Presence of leptin receptors in rat small intestine and leptin effect on sugar absorption

Lostao, M. P.; Urdaneta, Elena; Martı́nez-Ansó, Eduardo; Barber, A; Martínez, Joseline

doi:10.1016/s0014-5793(98)00110-0

Cited by 110 publications

(97 citation statements)

References 31 publications

(42 reference statements)

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“…Lostao et al (1998) demonstrated that leptin has a rapid inhibitory effect on -galactose uptake in the rat intestinal mucosa in vitro. In the same year, Morton et al (1998) showed that leptin can reduce the transcription of genes responsible for apolipoprotein biosynthesis (APO-AIV) in the enterocytes in mouse jejunum in response to olive oil administration.…”

Section: Figurementioning

confidence: 99%

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Exogenous leptin controls the development of the small intestine in neonatal piglets

Woliński¹,

Biernat²,

Guilloteau³

et al. 2003

Journal of Endocrinology

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Leptin, a hormone produced and secreted by adipose tissue, muscles and stomach, is involved in the regulation of adipose tissue mass, food intake and body weight in neonatal animals. It is also produced in the mammary glands and secreted into the colostrum and milk. Since leptin receptors are widely distributed in the small intestine mucosa, the aim of the present study was to investigate the effect of exogenous leptin on the development of the small intestine in neonatal piglets. Male neonatal piglets were fed with sow's milk or artificial milk formula. Every 8 h the latter received either vehicle or leptin (2 or 10 µg/kg body weight). The animals were either killed after 6 days of treatment and the small intestine sampled for histology and brush border enzyme activities or were tested for marker molecule (Na-fluorescein and BSA) absorption in vivo. Feeding milk formula slowed the maturation of small intestinal mucosa compared with feeding sow's milk. However, after leptin treatment the length of the small intestine was increased, and intestinal villi length, but not crypt size, was reduced compared with controls. The mitotic index was increased and the percentage of vacuolated enterocytes was reduced in the entire small intestine. Enterocyte brush border protease and lactase activities were reduced in the jejunum. Nafluorescein marker molecule absorption did not change but that of BSA was reduced 3·8-fold. In conclusion, exogenous leptin administered in physiological doses reversed the maturation of the small intestinal mucosa to the range found in sow-reared piglets.

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Section: Figurementioning

confidence: 99%

“…accumulation indirectly through a local mechanism in the small intestine controlling the absorption of nutrients (Lostao et al 1998, Morton et al 1998 or intestinal motility (Woliński et al 2001). Lostao et al (1998) demonstrated that leptin has a rapid inhibitory effect on -galactose uptake in the rat intestinal mucosa in vitro.…”

Section: Figurementioning

confidence: 99%

Exogenous leptin controls the development of the small intestine in neonatal piglets

Woliński¹,

Biernat²,

Guilloteau³

et al. 2003

Journal of Endocrinology

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“…The functional OB-R is predominantly expressed in the jejunum and more weakly in the ileum, the two major sites that are involved in nutrient handling. In this context, it has been shown that leptin produced a rapid inhibitory effect on sugar absorption in rat intestinal rings [68]. Likewise, leptin causes a rapid activation of STAT5 in jejunum, which is associated with a reduction of the apolipoprotein-AIV transcript expression 90 min after ingestion of a pure fat load [67].…”

