Preserving life of moss Ptychomitrium under very high pressure

Nishihira, N.; Shindo, A.; Saigusa, Masahiro; Ono, F.; Matsushima, Yasushi; Mori, Yojiro; Takarabe, Kenichi; Saini, N. L.; Yamashita, Masamichi

doi:10.1016/j.jpcs.2010.03.018

Cited by 13 publications

(18 citation statements)

References 6 publications

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“…The temperature in the incubator was set constant at 25 ı C with white light of the intensity 2,500 Lx (Nishihira et al 2010). …”

Section: Results For Ptychomitriummentioning

confidence: 99%

“…Nobody would have believed that small creatures can withstand against such a very high pressure of several GPa order for such a long time of more than a few hours until a several experiments were conducted concerning the search for life under very high hydrostatic pressure (Ono et al 2008;Horikawa et al 2009;Nishihira et al 2010). In our experiments all kinds of living samples, both animals and plants tested were proven to keep their lives after exposure to such a very high hydrostatic pressure of 7.5 GPa.…”

Section: Overviewmentioning

confidence: 88%

“…Among the candidates for these experiments, the samples that showed the strongest tolerance against the very high pressure were moss spores of Ptychomitrium and Venturiella. It was shown (Ono et al 2010a;Nishihira et al 2010) that the spores of these mosses can survive under very high pressure, as high as 7.5 GPa, for up to 144 h. Then, a big question arose that what mechanism made the living creatures so strong against very high pressure. To make a contribution to open up this question, we have made a closer investigation to the moss spores from a biophysical and biochemical point of view.…”

Section: Overviewmentioning

confidence: 99%

“…The seeded medium was kept in an incubator for 1-4 weeks or more under white light with an intensity of 2,500 Lx. These spores were investigated under an .6 (a) The Teflon capsule containing three spore placentas before exposure to high pressure, and (b) brought out from the pyrophillite cube after exposure to 7.5 GPa (Nishihira et al 2010;Ono et al 2010a) optical microscope. Figure 22.6a shows the teflon capsule containing three spore placentas before exposure to high pressure, and (b) shows after the pressure was released.…”

Section: Generation Of High Hydrostatic Pressure Up To Several Gpamentioning

confidence: 99%

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Moss Spores Can Tolerate Ultra-high Pressure

Ono

2015

Subcellular Biochemistry

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It was shown by the present authors' group that tardigrade can survive under very high hydrostatic pressure range of 7.5 GPa. In the case of small land plants, the tolerance to high pressure seems stronger than such a small animal, tardigrade. We have extended our experiments to two popular mosses, Ptychomitrium and Venturiella searching for lives under very high pressure range. Spore placentas of moss Ptychomitrium and Venturiella were sealed in a small Teflon capsule together with a liquid pressure medium. The capsule was put in the center of a pyrophillite cube, and the maximum pressure of 7.5 GPa was applied using a 250-t cubic anvil press. The pressure was kept constant at the maximum pressure for various duration of time between 6 and 144 h. After the pressure was released, the spores were seeded on agar medium, and incubated for one week and longer at 25 °C with white light of 2,000-2,500 Lx. It was proven that 80-90% of the spores of moss Ptychomitrium were alive and germinated after being exposed to the very high pressure of 7.5 GPa for up to 48 h. Furthermore, a relatively high germination rate of about 35% was retained even after exposure to 7.5 GPa for 144 h. It was also proven that 70-90% of the spores of moss Venturiella were alive and germinated after exposure to 7.5 GPa for up to 72 h. However, after exposed to 7.5 GPa for 144 h, only four individuals in a hundred were germinated. The pressure tolerances of the mosses Ptychomitrium and Venturiella were found to be much stronger than tardigrade. The experiments were extended to the ultra-high pressure range up to 20 GPa, and a few spores of Venturiella were germinated up to the length of 30 μm. The mechanisms for such strong tolerance of moss spores will be discussed on the bases of the quality of the applied hydrostatic pressure, degree of the physical deformation and extremophiles.

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“…The temperature in the incubator was set constant at 25 ı C with white light of the intensity 2,500 Lx (Nishihira et al 2010). …”

Section: Results For Ptychomitriummentioning

confidence: 99%

Section: Overviewmentioning

confidence: 88%

Section: Overviewmentioning

confidence: 99%

Section: Generation Of High Hydrostatic Pressure Up To Several Gpamentioning

confidence: 99%

See 2 more Smart Citations

Moss Spores Can Tolerate Ultra-high Pressure

Ono

2015

Subcellular Biochemistry

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“…. (Color online) Survival limit of exposed time at 7.5 GPa for white clover [10], Shepherd's purse [15], budding yeast [13], tardigrades [4], Artemia [6,7], Venturiella [9] and Ptychomitrium [8] investigated to date. …”

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confidence: 99%

Search for Lives under Very High Pressure and Extraordinary Strong Pressure-Tolerance of Animals and Plants

Ono

2017

The Review of High Pressure Science and Technology

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We applied a cubic anvil press which was originally developed for physical and geophysical research to a biological study of search for lives under very high pressure of several GPa order or more. All the animals and plants which have been tested, tardigrades at cryptobiotic state, eggs of plankton, spores of mosses and seeds of same plants, were found alive after exposure to the hydrostatic pressure of 7.5 GPa for at least 1 h. It is un-understandable that actually living creatures can withstand such a very high pressure.

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High Pressure Bioscience

Akasaka¹,

Matsuki²

2015

Subcellular Biochemistry

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The book series SUBCELLULAR BIOCHEMISTRY is a renowned and well recognized forum for disseminating advances of emerging topics in Cell Biology and related subjects. All volumes are edited by established scientists and the individual chapters are written by experts on the relevant topic. The individual chapters of each volume are fully citable and indexed in Medline/Pubmed to ensure maximum visibility of the work.

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Preserving life of moss Ptychomitrium under very high pressure

Cited by 13 publications

References 6 publications

Moss Spores Can Tolerate Ultra-high Pressure

Moss Spores Can Tolerate Ultra-high Pressure

Search for Lives under Very High Pressure and Extraordinary Strong Pressure-Tolerance of Animals and Plants

High Pressure Bioscience

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