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Kadri, Aïssa; Miranda, Adelina

doi:10.3917/naqd.028.0007

Cited by 2 publications

(3 citation statements)

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“…The region of interest (ROI) was first segmented out of the captured images before analyzing the results and plotting the DEP spectra of the respective cell populations. Instead of using a circular band to segment the circular area of the dot as conducted by Fatoyinbo et al (2008), Kadri (2010) produced good DEP spectra by segmenting the dot area using a square with a width of half of the radius of the dot [24,25]. Therefore, a square with a width of 100 m was used in this project to segment the central part of the dot, as illustrated schematically in Fig.…”

Section: Image Processingmentioning

confidence: 99%

“…The best ROI to construct the Beer-Lambert area for the Cumulative Modal Intensity Shift method is the central part of the dot [24]. Moreover, a square with a width of half the radius of the dot was proved to produce good DEP spectra compared with a circular ROI [25]. Therefore, a 100 m square was used to segment the central part of the dots in each captured image to form the ROI, as highlighted in Fig.…”

Section: Dep Experimentsmentioning

confidence: 99%

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Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

et al. 2015

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Dielectrophoresis (DEP), the induced movement of dielectric particles placed in a nonuniform electric field, has been used as a potential technique for manipulation and separation of many biological samples without destructive consequences to the cell. Cells of the same genotype in different physiological and pathological states have unique morphological and structural features, therefore, it is possible to differentiate between them using their DEP responses. This paper reports the experimental discrimination of normal and dengue-infected human hepatic fetal epithelial cells (WRL-68 cells) based on their DEP crossover frequency, at which no resultant movement occurs in the cells in response to the DEP force. A microarray dot electrode was used to conduct the DEP experiments. The DEP forces applied to the cells were quantified by analyzing the light intensity shift within the electrode's dot region based on the Cumulative Modal Intensity Shift image analysis technique. The differences in dielectric properties between infected and uninfected cells were exploited by plotting a unique DEP spectrum for each set of cells. We observed that the crossover frequency decreased from 220 kHz for the normal WRL-68 cells to 140 kHz after infection with the dengue virus in a medium conductivity of 100 μS/cm. We conclude that the change in the DEP crossover frequency between dengue-infected cells and their healthy counterparts should allow direct characterization of these cell types by exploiting their electrophysiological properties.

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Section: Image Processingmentioning

confidence: 99%

Section: Dep Experimentsmentioning

confidence: 99%

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

et al. 2015

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“…In order to promote proliferation of ASCs prior to 3D chondrogenic differentiation, fetal calf serum (FCS) is usually used. However, FCS implies various risks, such as internalization of xenogeneic proteins (Spees et al, 2004), the risk of virus and prion transmission and immunological risks (Kadri et al, 2007;Tuschong et al, 2002). Thus, the use of FCS for human products is not recommended by European legislation (Doucet et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Human platelet lysate successfully promotes proliferation and subsequent chondrogenic differentiation of adipose-derived stem cells: a comparison with articular chondrocytes

Hildner

Eder

Hofer

et al. 2013

J Tissue Eng Regen Med

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Fetal calf serum (FCS) bears a potential risk for carrying diseases and eliciting immune reactions. Nevertheless, it still represents the gold standard as medium supplement in cell culture. In the present study, human platelet lysate (PL) was tested as an alternative to FCS for the expansion and subsequent chondrogenic differentiation of human adipose-derived stem cells (ASCs). ASCs were expanded with 10% FCS (group F) or 5% PL (group P). Subsequently, three-dimensional (3D) micromass pellets were created and cultured for 5 weeks in chondrogenic differentiation medium. Additionally, the de- and redifferentiation potential of human articular chondrocytes (HACs) was evaluated and compared to ASCs. Both HACs and ASCs cultured with PL showed strongly enhanced proliferation rates. Redifferentiation of HACs was possible for cells expanded up to 3.3 population doublings (PD). At this stage, PL-expanded HACs demonstrated better redifferentiation potential than FCS-expanded cells. ASCs could also be differentiated following extended passaging. Glycosaminoglycan (GAG) quantification and qRT-PCR of 10 cartilage related markers demonstrated a tendency for increased chondrogenic differentiation of PL-expanded ASCs compared to cells expanded with FCS. Histologically, collagen type II but also collagen type X was mainly present in group P. The present study demonstrates that PL strongly induces proliferation of ASCs, while the chondrogenic differentiation potential is retained. HACs also showed enhanced proliferation and even better redifferentiation when previously expanded with PL. This suggests that PL is superior to FCS as a supplement for the expansion of ASCs and HACs, particularly with regard to chondrogenic (re)differentiation.

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Cited by 2 publications

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Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

Discriminating dengue‐infected hepatic cells (WRL‐68) using dielectrophoresis

Human platelet lysate successfully promotes proliferation and subsequent chondrogenic differentiation of adipose-derived stem cells: a comparison with articular chondrocytes

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