously lacked any annotation. Our study validates previous findings, mostly for the canonical cAMP-pathway, and also generates further insight into the complexity of the transcriptomic changes during early development. This article also compares proteomic data between parental and cells lacking glkA, a GSK-3 kinase implicated in substrate adhesion and chemotaxis in Dictyostelium. This analysis reveals a set of proteins that show differences in expression in the two strains as well as overlapping protein level changes independent of GlkA.

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“…Oohata has described low-density monolayer conditions that cause most cells to differentiate into prespore cells (Oohata, 1995; Oohata et al, 1997). A prespore inducing factor has been isolated from the media of cells developed under these conditions (Yamada et al, 2010). It is possible that PIF is this factor.…”

Section: Discussionmentioning

confidence: 99%

AmpA protein functions by different mechanisms to influence early cell type specification and to modulate cell adhesion and actin polymerization in Dictyostelium discoideum

2013

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The Dictyostelium discoideum ampA gene encodes a multifunctional regulator protein that modulates cell–cell and cell–substrate adhesions and actin polymerization during growth and is necessary for correct cell type specification and patterning during development. Insertional inactivation of the ampA gene results in defects that define two distinct roles for the ampA gene during development. AmpA is necessary in a non-cell autonomous manner to prevent premature expression of a prespore gene marker. It is also necessary in a cell autonomous manner for the anterior like cells, which express the ampA gene, to migrate to the upper cup during culmination. It is also necessary to prevent excessive cell–cell agglutination when cells are developed in a submerged suspension culture. Here, we demonstrate that a supernatant source of AmpA protein, added extracellularly, can prevent the premature mis-expression of the prespore marker. Synthetic oligopeptides are used to identify the domain of the AmpA protein that is important for preventing cells from mis-expressing the prespore gene. We further demonstrate that a factor capable of inducing additional cells to express the prespore gene marker accumulates extracellularly in the absence of AmpA protein. While the secreted AmpA acts extracellularly to suppress prespore gene expression, the effects of AmpA on cell agglutination and on actin polymerization in growing cells are not due to an extracellular role of secreted AmpA protein. Rather, these effects appear to reflect a distinct cell autonomous role of the ampA gene. Finally, we show that secretion of AmpA protein is brought about by elevating the levels of expression of ampA so that the protein accumulates to an excessive level.

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Prespore cell inducing factor, ψ factor, controls both prestalk and prespore gene expression in Dictyostelium development

Cited by 4 publications

References 36 publications

Cell Contact–Dependent Outer Membrane Exchange in Myxobacteria: Genetic Determinants and Mechanism

Cell Contact–Dependent Outer Membrane Exchange in Myxobacteria: Genetic Determinants and Mechanism

Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins in Dictyostelium Discoideum

AmpA protein functions by different mechanisms to influence early cell type specification and to modulate cell adhesion and actin polymerization in Dictyostelium discoideum

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