Preserved miR-361-3p Expression Is an Independent Prognostic Indicator of Favorable Survival in Cervical Cancer

Abstract

In this study, we aimed to assess the independent prognostic value of miR-361-3p in terms of overall survival (OS) and recurrence-free survival (RFS) in cervical cancer, as well as its possible regulative network. A retrospective analysis was performed by using data from the Cancer Genome Atlas-Cervical Cancer (TCGA-CESC). Results showed that decreased miR-361-3p expression was associated with lymphovascular invasion and poor responses to primary therapy. The patients with recurrence and the deceased cases had substantially lower miR-361-3p expression compared to their respective controls. By generating Kaplan-Meier curves of OS and RFS, we found that high miR-361-3p expression was associated with better survival outcome. More importantly, univariate and multivariate analysis confirmed that high miR-361-3p expression was an independent indicator of favorable OS (HR: 0.377, 95% CI: 0.233–0.608, p < 0.001) and RFS (HR: 0.398, 95% CI: 0.192–0.825, p = 0.013). By performing bioinformatic analysis, we identified 24 genes that were negatively correlated with miR-361-3p expression. Among the potential targeting genes, SOST, MTA1, TFRC, and YAP1 are involved in some important signaling pathways modulating cervical cancer cell invasion, migration, and drug sensitivity. Therefore, it is meaningful to verify the potential regulative effect of miR-361-3p on the expression of these genes in the future.

<p>circ-MYBL2 Serves As A Sponge For miR-361-3p Promoting Cervical Cancer Cells Proliferation And Invasion</p>

“…In the present study, we found that miR-361-3p expression was significantly decreased in CC tissues and cell lines. Low miR-361-3p expression was associated with advanced FIGO stage, lymph node metastasis and poor overall survival rate in CC patients, which consistent with the previous study 27. In function assays, we showed that miR-361-3p inhibitors promoted CC cells proliferation, invasion and EMT processes.…”

“…Zhao et al suggested that miR-361-3p might function as a tumor suppressor in retinoblastoma by targeting hedgehog signaling 26. Recently, Liu et al showed that miR-361-3p expression was significantly reduced and associated with advanced clinical features and poor prognosis in CC 27. In the present study, we found that miR-361-3p expression was significantly decreased in CC tissues and cell lines.…”

Hsa_circ_0075960 Serves as a Sponge for miR-361-3p/SH2B1 in Endometrial Carcinoma

“…In addition, miR-361-3p was found to contribute to the progression of various tumors. [16][17][18][19][20] In order to identify the interaction between hsa_circ_0075960 and miR-361-3p, we mutated the possible binding sites of hsa_circ_0075960 and miR-361-3p. The luciferase assay exhibited that the miR-361-3p mimics reduced the luciferase activity of the hsa_circ_0075960 WT reporter group rather than that of the hsa_circ_0075960 MUT group, indicating that miR-361-3p bond to hsa_circ_0075960 directly ( Figure 3B).…”

Circular RNA UBAP2 contributes to tumor growth and metastasis of cervical cancer via modulating miR-361-3p/SOX4 axis

“…Many circRNAs have been found to contain diverse types and quantities of miRNAs binding sites, which allow them to specifically sponge to miRNAs, thereby repressing miRNAs activity and promoting the relative expression of corresponding target genes of the miRNAs [ 18 ], and based on this regulation, the circRNA-miRNA-mRNA function axis has been identified. Recently, miR-361-3p was identified to be decreased in CC patients and high expressed miR-361-3p was an independent, favorable indicator of long-term overall survival in CC [ 19 ]. SOX4, a member of the SOX (sex-determining region Y-related HMG box) transcription factor family, has been revealed to be malformed expressed and involved in the progression and drug-resistant of CC [ 20 ].…”