1986
DOI: 10.1007/bf00429315
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Preservation of Thiobacillus ferroxidans and Thiobacillus thiooxidans with activity check

Abstract: Cultures of Thiobacillus ferrooxidans and Thiobacillus thiooxidans, used in biohydrometallurgical processes of economic importance, are very difficult to preserve by conventional methods. Hence, to preserve the cultures with their activity intact, various techniques were tried, after determining their respective activity in terms of Iron Oxidation Rate (IOR) and Sulfur Oxidation Rate (SOR). Among the methods tested, along with the recommended method of serial transfer in a liquid medium, were methods such as l… Show more

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Cited by 10 publications
(5 citation statements)
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“…The cells were counted by the optical density (OD) method. The OD of 0.3 at 545 nm corresponded to approximately (6.0-6.3) × 10 8 cells/ml of the culture (Gupta and Agate, 1986). This process was carried out each time after cells were harvested and obtained from this good growth of exponential phase.…”
Section: Cell Harvestmentioning
confidence: 99%
“…The cells were counted by the optical density (OD) method. The OD of 0.3 at 545 nm corresponded to approximately (6.0-6.3) × 10 8 cells/ml of the culture (Gupta and Agate, 1986). This process was carried out each time after cells were harvested and obtained from this good growth of exponential phase.…”
Section: Cell Harvestmentioning
confidence: 99%
“…A. ferrooxidans is highly susceptible to cryoinjury and the addition of a suitable protective agent is therefore critical for cell survival or for improving cell viability . In the particular case of A. ferrooxidans , protective agents such as GP (glycerate 3‐phosphate molecule), glycine betaine, skimmed milk, sucrose, sucrose + mannitol, and glycerol have already been tested in the different preservation methods . Preservation in the absence of cryoprotective agents is also possible with the aid of cryoballs, which are now widely used .…”
Section: Introductionmentioning
confidence: 99%
“…A reliable preservation method that enables long-term storage of A. ferrooxidans while maintaining cell viability would ensure the ready availability of the bacteria and prevent mutations and contamination of cultures [12][13][14][15]. Successful techniques for preserving A. ferrooxidans have already been studied [16][17][18][19]. A. ferrooxidans is highly susceptible to cryoinjury [17] and the addition of a suitable protective agent is therefore critical for cell survival or for improving cell viability [17,[19][20][21].…”
mentioning
confidence: 99%
“…Maintenance: Mineral or elementary sulfur oxidizing strains are maintained quite easily following growth in 9K or GEBS under air using 5 g/L sterile pyrite, chalcopyrite, lignite or roll sulfur as the electron donor. After growth is evident, viability is retained for 2-4 months at 4-10 °C (Gupta and Agate, 1986). Many Acidithiobacillus spp.…”
mentioning
confidence: 99%
“…Lyophilization of Acidithiobacillus spp. is not usually very successful (Gupta and Agate, 1986). Enrichment and isolation: For solid samples, 1-2 g soil, corroded concrete, coal or minerals are added directly to 50 mL of the appropriate basal salts, supplemented with the required electron donor in a sterile, wide-mouth Erlenmeyer flask.…”
mentioning
confidence: 99%