Presence of nicotinic, purinergic and dopaminergic receptors and the TASK-1 K+-channel in the mouse carotid body

Kåhlin, Jessica; Eriksson, Lars; Ebberyd, Anette; Fagerlund, Malin Jonsson

doi:10.1016/j.resp.2010.05.001

Cited by 16 publications

(16 citation statements)

References 38 publications

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“…1 in Ref. 10). Figure 1A confirms this highly specific finding by Leica TCS SP5 recordings of whole mount immunofluorescence with blood vessels stained in red (anti-CD-31) as well as type I cells in green (anti-TH) with the corresponding large cell nuclei in blue (Hoechst) arranged in the typical type I cell cluster formation of the intact mouse carotid body.…”

Section: Identification Of Type I Cellssupporting

confidence: 79%

“…1 in Ref. 10). Other means of identifying type I cells, such as TH-green fluorescent protein (GFP) staining, cannot be used because of the overlapping excitation and emission spectra of GFP and the dyes used (unpublished observation).…”

Section: Discussionmentioning

confidence: 99%

“…1 in Ref. 10) showing mouse CB type I cell cluster with TH staining at the outer part and a dark central corresponding to the cell nucleus.…”

Section: Identification Of Type I Cellsmentioning

confidence: 99%

“…Under hypoxic conditions, various oxygensensitive potassium channels in type I CB cells are inhibited (26). Putative oxygen-sensitive potassium channels have been described for the mouse CB and other species such as Kv3 (19) and the TASK-1/3 K ϩ channels (2,13,16) colocalizing with nicotinergic, purinergic, and dopaminergic receptors in mouse type I cells (10). The closing of potassium channels as the first step for hypoxia-induced transmitter release might be mediated, for example, by oxygen radicals (9), CO (12,29), H 2 S (15), or an AMP kinase (22).…”

mentioning

confidence: 99%

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Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo

Wotzlaw

Bernardini

Berchner‐Pfannschmidt

et al. 2011

American Journal of Physiology-Cell Physiology

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Carotid body (CB) type I cell hypoxia-sensing function is assumed to be based on potassium channel inhibition. Subsequent membrane depolarization initiates an intracellular calcium increase followed by transmitter release for excitation of synapses with linked nerve endings. Several reports, however, contradict this generally accepted concept by showing that type I cell oxygen-sensing properties vary significantly depending on the method of their isolation. We report therefore for the first time noninvasive mapping of the oxygen-sensing properties of type I cells within the intact adult mouse CB ex vivo by using multifocal Nipkow disk-based imaging of oxygen-, calcium- and potential-sensitive cellular dyes. Characteristic type I cell clusters were identified in the compact tissue by immunohistochemistry because of their large cell nuclei combined with positive tyrosine hydroxylase staining. The cellular calcium concentrations in these cell clusters either increased or decreased in response to reduced tissue oxygen concentrations. Under control conditions, cellular potential oscillations were uniform at ∼0.02 Hz. Under hypoxia-induced membrane depolarization, these oscillations ceased. Simultaneous increases and decreases in potential of these cell clusters resulted from spontaneous burstlike activities lasting ∼1.5 s. type I cells, identified during the experiments by cluster formation in combination with large cell nuclei, seem to respond to hypoxia with heterogeneous kinetics.

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Section: Identification Of Type I Cellssupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

“…1 in Ref. 10) showing mouse CB type I cell cluster with TH staining at the outer part and a dark central corresponding to the cell nucleus.…”

Section: Identification Of Type I Cellsmentioning

confidence: 99%

mentioning

confidence: 99%

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Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo

Wotzlaw

Bernardini

Berchner‐Pfannschmidt

et al. 2011

American Journal of Physiology-Cell Physiology

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“…Dopamine binds to D 2 receptors to inhibit L-type Ca 2+ channels in rat isolated glomus cells and to 60 decrease acetylcholine release from rabbit CBs (Benot and López-Barneo, 1990; Kim et al, 2004). Existence of dopamine D 2 receptors in CBs has been confirmed (Lazanov et al, 2009;Kåhlin et al, 2010). Moreover, in vivo experiments demonstrated that dopamine is released from glomus cells during Page 4 of 30 A c c e p t e d M a n u s c r i p t Wakai J. et al,Page 4 hypoxia (Fidone et al, 1982;Iturriaga and Alcayaga, 1998;Buerk et al, 1998;65 Wang and Bisgard, 2002;Powell, 2007), and dopamine content in the CB is increased by exposure to sustained hypoxia for 2-28 days (Hanbauer et al, 1981;Olson et al, 1983;Pequignot et al, 1987;Hui et al, 2003).…”

mentioning

confidence: 92%

Differences in tyrosine hydroxylase expression after short-term hypoxia, hypercapnia or hypercapnic hypoxia in rat carotid body

Wakai

Kizaki

Yamaguchi-Yamada

et al. 2010

Respiratory Physiology & Neurobiology

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Peripheral Chemoreceptors: Function and Plasticity of the Carotid Body

Kumar

Prabhakar

2012

Comprehensive Physiology

453

572

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The discovery of the sensory nature of the carotid body dates back to the beginning of the 20th century. Following these seminal discoveries, research into carotid body mechanisms moved forward progressively through the 20th century, with many descriptions of the ultrastructure of the organ and stimulus-response measurements at the level of the whole organ. The later part of 20th century witnessed the first descriptions of the cellular responses and electrophysiology of isolated and cultured type I and type II cells, and there now exist a number of testable hypotheses of chemotransduction. The goal of this article is to provide a comprehensive review of current concepts on sensory transduction and transmission of the hypoxic stimulus at the carotid body with an emphasis on integrating cellular mechanisms with the whole organ responses and highlighting the gaps or discrepancies in our knowledge. It is increasingly evident that in addition to hypoxia, the carotid body responds to a wide variety of blood-borne stimuli, including reduced glucose and immune-related cytokines and we therefore also consider the evidence for a polymodal function of the carotid body and its implications. It is clear that the sensory function of the carotid body exhibits considerable plasticity in response to the chronic perturbations in environmental O2 that is associated with many physiological and pathological conditions. The mechanisms and consequences of carotid body plasticity in health and disease are discussed in the final sections of this article.

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Presence of nicotinic, purinergic and dopaminergic receptors and the TASK-1 K+-channel in the mouse carotid body

Cited by 16 publications

References 38 publications

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo

Differences in tyrosine hydroxylase expression after short-term hypoxia, hypercapnia or hypercapnic hypoxia in rat carotid body

Peripheral Chemoreceptors: Function and Plasticity of the Carotid Body

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