Preservation of freeze-dried liposomes by trehalose

Crowe, Lois M.; Crowe, John H.; Rudolph, Alan S.; Womersley, Christopher; Appel, Lori

doi:10.1016/0003-9861(85)90498-9

Cited by 326 publications

(177 citation statements)

References 19 publications

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“…The changes in T m that were observed when using trehalose, which is one of the most effective lyoprotectants 21,22 , were similar to those observed with SB n . It has been reported that the addition of trehalose decreases the T m of the freeze-dried liposomes 25 for dried DPPC liposomes 26 .…”

Section: Effect Of Sb N On the Phase Transitions Of Hydrated Dppc Lipsupporting

confidence: 63%

“…CF retention in the presence of SB n 10 is slightly but significantly larger compared with that in the presence of trehalose, an additive known to exert a significant lyoprotective effect 20,21 .…”

Section: Structural Integrity Of Dppc Liposomes After Fd/rhmentioning

confidence: 96%

“…To date, it has been demonstrated that some types of lyoprotectants, such as amino acids 16,17 , carbohydrates 18 e.g. glucose 19 , trehalose 20,21 , arbutine 22 and glycerol 23 could exert a lyoprotective effect. In particular, trehalose could work effectively as a lyoprotectant.…”

mentioning

confidence: 99%

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Lyoprotective Effect of Alkyl Sulfobetaines for Freeze-drying 1,2-Dipalmitoyl-<i>sn</i>-glycero-3-phosphocholine Liposomes

et al. 2017

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Section: Effect Of Sb N On the Phase Transitions Of Hydrated Dppc Lipsupporting

confidence: 63%

Section: Structural Integrity Of Dppc Liposomes After Fd/rhmentioning

confidence: 96%

See 1 more Smart Citation

Lyoprotective Effect of Alkyl Sulfobetaines for Freeze-drying 1,2-Dipalmitoyl-<i>sn</i>-glycero-3-phosphocholine Liposomes

et al. 2017

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“…Another possibility is that trehalose could replace the water and cause stabilization due to the formation of hydrogen bridges with components of the cells when the water is removed [28]. Therefore, trehalose has been successfully used to protect liposomes [29], bacteria [30], yeast [31], retroviruses [32], proteins [33], chromatin [34], and cell lines in vitro [35], [36] and [37].…”

Section: Discussionmentioning

confidence: 99%

Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

et al. 2007

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Freeze-drying sperm is an alternative to cryopreservation. Although sperm from various species has been freeze-dried, there are few reports for bovine sperm. The primary objective of this study was to evaluate the protective effect of various freeze-drying media on the structural and functional components of bovine sperm. The media tested were composed of TCM 199 with Hanks salts supplemented with 10% fetal calf serum (FCS) and TCM 199 with Hanks salts supplemented with 10% FCS and 0.2 M trehalose and EGTA solution. The efficiency of each medium on the preservation of freeze-dried sperm structures was evaluated with conventional and electron microscopy, DNA integrity was analyzed by a TUNEL assay, and fertilizing ability of lyophilized sperm was determined with ICSI. Although the plasma membrane was damaged in all media tested, mitochondria were similarly preserved in all freeze-drying treatments. The acrosome was best preserved in the media that contained trehalose (other treatments also conserved this structure). In contrast, media containing EGTA or trehalose most effectively preserved the nuclei in freeze-dried sperm, with only 2 and 5%, respectively, of cells with fragmented DNA. Furthermore, sperm conserved with these media also had higher (P < 0.05) rates of sperm head decondensation (32.5 and 27.5%), pronucleus formation (37.5 and 45.0%) and blastocyst formation (19.4 and 18.3%) than medium supplemented with FCS (15.0, 20.0 and 10.2%, respectively). In conclusion, media with EGTA and trehalose adequately protected bovine sperm during freeze-drying by preserving the viability of their nuclei.

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“…LUVs and SUVs, however, have higher free energies and some energy must be dissipated into the system in order to produce them. Exceptions are charged phospholipids which also form some uni-and oligo-lamellar structures and some double-chained surfactants which form spontaneously stable vesicles (Talmon et al, 1983 (Crowe et al, 1985). This phenomenon is probably due to the specific interaction of sugars with polar heads and can be very useful for the long-term storage of vesicles (Strauss & Hauser, 1986;Crowe et al, 1987).…”

Section: Preparation Methodsmentioning

confidence: 99%