Presence of mature sperm in testicular parenchyma of men with nonobstructive azoospermia: Prevalence and predictive factors

Mulhall, John P.; Burgess, C.M.; Cunningham, D.L.; Carson, Ronald S.; Harris, Doria; Oates, Robert D.

doi:10.1016/s0090-4295(96)00356-1

Cited by 114 publications

(67 citation statements)

References 19 publications

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“…Because of this, strategies have been developed to more accurately determine which men with failing testes are candidates for IVF-ICSI, and surgical techniques have been refined to minimize the invasiveness of sperm harvest procedures. One strategy involves taking as many biopsy samples as needed either before IVF-ICSI (and freezing the sperm for later thaw and use), or at the time of ICSI (28). Microdissection TESE uses microsurgical exploration of the widely opened testis to search for pockets of sperm (29).…”

Section: Genetic Male Infertilitymentioning

confidence: 99%

Definition and current evaluation of subfertile men

Shefi

Turek

2006

Int. braz j urol.

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Male infertility affects 10% of reproductive aged couples worldwide and is treatable in many cases. In addition to other well-described etiologies, genetic causes of male infertility are now more commonly diagnosed. Using principles of evidencebased medicine, this review outlines diagnostic and treatments options to inform clinical management. In order of importance, randomized controlled clinical trials, basic scientific studies, meta-analyses, case-controlled cohort studies, best-practice policy recommendations and reviews from peer-reviewed literature were incorporated that provide organized and timely guidelines to the current management of male infertility. The strength of evidence for treatment recommendations is also classified when appropriate.

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Section: Genetic Male Infertilitymentioning

confidence: 99%

Definition and current evaluation of subfertile men

Shefi

Turek

2006

Int. braz j urol.

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“…Fine needle aspiration (FNA) has been employed for more precise systemic testicle sampling to gain local information about spermatogenesis and to assess for sperm suitable in ART (31). In general, when merely spermatids were seen in pure patterns of testicle histology, spermatozoa could be detected in FNA (32). Constant improvements to focally localize presumptive testicular areas for sperm retrieval have been reported during the past years.…”

Section: Discussionmentioning

confidence: 99%

Tissue Perfusion Essential for Spermatogenesis and Outcome of Testicular Sperm Extraction (TESE) for Assisted Reproduction

Herwig

Tosun

Pinggera

et al. 2004

J Assist Reprod Genet

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Purpose : In order to determine if there are areas of major and minor perfusion in a single testicle and if the quality of sperm is correlated with quantity of perfusion we collected testicle tissue for TESE in accordance to the local testicle tissue perfusion. Methods : A patient undergoing TESE underwent testicular perfusion mapping using contrast enhanced ultrasound. The exposed tissue was scanned with a Laser Doppler scanner and perfusion rates were determined measuring tissue perfusion units (TPUs). Tissue was biopsied and sperm were selected and prepared for assisted reproduction. Results : The total amount of isolated sperm correlated highly with the intensity of tissue perfusion showing high number of sperm in areas with high TPUs. Conclusions : This is the first demonstration that sperm quality and quantity is depending on tissue perfusion within the testicle. To further improve infertility treatment we propose that random biopsies could be replaced by perfusion-dependent collection of testicular tissue.

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“…Esto provee evidencia de que una deleción en Yq puede originar una expresión fenotípica variable en los diferentes individuos analizados dentro de un mismo núcleo familiar (4,30,41), lo que es clínicamente significativo, ya que la presencia de una deleción no es absolutamente un marcador para infertilidad y puede estar asociado con aparente fertilidad que en la segunda o tercera etapa de la vida y puede conducir al desarrollo de un fenotipo testicular severo como azoospermia no obstructiva (42,43). Las deleciones intersticiales pueden ocurrir durante la meiosis por un empaquetamiento defectuoso del DNA en la espermiogénesis.…”

Section: Discussionunclassified

Determinación de deleciones en el cromosoma Y en hombres infértiles candidatos a técnicas de reproducción asistida

Pérez

First

2004

nova

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IntroducciónLos estudios genéticos actuales se realizan para la detección y el análisis de la información genética a nivel molecular, estos permiten detectar las causas de enfermedades o desórdenes que padecen las personas.La búsqueda de tal información la lideran grandes grupos de investigadores, Proyecto Genoma Humano entre otros, los cuales han identificado y secuenciado los genes presentes en los cromosomas, entre ellos el cromosoma Y motivo del presente estudio (1). ResumenEl compromiso espermático severo en el hombre infértil, puede ser el resultado de la presencia de deleciones en el cromosoma Y en la región AZFc (Factor de azoospermia región c). En este estudio se caracterizaron 97 hombres con infertilidad con una frecuencia del 6% para la presencia de deleciones en la población estudiada. De estos hombres positivos para deleción en la región Yq (AZFc), nacieron tres niños mediante el uso de inyección intracitoplasmática (ICSI) que heredaron la mutación en el cromosoma Y, fundamentando la importancia de crear un programa de tamizaje para detectar la presencia de deleción en este cromosoma en la población de hombres infértiles candidatos para técnica de fertilización asistida (ART) mediante ICSI. Palabras claves: hombres infértiles, delección, Zona Yq, fertilización asistida, inyección intracitoplasmática de espermatozoides.Abstract Determination of microdeletion in chromosome Y in infertile men, candidates to assisted reproduction techniques Intracytoplasmatic spermatozoid Injection.Severe spermatic damage in infertile man can be the result of presence of deletion in chromosome Y and in AFZc region (Zoosperm factor Zone C). This study was made in 97 men with infertility and a frequency of 6% to the presence of deletion in the studied population. Of those men positive to deletion in Zone Yq (AZFc)were born three children by using intracytoplasmatic injection (ICSI), whom inherited mutation in chromosome Y, it established the importance to create a sifting program to detect the presence of deletion in this chromosome infertile men population, candidates to Assisted Reproduction Technique treatments (ART) by ICSI.

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Presence of mature sperm in testicular parenchyma of men with nonobstructive azoospermia: Prevalence and predictive factors

Cited by 114 publications

References 19 publications

Definition and current evaluation of subfertile men

Definition and current evaluation of subfertile men

Tissue Perfusion Essential for Spermatogenesis and Outcome of Testicular Sperm Extraction (TESE) for Assisted Reproduction

Determinación de deleciones en el cromosoma Y en hombres infértiles candidatos a técnicas de reproducción asistida

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