1986
DOI: 10.1007/bf00251061
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Preservation of cellular polarity in isolated hepatocytes

Abstract: The distribution of actin, myosin and tropomyosin in freshly isolated and short-term cultured rat hepatocytes was investigated by use of both rhodaminyl-phalloidin staining and immunofluorescence techniques. The cytoskeletal proteins were mainly located in distinct areas of the hepatocyte membrane, corresponding to their accumulation in the bile-canalicular region of liver tissue. In freshly prepared cells, these sections resembled sharp, angled or branched bands, similar to the pattern of hemicanaliculi. Duri… Show more

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Cited by 17 publications
(1 citation statement)
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“…Unlike single cells, hepatocyte couplets retain their apical polarity with tight junctions dividing the canalicular and basolateral domains. The mitochondria, smooth and rough endoplasmic reticulum and Golgi apparatus of these couplets retain normal ultrastructural appearances (Gautam et al, 1987;Nikola and Frimmer, 1986). Functional gap junctions (Reverdin and Weingart, 1988;Spray et al, 1986) as well as tight junctions (Gautum et al, 1987) are maintained between the adjacent cells.…”
Section: Introductionmentioning
confidence: 99%
“…Unlike single cells, hepatocyte couplets retain their apical polarity with tight junctions dividing the canalicular and basolateral domains. The mitochondria, smooth and rough endoplasmic reticulum and Golgi apparatus of these couplets retain normal ultrastructural appearances (Gautam et al, 1987;Nikola and Frimmer, 1986). Functional gap junctions (Reverdin and Weingart, 1988;Spray et al, 1986) as well as tight junctions (Gautum et al, 1987) are maintained between the adjacent cells.…”
Section: Introductionmentioning
confidence: 99%