Presence of nitric oxide synthase activity in roots and nodules of <i>Lupinus albus</i>

Cueto, Mercedes; Hernández‐Perera, Octavio; Martı́n, Raquel; Bentura, M. L.; Rodrigo, José; Lamas, Santiago; Golvano, Maria Pilar

doi:10.1016/s0014-5793(96)01232-x

Cited by 284 publications

(178 citation statements)

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“…However, after entering the plant, without being assimilated, NO might play a role similar to that of NO synthesized by nitric oxide synthase after degradation of arginine. An indication of the presence of nitric oxide synthase in plants was found by Cueto et al (1996). Although the role of NO in plants is still largely unknown, it might act as a signal that in small amounts initiates biochemical reactions such as the oxidation of unsaturated lipids shown in a lipid model system (Lai et al, 1996).…”

Section: Assimilation Of Gaseous Nitrogenous Pollutantsmentioning

confidence: 99%

Impact of gaseous nitrogen deposition on plant functioning

et al. 1998

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 Dry deposition of NH$ and NO x (NO and NO # ) can affect plant metabolism at the cellular and whole-plant level. Gaseous pollutants enter the plant mainly through the stomata, and once in the apoplast NH $ dissolves to form NH % + , whereas NO # dissolves to form NO $ − and NO # − . The latter compound can also be formed after exposure to NO. There is evidence that NH $ -N and NO x -N can be reversibly stored in the apoplast. Temporary storage might affect processes such as absorption rate, assimilation and re-emission. Once formed, NO $ − and NO # − can be reduced, and NH % + can be assimilated via the normal enzymatic pathways, nitrate reductase (NR), nitrite reductase and the glutamine synthetase\glutamate synthase (GS\GOGAT) cycle. Fumigation with low concentrations of atmospheric NH $ increases in vitro glutamine synthetase activity, but whether this involves both or only one of the GS isoforms is still an open question. There seems to be no correlation between fumigation with low concentrations of NH $ and in vitro GDH activity. The contribution of atmospheric NH $ and NO # deposition to the N budget of the whole plant has been calculated for various atmospheric pollutant concentrations and relative growth rates (s). It is concluded that at current ambient atmospheric N concentrations the direct impact of gaseous N uptake by foliage on plant growth is generally small. Key words : Apoplastic storage, gaseous nitrogen deposition, glutamate dehydrogenase, glutamine synthetase, plant nitrogen demand, sulphur dioxide root\shoot interaction, ammonia, nitrogen oxides. Anthropogenic emissions of nitrogen-containing air pollutants far exceed natural emissions in Europe and North America. These emissions cause two types of effect : generation of tropospheric O $ , and excess N deposition, which can result in direct phytotoxic effects, eutrophication, acidification and stimulation of greenhouse gas production. Anthropogenic emissions of N-containing air pollutants have resulted in atmospheric N deposition 5-20 times higher than in natural conditions. Indications of N excess in the field can be seen from : (1) changes in the species composition of indigenous vegetation types, with an increase in nitrophilous species and a decrease in others, and (2) increases in foliar N * To whom correspondence should be addressed. E-mail : g.stulen!biol.rug.nl. Abbreviations : GDH, glutamate dehydrogenase ; GS, glutamine synthetase ; GOGAT, glutamate synthase ; , net nitrate uptake rate ; NR, nitrate reductase ; P95, 95 percentile ; , plant nitrogen content ; , relative growth rate.

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Section: Assimilation Of Gaseous Nitrogenous Pollutantsmentioning

confidence: 99%

Impact of gaseous nitrogen deposition on plant functioning

et al. 1998

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“…Since one of the most accepted functions for peroxidase is its role in the lignification of xylem vessels (Nose et al 1995;Otter & Polle 1997;Ros Barceló 1995, 1997, and since a NO synthase-like activity has been putatively localized in the vascular bundles of the root of Lupinus albus (Cueto et al 1996), we have studied the effect of some NO-releasing compounds, and of NO itself, on xylem peroxidase from Zinnia elegans. In addition, the effect of NO-releasing compounds on H 2 O 2 production by the lignifying xylem was also studied.…”