Section: Leptin Action On Gutmentioning

confidence: 99%

Relation between leptin and the regulation of glucose metabolism

2000

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OverviewThe identification of the ob gene through positional cloning [1] and the discovery that its encoded protein, leptin, is essential for body weight homeostasis [2±4] have permanently altered the field of metabolic physiology. Over a 5-year period a substantial and rapidly changing body of knowledge has been created.Leptin, a 16 kDa circulating hormone produced and released primarily by adipocytes, exerts a regulatory control on food intake and energy expenditure [2±4]. Plasma leptin concentrations are correlated with total fat mass, per cent body fat and body mass index acting as a sensing hormone or ªlipostatº in a negative feedback control from adipose tissue to the hypothalamus, the brain centre responsible for satiety [5,6]. Thus, leptin informs the brain about the abundance of body fat, thereby allowing feeding behaviour, metabolism and endocrine physiology to be coupled to the nutritional state of the organism. Leptin-deficient ob/ob mice exogenously treated with leptin have a pronounced body weight loss with a distinct loss of discernible body fat [2±4]. This effect is not only attributable to a decreased food intake but also to an increased basal metabolic rate with selective promotion of fat metabolism [2, 7±10].Leptin was discovered through a very specific biological action consisting in its involvement in body weight and appetite regulation. Interestingly, leptin has structural similarities to the family of helical cytokines [11]. Many cytokines, originally isolated through particular biological actions, have subsequently been shown to be capable of stimulating a variety of biological responses in a wide spectrum of cell types. Thus, leptin shares with other cytokines an extreme functional pleiotropy and has been shown to be involved in quite diverse physiological functions, such as reproduction [12], angiogenesis [13], haematopoiesis [14] and immune responsiveness [15].Consistent with leptin's role in controlling appetite and energy metabolism, leptin receptors (OB-R) have been found in the hypothalamus and adjacent brain regions [16,17]. At the beginning direct leptin actions were thought to be exclusively confined to the central nervous system (CNS). The almost ubiquitous distribution of functional OB-R provides, however, evidence for a multiplicity of peripheral target organs. At the cellular level OB-R, structurally related to the family of cytokine receptors, have been found to activate Janus kinases and to function as a signal transducer and activator of transcription (STAT) pathways [16]. Special attention among the extraneural tissues expressing functional OB-R should be given to organs involved in metabolism and digestion like the pancreas, skeletal muscle and the gastrointestinal system [16,17]. The pancreas has evolved a complex and exquisitely sensitive mechanism for matching the stimulation or inhibition of pancreatic hormone release to the prevailing metabolic needs [18]. Pancreatic endocrine and exocrine secretion is released in response to nutrient inflow from the gut and to g...

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“…In the juvenile rat, leptin receptor expression has been found in several tissues including small intestine (Lostao et al, 1998). In mice, expression of the functional isoform leptin receptor is restricted to the jejunum and is readily detected in isolated enterocytes from this site by the RT-PCR technique (Morton et al, 1998), suggesting that the epithelium of the jejunum is a direct target of leptin action.…”

Section: Leptin Function In the Juvenile Gutmentioning

confidence: 99%

“…In mice, expression of the functional isoform leptin receptor is restricted to the jejunum and is readily detected in isolated enterocytes from this site by the RT-PCR technique (Morton et al, 1998), suggesting that the epithelium of the jejunum is a direct target of leptin action. Indeed, Lostao et al (1998) have originally demonstrated that leptin has a rapid inhibitory effect on sugar absorption in the rat intestinal mucosa in vitro. In their study, leptin inhibited D-galactose uptake by rat small intestinal rings, respectively by 33 and 56% after 5 and 30 min of incubation!…”

Section: Leptin Function In the Juvenile Gutmentioning

confidence: 99%

Bioactive peptides in young animal nutrition

Zabielski¹

2001

J. Anim. Feed Sci.

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Maternal milk contains a number of bioactive peptides and proteins important for the development of the gastrointestinal tract. This article briefly reviews information on milk insulin, insulinlike growth factors, and epidermal growth factor. Since recently leptin has been found in maternal milk, a more detailed overview of leptin's effects on gastrointestinal tract function is presented. The physiological importance of milk leptin needs, however, further investigation. In contrast to milk, artificial milk formulas contain markedly lower amounts of bioactive peptides.

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Presence of leptin receptors in rat small intestine and leptin effect on sugar absorption

Cited by 110 publications

References 31 publications

Exogenous leptin controls the development of the small intestine in neonatal piglets

Exogenous leptin controls the development of the small intestine in neonatal piglets

Relation between leptin and the regulation of glucose metabolism

Bioactive peptides in young animal nutrition

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