Presence of Hypoderma lineatum stage I larvae in the esophagus of cattle slaughtered in Chihuahua, Chih., Mexico

Quintero-Martínez, M. T.; Negrete, Jimena Otero; Benı́tez, Rocı́o; Méndez, Miguel Ángel Aguilar; Vega, Gonzalez; Cruz-Vázquez, Carlos

doi:10.1016/j.vetpar.2007.01.012

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…larvae causes a myiasis that is widely spread in the Northern Hemisphere. Despite considerable efforts that have achieved its eradication in eight European countries (1), cattle hypodermosis is still very common in the rest of Europe (2–4), Northern Africa (5), North America (6) and Asia (7), with considerable economic losses for the livestock industry (8) and increased susceptibility to other diseases (1).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production

Fontán

Dacal

López

et al. 2009

Parasite Immunology

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This study examines the immunomodulatory effect of a crude larval extract (CLE), obtained from first stage larvae (L1) of H. lineatum, and the purified fractions hypodermin A (HyA), HyB and HyC. Proliferative responses of peripheral blood mononuclear cells (PBMC) from uninfested and previously infested cattle and the production of the cytokines IL-10, IL-4 and IFN-gamma, in response to concanavalin A (Con A), were determined. The stimulation index of peripheral blood mononuclear cells from uninfested cattle was significantly lower than that from infested animals with the different antigens assayed. The HyA was the antigen that most inhibited the proliferative response, followed by the HyB, the HyC and the CLE. This hypodermin provoked an increase of IFN-gamma and a suppression of IL-10 production that would support a Th1-like cytokine response. The HyB reduced the production of IL-10 stimulated by the Con A in cultures from infested animals. The HyC did not modulate the production of cytokines. Finally, the CLE induced a marked suppression in the production of the different cytokines in cultures from naïve and previously sensitized animals. Our results indicate that Hypoderma larval secretions are comprised of different components (hypodermins) that individually induce distinct but partially overlapping modulatory responses.

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Section: Introductionmentioning

confidence: 99%

Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production

Fontán

Dacal

López

et al. 2009

Parasite Immunology

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“…Similarly, it was 47.8%, 38.9%, 33%, 41.6% and 41.9% in Southeast Anatolia, Central Anatolia, Mediterranean, Agean and East Anatolia [23] . In Spain the seroprevalence was 26-42% [20] . In Turkey, the seroprevalence in Thrace region was 3.56% [24] and 4% in Elazig province [23] , while 43.3% in Vicenza province of North eastern Italy [25] .…”

Section: Discussionmentioning

confidence: 99%

“…Warble fly infestation ranged from 25 to 90% in goats and 21 to 26% in cattle [15] . The infestation rate of hypodermosis varies within Pakistan [15][16][17][18] and many parts of globe [19][20][21] it might be due to the differences in climatic conditions. In Pakistan all the studies in the past were based on traditional method of grub monitoring that has very low sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

Development of Indirect ELISA for the Diagnosis of Bovine Hypodermosis (Hypoderma lineatum) in the Cattle of Subtropical Region of Pakistan

Ahmed

Fontán

López-Sández

et al. 2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L 1 ) of the warble fly were collected from the surrounding abattoirs to obtain the antigen (HyC) used for development of indirect ELISA. The seroprevalence was 17.4% (174/1000). The highest seroprevalence was recorded in the Rawalpindi district (28.81%), followed by Attock (21.51%), Jhelum (10%) and Chakwal (6.66%). In present study the sensitivity and specificity was 97.28% and 96.44%, respectively. The epidemiological factors showed that the village having hilly locations, local breed, young animals, water bodies, extensive grazing, primary exposed and non medicated were more seropositive as compare to others (P<0.05). In conclusion, indirect ELISA developed during this study is very useful tool for early detection of bovine hypodermosis in cattle grazing in subtropical region of Pakistan. Keywords ÖzetBu çalışmanın amacı Hypoderma lineatum'a karşı üretilen antikorları tespit etmek amacıyla bir indirek ELİSA yöntemi geliştirmek ve Pakistan'ın subtropikal bölgelerinde sığır hypodermozisinin seroprevalansı üzerine seroepidemiyolojik faktörlerin etkisini belirlemektir. Bu amaçla Rawalpindi, Attock, Chakwal ve Jhelum bölgelerinde yirmi sekiz köyden toplam 1000 adet kan örneği alındı. Nokra sineğinin ilk instar larvaları (L 1 ) indirek ELİSA yöntemini geliştirmek maksadıyla kullanılacak antijeni (HyC) elde etmek amacıyla çevre kesimhanelerden toplandı. Seroprevalans %17/4 (174/1000) olarak belirlendi. En yüksek prevalans Rawalpindi (%28.81) bölgesinde tespit edilirken bunu Attock (21.51), Jhelum (%10) ve Chakwal (%6.66) takip etti. Bu çalışmada duyarlılık ve özgüllük sırasıyla %97.28 ve %96.44 olarak tespit edildi. Epidemiyoojik faktörlerden tepelik yerleşimli köyler, lokal ırklar, genç hayvanlar, su kaynakları, geniş meralar, primer maruz kalanlar ile ilaçlanmamış hayvanlarda diğerlerine göre seroprevalanslık daha yüksek olarak belirlendi (P<0.05). Sonuç olarak, bu çalışmada geliştirilen indirek ELİSA Pakistan'ın subtropikal bölgelerinde sığır hypodermozisinin erken tespitinde oldukça yararlıdır.

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An overview on different aspects of hypodermosis: Current status and future prospects

et al. 2016

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Presence of Hypoderma lineatum stage I larvae in the esophagus of cattle slaughtered in Chihuahua, Chih., Mexico

Cited by 4 publications

References 6 publications

Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production

Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production

Development of Indirect ELISA for the Diagnosis of Bovine Hypodermosis (Hypoderma lineatum) in the Cattle of Subtropical Region of Pakistan

An overview on different aspects of hypodermosis: Current status and future prospects

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