Presence of K‐virus in Wild Mice in Australia

Holt, Dolores.

doi:10.1038/icb.1959.20

Cited by 25 publications

(12 citation statements)

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“…Viral replication during acute K virus infection occurs almost exclusively in vascular endothelial cells, and renal involvement is confined to endothelia of glomerular and parenchymal capillaries (Fisher & Kilham, 1953;Kilham & Murphy, 1953;Holt, 1959;Greenlee, 1979Greenlee, , 1981Mokhtarian & Shah, 1980). The finding that nude mice inoculated with K virus develop extensive involvement of renal tubular epithelial cells is thus of interest, since it demonstrates that the cellular tropism of papovaviruses may change during chronic infection.…”

Section: Discussionmentioning

confidence: 99%

“…K virus infection of suckling mice is characterized by extensive infection of pulmonary and systemic vascular endothelial cells. Death results from interstitial pneumonia (Fisher & Kilham, 1953;Kilham & Murphy, 1953;Greenlee, 1979;Holt, 1959;Mokhtarian & Shah, 1980). In older suckling mice, K virus produces a protracted infection in which virus and/or viral antigen can be detected in several organs for as long as 4 months (Greenlee, 1981).…”

Section: Introductionmentioning

confidence: 99%

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Chronic Infection of Nude Mice by Murine K Papovavirus

Greenlee

1986

Journal of General Virology

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SUMMARYNude (nu/nu) mice were inoculated intracranially with l06's newborn mouse 50~ lethal doses of K virus and were studied over a period of 28 weeks using serological methods, virus assay and immunohistological staining for viral antigens. K virus infection of nude mice, although clinically asymptomatic, was slowly progressive despite prompt IgM and IgG antibody response. The highest titres of K virus infectivity were reached in spleens, kidneys and intestines. Vascular endothelial cells represented the major site of viral replication, as has been shown to be the case in immunologically normal mice, with extensive involvement of intestinal capillaries. In addition, however, unlike immunologically normal mice, nude mice inoculated with K virus developed multi focal infection of renal tubular epithelial cells. Nude mice did not develop histologically detectable evidence of central nervous system involvement by K virus, and K virus infection did not result in neoplasia. Infected vascular endothelial cells and renal tubular epithelial cells in animals studied at 16 and 27 weeks after inoculation were grouped in scattered clusters, suggesting local spread of infection. The present study indicates that nude mice with preserved B cell function but impaired T cell-mediated immunity are able to limit systemic dissemination of K virus but are unable to prevent local progression of infection by cell-to-cell spread. K virus is capable of altering its cellular tropism during chronic infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chronic Infection of Nude Mice by Murine K Papovavirus

Greenlee

1986

Journal of General Virology

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“…Three biweekly injections of culture fluids from untransfected q-2 cells, which contain high levels of RNA-free virions, were given to 10 (Table 3).…”

Section: Methodsmentioning

confidence: 99%

“…They were used as dilutions of clarified supernatants from 10% (wt/vol) homogenates of the greatly enlarged spleens of mice infected 12-15 days earlier as described (15). Stocks were titrated by the spleen focus method (16) competent FMuLV proviral genome in clone 2-lalc (10) to produce pKR4 (see Fig. 1 Cytolytic T-lymphocyte (CTL) responses were determined using 51Cr-labeled Df45 cells as targets as described (18).…”

Section: Methodsmentioning

confidence: 99%

Approach to a retrovirus vaccine: immunization of mice against Friend virus disease with a replication-defective Friend murine leukemia virus.

Ruan

Lilly²

1992

Proc. Natl. Acad. Sci. U.S.A.

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In an initial attempt to test the ability of replication-defective retroviruses to immunize against immunologically related pathogenic viruses, we have worked with the erythroleukemogenic Friend retrovirus complex (FV), which consists of a replication-competent helper component, Friend murine leukemia virus (FMuLV), and a related defective pathogenic component, spleen focus-forming virus (SFFV). An 81-base-pair deletion was introduced into the plSE-encoding region of the env gene of an otherwise replication-competent molecular clone of the FMuLV provirus. After transfection of this clone into cells that package the viral RNA in MuLV coats, infectious virus was released into the culture medium. Mouse fibroblasts infected with this virus, here called AFMuLV, expressed the truncated viral env gene products in their cytoplasm but not on cell surfaces, and culture fluids from these cells did not transmit the infection to fresh mouse fibroblasts. In preliminary experiments, immunization of mice of H-2-congenic BALB/c strains with AFMuLV conferred levels of inmnunity to FV dise ranging from weak to relatively strong.Immunized mice developed anti-FV IgM and IgG antibodies and cytotoxic T cells. Mice observed for 15 weeks after the first of two immunizations showed no detectable pathology, but AFMuLV DNA was detectable in livers of some immunized mice for at least 3-6 weeks. These results suggest that our approach to development ofretrovirus vaccines may be a useful one.

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“…Serially passaged Epo virus (obtained by homogenizing infected spleens in 10 vol of 0.02 M Tris HCl, pH 7.4/0.10 M NaCl/0.001 M EDTA and centrifuging twice at 10,000 x g for 15 min) was stored at -70'C. Passaged Friend virus (Lilly-Steeves polycythemia strain) was also from spleen homogenates (14). Mouse strains included NFS/N, DBA/2J (both Fv-2s), and the Fv-2r congenic strain D2.B6-Fv-2r (D2.R).…”

mentioning

confidence: 99%