Preservation and Distribution of Fungal Cultures

Nakasone, Karen K.; Peterson, Stephen W.; Jong, S. C.

doi:10.1016/b978-012509551-8/50006-4

Cited by 88 publications

(82 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, drying is a useful method of preservation for cultures that produce spores or other structures (FONG et al, 2000, NAKASONE et al, 2004. Some fungi can preserved easily and successfully for many years in dry, sterile soil or sand.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the gelatin technique for the preservation of phytopathogenic fungi

Beloti¹,

Juliatti²,

Juliatti³

2017

Biosci. J.

View full text Add to dashboard Cite

ABSTRACT:The preservation methods for fungi have great importance in ex situ collections, representing important biological heritage, useful for mycologists and plant pathologists in several scientific works. However, there is a lack of studies for a suiTable and efficient preservation method for the different groups of fungi. Although, the most appropriate is the one that maintain, even after long periods, the original characteristics of culture: viability, sporulation and pathogenicity, excluding mutations and undesirable contamination. The choice will depend of the laboratory infrastructure, microorganism, objectives, preferences and knowledge of the researcher. We conducted this study inside the Laboratory of Mycology and Plant Protection (LAMIP) in UFU (Universidade Federal de Uberlândia), localized in Uberlândia (MG), Brazil. The objective was to evaluate the gelatin preservation method (17 cultures), never used before for phytopathogenic fungi. Other classical methods were concomitantly evaluated, such as sterile soil (68 cultures), resistant structures (Sclerotinia sclerotiorum) in 4°C (10 strains) and mineral oil (31 cultures). We examined the time for maintaining the viability, sporulation and colonization in host tissues preserved in different dates. The gelatin method remained viability in 10 cultures; this method is suiTable for preservation of the genera and species: Colletotrichum spp., Septoria spp., Fusarium spp., F. moniliforme var. subglutinans, Macrophomina spp., Phomopsis spp. and Verticillium spp. The viability remained in 38 strains of sterile soil, three of mineral oil, and one strain of sclerotia reached a maximum preservation time in 4°C of four years.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of the gelatin technique for the preservation of phytopathogenic fungi

Beloti¹,

Juliatti²,

Juliatti³

2017

Biosci. J.

View full text Add to dashboard Cite

show abstract

“…Kitamoto et al (2002) grew cultures over SDM (65% moisture W/W) with 10% glycerol as the cryoprotectant and then rapidly froze them at 285uC and tested viability by inoculating on PDA; several zygomycete cultures remained viable for 20 mo. These and other methods are discussed in detail by Fennell (1960), Jong and Birmingham (2001), and Nakasone et al (2004).…”

Section: Maintenancementioning

confidence: 99%

Methods Used by Dr. R. K. Benjamin, and Other Mycologists, to Isolate Zygomycetes

Benny¹

2008

aliso

View full text Add to dashboard Cite

The methods that Dr. Richard K. Benjamin used to isolate Zygomycetes are discussed. These processes involved the following five steps: (1) collection, (2) plating, (3) isolation, (4) culture, and (5) maintenance. Additional methods, materials and modifications used to isolate Zygomycetes are summarized. The author considers the flattening of the aerial hyphae onto the substrate of the faster-and higher-growing Mucorales for several consecutive days to be the critical step in isolating species of Coemansia, Piptocephalis, Syncephalis, and Dimargaritales. The methods used by other scholars to isolate, culture, and study many taxa in Zygomycetes also are discussed.

show abstract

“…4.6. PDA Slants: The preservation of fungal cultures on agar slants is a widely used classical method for preservation of fungi and in this method cultures are generally stored between 4-20˚C [55,68].…”

Section: 5sterile Distilled Watermentioning

confidence: 99%

Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials

Tidke¹,

Kl²,

Kiran³

et al. 2017

IOSR JBB

View full text Add to dashboard Cite

Preservation and Distribution of Fungal Cultures

Cited by 88 publications

References 0 publications

Evaluation of the gelatin technique for the preservation of phytopathogenic fungi

Evaluation of the gelatin technique for the preservation of phytopathogenic fungi

Methods Used by Dr. R. K. Benjamin, and Other Mycologists, to Isolate Zygomycetes

Current Understanding of Endophytes: Their Relevance, Importance, and Industrial Potentials

Contact Info

Product

Resources

About