Presentation of functional foreign peptides on the surface of SV40 virus-like particles

Takahashi, Ryou U.; Kanesashi, Shin nosuke; Inoue, Takamitsu; Enomoto, Teruya; Kawano, Masaaki; Tsukamoto, Hiroko; Takeshita, Fumitaka; Imai, Takeshi; Ochiya, Takahiro; Kataoka, Kohsuke; Yamaguchi, Yuki; Handa, Hiroshi

doi:10.1016/j.jbiotec.2008.05.012

Cited by 35 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RGD is a targeting peptide that is frequently used due to its high affinity for α v β 3 and α v β 5 integrins, which are involved in angiogenesis and associated with cancer proliferation. 81, 151, 182, 183 The association of RGD-targeted particles with tumor vascular endothelium has been demonstrated in mice, although the study also indicated that better tumor localization would be achieved with greater circulation time imparted through incorporation of a better shielding linker (see Section 4.1.1 ). 151 CPMV displaying peptide F56, which was discovered through phage display, has been used to target vascular endothelial growth fa ctor receptor-1 (VEGFR-1), with accumulation throughout the tumor observed compared to no detectable uptake of non-targeted particles.…”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

View full text Add to dashboard Cite

Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

show abstract

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

View full text Add to dashboard Cite

show abstract

“…The purified capsids were then dialyzed in a buffer containing 20 mM Tris (pH 7.9) and 50 mM NaCl and observed under the electron microscope. Wild type SV40 virion was produced by the transfection of recircularized DNA excised from pUC-SV40 by BamHI digestion as described previously (28). It was then amplified in CV-1 cells and purified using the same method used for the Sf-9-derived VP1 capsid, as described above with minor modifications.…”

Section: Methodsmentioning

confidence: 99%

Calcium Bridge Triggers Capsid Disassembly in the Cell Entry Process of Simian Virus 40

Kawano

Tsukamoto

et al. 2009

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

The calcium bridge between the pentamers of polyoma viruses maintains capsid metastability. It has been shown that viral infection is profoundly inhibited by the substitution of lysine for glutamate in one calcium-binding residue of the SV40 capsid protein, VP1. However, it is unclear how the calcium bridge affects SV40 infectivity. In this in vitro study, we analyzed the influence of host cell components on SV40 capsid stability. We used an SV40 mutant capsid (E330K) in which lysine had been substituted for glutamate 330 in protein VP1. The mutant capsid retained the ability to interact with the SV40 cellular receptor GM1, and the internalized mutant capsid accumulated in caveolin-1-mediated endocytic vesicles and was then translocated to the endoplasmic reticulum (ER) region. However, when placed in ER-rich microsome, the mutant capsid retained its spherical structure in contrast to the wild type, which disassembled. Structural analysis of the mutant capsid with cryo-electron microscopy and image reconstruction revealed altered pentamer coordination, possibly as a result of electrostatic interaction, although its overall structure resembled that of the wild type. These results indicate that the calcium ion serves as a trigger at the pentamer interface, which switches on capsid disassembly, and that the failure of the E330K mutant capsid to disassemble is attributable to an inadequate triggering system. Our data also indicate that calcium depletion-induced SV40 capsid disassembly may occur in the ER region and that this is essential for successful SV40 infection.Viruses use their capsid to transmit genome from cell to cell and thus gain access to cellular synthesis machinery. To achieve this, the capsids of non-enveloped viruses perform a myriad of tasks during viral infection, such as viral genome encapsidation, binding to host cell surface receptors, selection of the appropriate entry route, and disassembly to release the genome into a suitable replication environment. The viral capsid must be robust enough to protect the genome against a harsh extracellular environment while being able to disassemble easily within the cell in order to release its genome. Viruses have therefore evolved various strategies to balance the stability of the capsid against its capacity to disassemble. The switch between these states is triggered by the cell.SV40, a polyomavirus, is composed of a circular doublestranded DNA genome enclosed within an icosahedral capsid. This virus was discovered in the early 1960s as a frequent contaminant of the rhesus macaque kidney cell cultures used to grow poliomyelitis vaccines (1-3). SV40 induces tumors in hamsters and has been used extensively as a model for tumorcausing viruses (2). SV40 capsid is composed of three structural proteins: VP1, VP2, and VP3. The major structural protein, VP1, is arranged in 72 pentameric rings that are assembled into an icosahedral T ϭ 7d surface lattice (4 -6). The two minor structural proteins, VP2 and VP3, are located on the inner surface of the capsid in a r...

show abstract

“…VLPs are excellent systems for in vitro drug delivery. Although in vivo applications of VLPs are not currently wide spread, several studies aiming to make their application possible are already in process [63]. Unfortunately many viruses infect more than one cell type.…”

Section: Vlps As Molecular Delivery Tools and Vaccinesmentioning

confidence: 99%

“…Other interesting model is the simian virus 40 (SV40), whose VLPs can be an efficient gene delivery vehicle that infect a broad range of cells and tissues. Takahashi and coworkers [63] identified two sites within the surface loops of SV40 Vp1 that can display foreign peptides without interfering with the assembly of VLPs.…”

Section: Vlps As Molecular Delivery Tools and Vaccinesmentioning

confidence: 99%

Advances in the Development of Virus-Like Particles as Tools in Medicine and Nanoscience

Sánchez-Rodríguez¹,

Munch-Anguiano²,

Bustos‐Jaimes³

2010

curr chem biol

View full text Add to dashboard Cite

Virus-like particles (VLPs) are viral coats formed by the self-assembly of the components of viruses excluding their genetic material. These particles may be used as vectors to transport molecules chemically attached on the VLP surface, or as vessels for the systemic delivery of pharmacologically important molecules as drugs, siRNAs, genes, mRNAs, magnetic nanoparticles, quantum dots and other medical-imaging aids. Herein we review the recent advances in heterologous production and purification of VLPs, the current trends in chemical modification of VLPs, and the advances on the application of VLPs as vectors, tools for medical imaging and materials for nanotechnology.

show abstract

Presentation of functional foreign peptides on the surface of SV40 virus-like particles

Cited by 35 publications

References 34 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Calcium Bridge Triggers Capsid Disassembly in the Cell Entry Process of Simian Virus 40

Advances in the Development of Virus-Like Particles as Tools in Medicine and Nanoscience

Contact Info

Product

Resources

About