PreservCyt Is an Optimal Fixative that Permits Cytologic and Molecular Analyses of Vitreoretinal Lymphoma Biopsies

Intraocular Lymphoma

“…While the authors acknowledged the diagnostic predictive power of any single clinical or multimodal imaging feature, they emphasized the importance of the combined presence of multiple suggestive features, such as abnormal sheets or veils or vitreous cells, subretinal infiltration, and a leopard spot pattern of RPE disruption -particularly in older, at-risk patients. Their work confirmed the utility of MYD88 mutation testing, [29][30][31][32][33][34][35][36][37] including the use of minimally invasive paracentesis -as reported recently by Hiemcke-Jiwa et al 38 and Miserocchi et al 39 Wang et al 15 evaluated six distinct fixatives for processing VRL fluid specimens with regard to preservation of cellular morphology in histology/immunohistochemistry and DNA/ RNA integrity. All tests were run on B-cell-like diffuse large B-cell lymphoma Pfeiffer cells.…”

“…Once diagnosed, therapeutic approaches to intraocular lymphoma vary by type, extent of involvement, treatment history, response to therapy, and treatment center. [3][4][5][6][7][8] In this issue of Ocular Immunology & Inflammation (OII), one expert consensus recommendation, 12 one invited review, 13 seven original articles [14][15][16][17][18][19][20] and four letters [21][22][23][24] address aspects of the development, diagnosis, clinical and imaging characteristics, and management of intraocular lymphomas.…”

Single B-Cell Genomic Analyses Differentiate Vitreoretinal Lymphoma from Chronic Inflammation

“…Vitreous fluid samples (volume 0.1-0.5 ml) were pre-fixed in PreservCyt ® (Hologic) as described previously 22 and underwent cytological analysis at the Singapore General Hospital (SGH), Department of Anatomical Pathology and Cytology. The vitreous samples were centrifuged at 500-800 rpm for 3 min using a Thermo Scientific Shandon J o u r n a l P r e -p r o o f Cytospin (Shandon Scientific Ltd, Cheshire, UK) and cytospin slides were obtained for Diff Quik (DQ, Baxter, McGraw Park, IL) and PAP staining using standard diagnostic and previously published IHC protocols.…”

Long-Chain Fluorescent Probe for Straightforward and Nondestructive Staining Mitochondria in Fixed Cells and Tissues

“…Primarily, in clinical environments, fixation assumes a critical role in preserving the authentic characteristics of pathological specimens to be detected . For example, in the diagnosis of vitreoretinal lymphoma, the prompt fixation of lymphoma cells from vitreous specimens becomes imperative as these cells undergo rapid degeneration within minutes . Similarly, in lung cancer screening, the expeditious fixation of squamous epithelial cells in sputum becomes essential prior to storage, transit, and staining for preserving cellular structures .…”