Preserved epitope‐specific T cell activation by recombinant Bet v 1‐MBP fusion proteins

Abstract

Using crude preparations of recombinant fusion proteins of Bet v 1 with MBP, multiple T cell epitopes were identified in Bet v 1. As T cell activation is preserved in this system, the generation of recombinant allergens with TH1-inducing proteins as fusion partners might be considered as a T-cell targeted approach for specific immunotherapy.

Characterization of a Hypoallergenic Recombinant Bet v 1 Variant as a Candidate for Allergen-Specific Immunotherapy

“…Our data from epitope analysis confirmed the results of other investigators, that the T cell response to Bet v 1 is highly heterogenous and that T cell epitopes are spread over the entire sequence of Bet v 1 [27,28,29,30,31]. Several different epitope specificities may occur in an individual.…”

“…The disadvantage of this approach is that T cell epitopes might be affected [26]. This is of particular importance, because T cell epitopes are spread over the entire sequence of Bet v 1 [27,28,29,30,31]. …”

Maltose-binding protein isolated from Escherichia coli induces Toll-like receptor 2-mediated viability in U937 cells

“…Upon sensing these molecules, TLRs initiate a series of downstream signalling events that drive cellular response. Before Stefan Fernandez reported that MBP potently enhanced DC maturation via TLR4 [14], MBP was considered to have a passive role in vaccine formulations [7,8]. TLR expression might induce a resistance to apoptosis and help tumour cells to escape from the immune response [27].…”

“…Meanwhile, MBP fuses with relevant proteins to improve their yield and facilitate purifi cation [6], and it confers the additional properties of enhanced stability and solubility to relevant proteins. Since the MBP tag was presumed to be inert or have minimum bioactivity [7,8], its properties were utilised to fuse with relevant proteins in various experimental subunit vaccines against pathogenic bacteria [9,10] and viruses [11,12]. However, the research reported that mice immunised with MBP developed a predominant anti-MBP IgG2a isotype profi le, produced proper levels of IFN- and low levels of IL-4, and induced the proliferation of splenocytes, compared to MBP-LiHsp83 immunised mice [13].…”

Identification of a highly promiscuous and an HLA allele‐specific T‐cell epitope in the birch major allergen Bet v 1: HLA restriction, epitope mapping and TCR sequence comparisons

“…In a previous study, we described five T-cell clones that had an identical reactivity pattern with overlapping recombinant Bet v 1 peptides [23]. Here we report a detailed characterization of the recognition of allergen-derived peptides by allergen-specific T cells by analysing cognate HLApeptide-TCR interaction of these T-cell clones.…”