Preservation of vision after CaMKII-mediated protection of retinal ganglion cells

Guo, Xinzheng V.; Zhou, Jing; Starr, Christopher R.; Mohns, Ethan J.; Li, Yidong; Chen, Earnest P.; Yoon, Yong-Joong; Kellner, Christopher P.; Tanaka, Kohichi; Wang, Hongbing; Liu, Wei; Pasquale, Louis R.; Demb, Jonathan B.; Crair, Michael C.; Chen, Bin

doi:10.1016/j.cell.2021.06.031

Cited by 99 publications

(99 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… (B) RGC counting using ImageJ. The retinal whole-mount image here was recently published ( Guo et al., 2021 ). (C) Schematic diagram of data collection and analysis to obtain RGC survival results.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Unpaired t-test, ∗p < 0.0001. Original images and data were recently published ( Guo et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (B) RGC counting using ImageJ. The retinal whole-mount image here was recently published ( Guo et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… Scale bar, 40 μm. Original images were recently published ( Guo et al., 2021 ). (C) Device for intravitreal injection including the syringe, tubing, and micropipette.…”

Section: Before You Beginmentioning

confidence: 99%

“…This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021) .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Unpaired t-test, ∗p < 0.0001. Original images and data were recently published ( Guo et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (B) RGC counting using ImageJ. The retinal whole-mount image here was recently published ( Guo et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… Scale bar, 40 μm. Original images were recently published ( Guo et al., 2021 ). (C) Device for intravitreal injection including the syringe, tubing, and micropipette.…”

Section: Before You Beginmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

TET3 Contributes to Exercise‐Induced Functional Axon Regeneration and Visual Restoration

Fang,

Ji,

Shen

et al. 2024

Advanced Biology

View full text Add to dashboard Cite

Axons have intrinsically poor regenerative capacity in the mature central nervous system (CNS), leading to permanent neurological impairments in individuals. There is growing evidence that exercise is a powerful physiological intervention that can obviously enhance cell rejuvenate capacity, but its molecular mechanisms that mediate the axonal regenerative benefits remain largely unclear. Using the eye as the CNS model, here it is first indicated that placing mice in an exercise stimulation environment induced DNA methylation patterns and transcriptomes of retinal ganglion cell, promoted axon regeneration after injury, and reversed vision loss in aged mice. These beneficial effects are dependent on the DNA demethylases TET3‐mediated epigenetic effects, which increased the expression of genes associated with the regenerative growth programs, such as STAT3, Wnt5a, Klf6. Exercise training also shows with the improved mitochondrial and metabolic dysfunction in retinas and optic nerves via TET3. Collectively, these results suggested that the increased regenerative capacity induced by enhancing physical activity is mediated through epigenetic reprogramming in mouse model of optic nerve injury and in aged mouse. Understanding the molecular mechanism underlying exercise‐dependent neuronal plasticity led to the identification of novel targets for ameliorating pathologies associated with etiologically diverse diseases.

show abstract

Gold Nanostrip Array‐Mediated Wireless Electrical Stimulation for Accelerating Functional Neuronal Differentiation

Yang

Sun

et al. 2022

Advanced Science

View full text Add to dashboard Cite

Neural stem cell (NSC)‐based therapy holds great promise for the treatment of neurodegenerative diseases. Presently, however, it is hindered by poor functional neuronal differentiation. Electrical stimulation is considered one of the most effective ways to promote neuronal differentiation of NSCs. In addition to surgically implanted electrodes, traditional electrical stimulation includes wires connected to the external power supply, and an additional surgery is required to remove the electrodes or wires following stimulation, which may cause secondary injuries and infections. Herein, a novel method is reported for generation of wireless electrical signals on an Au nanostrip array by leveraging the effect of electromagnetic induction under a rotating magnetic field. The intensity of the generated electrical signals depends on the rotation speed and magnetic field strength. The Au nanostrip array‐mediated electric stimulation promotes NSC differentiation into mature neurons within 5 days, at the mRNA, protein, and function levels. The rate of differentiation is faster by at least 5 days than that in cells without treatment. The Au nanostrip array‐based wireless device also accelerates neuronal differentiation of NSCs in vivo. The novel method to accelerate the neuronal differentiation of NSCs has the advantages of wireless, timely, localized and precise controllability, and noninvasive power supplementation.

show abstract

Preservation of vision after CaMKII-mediated protection of retinal ganglion cells

Cited by 99 publications

References 84 publications

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

TET3 Contributes to Exercise‐Induced Functional Axon Regeneration and Visual Restoration

Gold Nanostrip Array‐Mediated Wireless Electrical Stimulation for Accelerating Functional Neuronal Differentiation

Contact Info

Product

Resources

About