Presence of Laminin Receptors in Staphylococcus aureus

Abstract

A characteristic feature of infection by Staphylococcus aureus is bloodstream invasion and widespread metastatic abscess formation. The ability to extravasate, which entails crossing the vascular basement membrane, appears to be critical for the organism's pathogenicity. Extravasation by normal and neoplastic mammalian cells has been correlated with the presence of specific cell surface receptors for the basement membrane glycoprotein laminin. Similar laminin receptors were found in Staphylococcus aureus but not in Staphylococcus epidermidis, a noninvasive pathogen. There were about 100 binding sites per cell, with an apparent binding affinity of 2.9 nanomolar. The molecular weight of the receptor was 50,000 and pI was 4.2. Eukaryotic laminin receptors were visualized by means of the binding of S. aureus in the presence of laminin. Prokaryotic and eukaryotic invasive cells might utilize similar, if not identical, mechanisms for invasion.

Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus

“…These properties imply that S. aureus is capable of interacting with host tissue components. Consistent with this notion is the finding that S. aureus binds specifically to major ECM components such as collagen (16), fibronectin (17), laminin (18), proteoglycans (19), and elastin (20). Importantly, there is increasing evidence supporting a relationship between the ability of S. aureus to bind to ECM and its pathogenicity.…”

Isolation of Potential ProbioticLactobacillusrhamnosusStrains from Traditional Fermented Mare Milk Produced in Sumbawa Island of Indonesia

“…14) They are frequently targeted by pathogenic bacteria that express surface proteins with affinity for ECM proteins. [15][16][17] In this context, the ability to adhere ECM proteins expressed on the surface of BM is one of the most important criteria in selecting probiotics, which potentially interfere with infection pathogenic bacteria in the GI tract. 18) Fermented dairy products are believed to be promising sources of probiotics because of their history in the human diet and functionality.…”

Isolation and Characterization of Teichoic Acid‐Like Substance as an Adhesin of Staphylococcus aureus to HeLa Cells

“…Staphylococcus aureus has three known adhesins, the laminin receptor (21), fibronectin receptor (13,14,22,24,25), and collagen receptor (17,26), and some putative adhesins, including teichoic acid (2,3,(6)(7)(8), lipoteichoic acid (9), protein A (11), etc. S. aureus may have other adhesins because host cells have many kinds of molecules other than the known extracellular matrices on the surface.…”