Presence of Sarcoma Genome in a “Non-infectious” Mammalian Virus

Gazdar, Adi F.; Phillips, Leo A.; Sarma, Padman S.; Peebles, Paul T.; Chopra, Harish C.

doi:10.1038/newbio234069a0

Cited by 41 publications

(15 citation statements)

References 23 publications

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“…The Gazdar murine sarcoma virus particles, which are morphologically immature and possess a precursor gag polyprotein, but not mature gag protein [34], can have a p 65 protein which be cleaved in vitro by MuLV proteolytic activity [43]. These results indicate that the proviral HIV-1 genome in M 10/LAV-2 cells might be mutated in the HIV-1 protease region as in HTG-2 cells which continuously produced Gazdar murine sarcoma virus [4].…”

Section: Discussionmentioning

confidence: 80%

The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

Goto

Ikuta

Zhang

et al. 1990

Archives of Virology

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Three cell clones producing large numbers of infectious or noninfectious particles of human immunodeficiency virus type 1 (HIV-1), designated M 10/LAV-2, M 16/LAV-3, and MT/LAV-17, were isolated from persistently HIV-1-infected MT-4 cells. In M 10/LAV-2, the HIV-1 proteins were defective in the cleavage of gag precursor protein, and the particles were doughnut-shaped with a double-ring structure. These particles were produced by budding at the cell surface from crescentic structures followed by the formation of double-ring structures. The viral proteins in M 16/LAV-3 were defective in the cleavage of env precursor protein. The morphology of the virus particles was intact, and an electron dense bar-shaped core was seen inside a single-ring enveloped structure. The intact particles were released from the cell surface by a budding process in which crescent shape structures first appeared at the cell membrane, then subsequently just before release matured to a complete structure with an electron dense core. In MT/LAV-17, the synthesis of HIV-1 proteins was normal, and the particles were teardrop-shaped with an intact core structure. These particles were produced by budding with an electron dense core at the cell surface. Thus, it was suggested that the morphological maturation of HIV-1 particles was completed just before release from the cell surface in several cell clones producing HIV-1 particles of different morphology.

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Section: Discussionmentioning

confidence: 80%

The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

Goto

Ikuta

Zhang

et al. 1990

Archives of Virology

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“…The sera of tumorbearing hamsters containing C F antibodies satisfactorily served for the C F detection of the murine leukemia gs viral antigens as well as infectious virus by the COMUL test. This induction of gs antigen and corresponding antibodies in hamsters appears to be largely due to the presence in the hamster tumors and cultures derived from such tumors of a noninfectious C-type virus with the properties of a sarcoma virus (25).…”

mentioning

confidence: 96%

“…Partially purified concentrates (6,26) of primary and transplanted hamster tumors were free of demonstrable infectious MSV or MuLV as tested by inoculation into newborn BALB/c and NIH Swiss mice and by COMUL tests in vitro in cultures of NIH Swiss MEF and Syrian hamster embryo fibroblasts (4, 1 1 ) . Electron microscopic examination of primary and transplanted hamster tumors and tissue culture cell lines (HTG1 and HTG2) derived from transplanted tumors showed mature and budding C-type virus particles (22,25).…”

mentioning

confidence: 99%

Gazdar Strain of Murine Sarcoma Virus. Biologic and Antigenic Interactions with the Heterologous Hamster Host

Sarma¹,

Gazdar²,

Turner³

et al. 1972

Experimental Biology and Medicine

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“…Gz-MuSV was found to be closely related to the ml isolate of Moloney MuSV (Mo-MuSV) (Donoghue et aL, 1979a). Gazdar MuSV is similar to Mo-MuSV in that it is a replication-defective virus and has acquired the 1.2 kb mouse mos cell sequence (Donoghue et al, 1979a, b;Gazdar et al, 1971). One particular line of hamster cells (HTG2) infected with Gz-MuSV produces a non-infectious retrovirus with immature morphology (Gazdar et al, 1972a, b).…”

Section: Introductionmentioning

confidence: 99%

Further Characterization of the in vitro Products Generated by Proteolytic Cleavage of Gazdar Murine Sarcoma Virus p65gag

Maxwell

Arlinghaus

1985

Journal of General Virology

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SUMMARYIn vitro proteolytic cleavage of the Gazdar murine sarcoma virus (Gz-MuSV) p65 gag polypeptide (Gz-p65gag) was facilitated by detergent-disrupted Moloney murine leukaemia virus (Mo-MuLV). Incubation of radioactively labelled Gz-p65 g~g in the presence of unlabelled Mo-MuLV under optimal conditions resulted in the cleavage of Gz-p65 o~g to proteins of 40 000 (P40) and 25 000 (P25) M r. P40 and P25 appeared to be similar in both mobility and antigenicity to Mo-MuLV intermediates, Pr40 g~g and Pr25 gag, previously found in infected cells. Additional proteins of 30000 (Gz-p30), 15000 (Gz-pl2), 12000 (Gz-pl5) and 10000 (Gzpl0) Mr were also generated upon cleavage of Gz-p65 gag and contained antigenic determinants of Mo-MuLV structural proteins p30, ppl2, p15 and pl0, respectively. Both detergent-disrupted Mo-MuLV and Rauscher murine leukaemia virus produced similar cleavage profiles. Trypsin and detergent-disrupted mouse mammary tumour virus generated cleavage patterns very different from that produced by Mo-MuLV. Both visual and quantitative time studies of the reaction indicated that P40 gave rise to Gz-p30 and Gz-pl0. Tryptic peptide mapping of Gz-p65 gag and its cleavage products supported the results obtained from both immunoprecipitation studies with anti-gag sera and the kinetics of cleavage of Gz-p65 g~g. Both Mo-MuLV Pr65 gag and Gz-p65 gag were found to be very similar in primary sequence as judged by peptide mapping. P40 produced tryptic peptides that comigrated with Mo-MuLV p30 peptides; P25 contained tryptic peptides that were also found in Mo-MuLV p15. Gz-p30 and Gz-pl5 contained the tryptic peptides of MoMuLV p30 and pl 5, respectively, that were found in P40 and P25. The Gz-p 10 fraction contained a tryptic peptide that was also found in P40, but not p30. These results provide good evidence that the protease packaged within Mo-MuLV can cleave, in vitro, the gag-related polyprotein of Gz-MuSV in a manner very similar to the processing of Mo-MuLV Pr65O~9 in infected cell culture systems.

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Presence of Sarcoma Genome in a “Non-infectious” Mammalian Virus

Cited by 41 publications

References 23 publications

The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

Gazdar Strain of Murine Sarcoma Virus. Biologic and Antigenic Interactions with the Heterologous Hamster Host

Further Characterization of the in vitro Products Generated by Proteolytic Cleavage of Gazdar Murine Sarcoma Virus p65gag

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