Section: Introductionmentioning

confidence: 99%

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Ferrer

Barceló

1999

Plant Cell & Environment

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Sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) are two nitric oxide (NO)-releasing compounds that, when used at 5·0 mol m -3 concentrations, are capable of releasing NO in the aqueous phase at a rate of 35 ± 4 and 47 ± 5 µmol m -3 s -1 , respectively. For this reason, the effect of SNP and SNAP on coniferyl alcohol peroxidase and on H 2 O 2 production by the lignifying xylem of Zinnia elegans (L.) has been studied in order to ascertain whether NO, which is a synchronizing chemical messenger in animals and an air pollutant, has any effect on these plant-specific metabolic aspects. The results showed that both SNP and SNAP provoke an inhibition in the mol m -3 concentration range of the coniferyl alcohol peroxidase activity of a basic peroxidase isoenzyme present in the intercellular washing fluid of Z. elegans. The effect of these NO-releasing compounds on peroxidase was confirmed through histochemical studies, which showed that xylem peroxidase was totally inhibited by treatment with these NO donors at 5·0 mol m -3 , and by NO at a concentration change rate of 55 ± 5 and 110 ± 9 µmol m -3 s -1 . However, SNP, at 5·0 mol m -3 , does not have any effect on H 2 O 2 production by the xylem of Z. elegans. The fact that SNP and SNAP are two structurally dissimilar compounds which only share the common ability to release NO in aqueous buffer, and that similar results were obtained when using NO itself, suggest that NO could be considered as an inhibitor of coniferyl alcohol peroxidase which does not affect H 2 O 2 production in the xylem of Z. elegans.Key-words: Zinnia elegans; coniferyl alcohol oxidation; H 2 O 2 production; inhibition; nitric oxide; peroxidase; xylem.Abbreviations: IWF, intercellular washing fluid; pCMB, pchloromercuribenzoic acid; SNAP, S-nitroso-N-acetyl-penicillamine; SNP, sodium nitroprusside; TMB, 3,3',5,5'-tetramethylbenzidine. INTRODUCTIONNitric oxide (NO) is a paramagnetic relatively stable freeradical molecule involved in many physiological processes in animals, where it serves as a synchronizing chemical messenger involved in vasorelaxation, platelet inhibition, neurotransmission, cytotoxicity and immunoregulation (Anbar 1995). In animal cells, NO is synthesized by the enzyme NO synthase (Knowles 1997), and most of its biological regulatory properties have been explained on the basis of its capacity to act as an iron ligand in haemoproteins (Stamler, Singel & Loscalzo 1992). Dual (activating or inhibitory) effects of NO on haemoproteins have been described (Tsai 1994), and the nature of the effect has been suggested to depend to a great extent on the resting state (oxidation state) of the haemoprotein, which conditions the ligand properties and the electronic configuration of the haem iron (Tsai 1994). In the case of catalase, it has been shown that NO reversibly binds to the haem of catalase and inhibits H 2 O 2 breakdown with a K i of about 0·2 mmol m -3 (Brown et al. 1997).Evidence is emerging to support the possibility that NO may also act as chemical mess...

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“…; García-Mata and Lamattina, 2001), high temperature in Lucerne, Switzerland, low temperature in tomato (Solanum lycopersicum), wheat, and maize (Cueto et al, 1996); Al toxicity in rice bean (Vigna umbellata; Zhou et al, 2012); Fe deficiency in Arabidopsis (Chen et al, 2010b); and P deficiency in white lupin (Lupinus albus; Meng et al, 2012). NO improves the growth of white lupin under P deficiency through inducing cluster-root development and citrate exudation .…”

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confidence: 99%

Differential effects of nitrogen forms on cell wall phosphorus remobilization in rice (Oryza sativa) are mediated by nitric oxide, pectin content and the expression of the phosphate transporter OsPT2

Zhu¹,

Zhu²,

Hu³

et al. 2016

Plant Physiol.

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+ is a major source of inorganic nitrogen for rice (Oryza sativa), and NH 4 + is known to stimulate the uptake of phosphorus (P). However, it is unclear whether NH 4 + can also stimulate P remobilization when rice is grown under P-deficient conditions. In this study, we use the two rice cultivars 'Nipponbare' and 'Kasalath' that differ in their cell wall P reutilization, to demonstrate that NH 4 + positively regulates the pectin content and activity of pectin methylesterase in root cell walls under 2P conditions, thereby remobilizing more P from the cell wall and increasing soluble P in roots and shoots. Interestingly, our results show that more NO (nitric oxide) was produced in the rice root when NH 4 + was applied as the sole nitrogen source compared with the NO 3 2. The effect of NO on the reutilization of P from the cell walls was further demonstrated through the application of the NO donor SNP (sodium nitroprusside) and c-PTIO (NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide). What's more, the P-transporter gene OsPT2 is up-regulated under NH 4 + supplementation and is therefore involved in the stimulated P remobilization. In conclusion, our data provide novel (to our knowledge) insight into the regulatory mechanism by which NH 4 + stimulates Pi reutilization in cell walls of rice.

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Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus

Abstract: NO is a widespread messenger molecule in physiology. We were interested in investigating whether an NOgenerating system could be present in plants. NO

Cited by 284 publications

References 30 publications

Impact of gaseous nitrogen deposition on plant functioning

Impact of gaseous nitrogen deposition on plant functioning

Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans

Differential effects of nitrogen forms on cell wall phosphorus remobilization in rice (Oryza sativa) are mediated by nitric oxide, pectin content and the expression of the phosphate transporter OsPT2

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Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus

Abstract: NO is a widespread messenger molecule in physiology. We were interested in investigating whether an NOgenerating system could be present in plants. NO

Cited by 284 publications

References 30 publications

Impact of gaseous nitrogen deposition on plant functioning

Impact of gaseous nitrogen deposition on plant functioning

Differential effects of nitric oxide on peroxidase and H2O2 production by the xylem of Zinnia elegans

Differential effects of nitrogen forms on cell wall phosphorus remobilization in rice (Oryza sativa) are mediated by nitric oxide, pectin content and the expression of the phosphate transporter OsPT2

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Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans