Presence of membrane and soluble forms of Fas ligand and of matrilysin (MMP-7) activity in normal and abnormal human semen

Riccioli, Anna; Secco, Valentina Dal; Cesaris, Paola De; Starace, Donatella; Gandini, L.; Lenzi, Andrea; Dondero, F.; Padula, Fabrizio; Filippini, Antonio; Ziparo, Elio

doi:10.1093/humrep/dei149

Cited by 14 publications

(21 citation statements)

References 47 publications

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“…MMP7 presents a broad spectrum of actions (reviewed in Ii et al (2006)), including a role in apoptosis, cell proliferation, and release of growth factors. In the male reproductive tract, it has been demonstrated that MMP7 is important for testicular integrity and fertility (Rudolph-Owen et al 1998), and it is present in human semen (Riccioli et al 2005). Our immunohistochemical studies show that MMP7 expression is expressed only in epithelial cells of the vas deferens.…”

Section: Discussionmentioning

confidence: 49%

Locally produced relaxin may affect testis and vas deferens function in rats

Cardoso¹,

Nascimento²,

Royer³

et al. 2010

Reproduction

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We have previously shown that the rat testis and vas deferens contain high levels of the relaxin receptor, RXFP1. The present study was undertaken to determine the expression of relaxin in these tissues, and the effect of exogenous relaxin on Sertoli cell proliferation and on the mRNA levels of some proteins that may contribute to epithelial secretion and tissue reorganization in the vas deferens. Relaxin mRNA levels in testis and vas deferens were much lower than in the prostate. Sertoli cells seem to be an important source of relaxin mRNA in testis. Relaxin immunoreactivity was detected in the seminiferous epithelium but not in the interstitial compartment. The relaxin precursor was expressed in the vas deferens, and relaxin immunoreactivity was detected in apical cells of the vas deferens. Castration, but not treatment with the anti-estrogen ICI 182,780, dramatically reduced relaxin mRNA levels in the prostate and vas deferens, and this effect was prevented by testosterone. Rxfp1 mRNA levels in the vas deferens and prostate were not affected by castration or treatment with ICI 182,780. Exogenous relaxin increased the incorporation of 3 H-thymidine in cultured Sertoli cells, and treatment of the vas deferens with 100 ng/ml relaxin increased the mRNA levels for the cystic fibrosis chloride channel (cystic fibrosis transmembrane regulator) about three times, and doubled mRNA levels for the inducible form of nitric oxide synthase and metalloproteinase 7. These results suggest that locally produced relaxin acts as an autocrine or paracrine agent in the testis and vas deferens to affect spermatogenesis and seminal fluid composition.

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Section: Discussionmentioning

confidence: 49%

Locally produced relaxin may affect testis and vas deferens function in rats

Cardoso¹,

Nascimento²,

Royer³

et al. 2010

Reproduction

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“…In the efferent ductules, treatment with ICI 182,780 dramatically increased the expression of MMP7 in the apical region of the epithelium and in the lumen. MMP7 may have several potential functions in these compartments, including regulation of cell proliferation, which has been shown in MDCK kidney epithelial polarized cells [62], release of growth factors and other proteins, cell apoptosis, and an action on spermatozoa, which appears compatible with the presence of MMP7 activity in human semen [60].…”

Section: Discussionmentioning

confidence: 76%

“…MMP7 presents a broad spectrum of actions [49], and studies with MMP7-null mice have provided support for a physiological role for MMP7 during re-epithelialization, apoptosis, and tumorigenesis [55][56][57]. In the male reproductive tract, MMP7 is expressed in a variety of tissues and is present in human semen [58][59][60]. The importance of MMP7 for testicular integrity has been demonstrated by studies with transgenic animals overexpressing MMP7 [59], which presented disintegration of the testicular interstitial tissue and infertility.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Antiestrogen Fulvestrant (ICI 182,780) on Gene Expression of the Rat Efferent Ductules1

Yasuhara

Gomes

Siu

et al. 2008

Biology of Reproduction

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The efferent ductules express the highest amount of estrogen receptors ESR1 (ERalpha) and ESR2 (ERbeta) within the male reproductive tract. Treatment of rats with the antiestrogen fulvestrant (ICI 182,780) causes inhibition of fluid reabsorption in the efferent ductules, leading to seminiferous tubule atrophy and infertility. To provide a more comprehensive knowledge about the molecular targets for estrogen in the rat efferent ductules, we investigated the effects of ICI 182,780 treatment on gene expression using a microarray approach. Treatment with ICI 182,780 increased or reduced at least 2-fold the expression of 263 and 98 genes, respectively. Not surprisingly, several genes that encode ion channels and macromolecule transporters were affected. Interestingly, treatment with ICI 182,780 markedly altered the expression of genes related to extracellular matrix organization. Matrix metalloproteinase 7 (Mmp7), osteopontin (Spp1), and neuronal pentraxin 1 (Nptx1) were among the most altered genes in this category. Upregulation of Mmp7 and Spp1 and downregulation of Nptx1 were validated by Northern blot. Increase in Mmp7 expression was further confirmed by immunohistochemistry and probably accounted for the decrease in collagen content observed in the efferent ductules of ICI 182,780-treated animals. Downregulation of Nptx1 probably contributed to the extracellular matrix changes and decreased amyloid deposition in the efferent ductules of ICI 182,780-treated animals. Identification of new molecular targets for estrogen action may help elucidate the regulatory role of this hormone in the male reproductive tract.

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“…Among the 7 species observed in the present study, the presence of Fas receptors in mature spermatozoa has been reported in human (Sakkas et al 1999;McVicar et al 2004;Starace et al 2009;Soleimani et al 2010), bovine ), sheep (Zan Bar et al 2008, and mice (Starace et al 2009), but it has not been observed in goat and rat, to our knowledge. Similarly, the presence of FasL in mature spermatozoa has been reported in human (Riccioli et al 2005), mouse (D'Alessio et al 2001;Riccioli et al 2003), and cattle ), but it has not been observed in pig, sheep, goat, and rat.…”

Section: Discussionmentioning

confidence: 68%

Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

Li¹,

Sun²,

Zhu³

et al. 2017

CZECH J ANIM SCI

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Li Y., Sun M., Zhu J., Jiao G., Lin J., Dong H., Tan J., Zhou J. Although it has been proposed that the Fas and Fas ligand (FasL) may protect ejaculated spermatozoa against apoptosis induced by lipoperoxidative damage and against lymphocytes present in the female genital tract, studies reported conflicting results on the presence of Fas receptors in ejaculated human spermatozoa. Furthermore, the expression of Fas/FasL on mature spermatozoa has not been observed in several important mammals. Using seven species, we observed the possibility for species difference in Fas/FasL expression on mature spermatozoa by both immunofluorescence microscopy and western blot analysis. Whereas intensive signals of Fas immunolabelling were detected in sperm head and middle piece and weak signals observed in the tail in 86-100% of the mouse, rat, bull, ram, and buck spermatozoa, only weak signals were detected on the whole body of 27% boar spermatozoa and in the head of 21% human spermatozoa. The pattern of FasL localization was identical to that of Fas in spermatozoa from human, mouse, rat, ram, and buck, but boar and bull spermatozoa showed weak and intensive FasL signals, respectively, only in the head. Western blotting further confirmed the Fas and FasL expression in mouse, rat, bull, ram, and buck, but not in human and boar spermatozoa. Taken together, the results revealed a marked species difference in Fas/FasL expression and an extensive co-expression of Fas and FasL among mature mammalian spermatozoa, suggesting that whereas spermatozoa from most species may be protected by Fas/FasL, those from human and boar may not use the Fas system for protection.

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Presence of membrane and soluble forms of Fas ligand and of matrilysin (MMP-7) activity in normal and abnormal human semen

Cited by 14 publications

References 47 publications

Locally produced relaxin may affect testis and vas deferens function in rats

Locally produced relaxin may affect testis and vas deferens function in rats

Effects of the Antiestrogen Fulvestrant (ICI 182,780) on Gene Expression of the Rat Efferent Ductules1

Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa

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