Presence of High Numbers of Transcriptionally Active <i>Helicobacter pylori</i> in Vomitus from Bangladeshi Patients Suffering from Acute Gastroenteritis

Janzon, Anders; Bhuiyan, Taufiq; Lundgren, Anna; Qadri, Firdausi; Svennerholm, Ann‐Mari; Sjöling, Åsa

doi:10.1111/j.1523-5378.2009.00692.x

Cited by 19 publications

(15 citation statements)

References 48 publications

(63 reference statements)

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“…Increased rates of H. pylori infection in developing nations such as Pakistan, Vietnam, Bangladesh and India, which combat poverty, poor sanitary conditions and overcrowding, indicate the role of environmental factors in the transmission of infection [17][18][19][20]. In 2007, Ahmed et al indicated that the risk of H. pylori infection can be reduced by improving personal hygiene and regular use of boiled drinking water [20].…”

Section: Resultsmentioning

confidence: 99%

Presence of Helicobacter pylori in drinking water of Karachi, Pakistan

Khan

Farooqui

Kazmi

2011

J Infect Dev Ctries

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Background: Prevalence of Helicobacter pylori (H. pylori) infection is an increasing problem in developing countries. Several environmental factors such as overcrowding, poverty, contaminated drinking water and food are considered to contribute toward transmission of infection; however, little is known about their definitive roles. The aim of this study is to investigate the presence of H. pylori in drinking water samples of Karachi, Pakistan. Methodology: Samples of drinking water were collected from 18 different towns located in the metropolitan area of Karachi. Samples were concentrated by membrane filtration method and subjected to PCR for the detection of H. pylori. Results: Two out of 50 (4%) samples collected from two different densely populated town areas were found to be positive for H. pylori. Conclusion: The study provides evidence for the presence of H. pylori in municipal drinking water of Karachi.

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Section: Resultsmentioning

confidence: 99%

Presence of Helicobacter pylori in drinking water of Karachi, Pakistan

Khan

Farooqui

Kazmi

2011

J Infect Dev Ctries

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“…For relative expression of imaA and ureA, transcript levels were normalized to the levels of groEL (69) in each sample. Transcripts were amplified with HP0289 For1.1 (For stands for forward) (5=-TAACGATCCAAAACGCT TCC) and HP0289 Rev1.1 (Rev stands for reverse) (5=-TCCCTTGAGGC GAGAGTGATT), UreA F1 and UreA R1 (36), and groEL F (JVO-529) and groEL R (JVO-5298) (69). Il8 (55) and TNF-␣ (84) expression levels from AGS cells were normalized to 18S (55) rRNA, and KC levels from mouse tissues were normalized to GAPDH (24).…”

Section: Methodsmentioning

confidence: 99%

The Helicobacter pylori Autotransporter ImaA (HP0289) Modulates the Immune Response and Contributes to Host Colonization

2012

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ABSTRACTThe human pathogenHelicobacter pyloriemploys a diverse collection of outer membrane proteins to colonize, persist, and drive disease within the acidic gastric environment. In this study, we sought to elucidate the function of the host-induced geneHP0289, which encodes an uncharacterized outer membrane protein. We first generated an isogenicH. pylorimutant that lacksHP0289and found that the mutant has a colonization defect in single-strain infections and is greatly outcompeted in mouse coinfection experiments with wild-typeH. pylori. Furthermore, we used protease assays and biochemical fractionation coupled with an HP0289-targeted peptide antibody to verify that the HP0289 protein resides in the outer membrane. Our previous findings showed that theHP0289promoter is upregulated in the mouse stomach, and here we demonstrate thatHP0289expression is induced under acidic conditions in an ArsRS-dependent manner. Finally, we have shown that theHP0289mutant induces greater expression of the chemokine interleukin-8 (IL-8) and the cytokine tumor necrosis factor alpha (TNF-α) in gastric carcinoma cells (AGS). Similarly, transcription of the IL-8 homolog keratinocyte-derived chemokine (KC) is elevated in murine infections with the HP0289 mutant than in murine infections with wild-typeH. pylori. On the basis of this phenotype, we renamed HP0289 ImaA forimmunomodulatoryautotransporter protein. Our work has revealed that genes inducedin vivoplay an important role inH. pyloripathogenesis. Specifically, the outer membrane protein ImaA modulates a component of the host inflammatory response, and thus may allowH. pylorito fine tune the host immune response based on ImaA expression.

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“…Helicobacter pylori strains P12 and the Nicaraguan clinical isolate Nic25_A were cultured on Columbia blood agar plates under microaerophilic conditions (80% N 2 , 10% CO 2 , 10% H 2 ), which is standard for H. pylori (Janzon et al, 2009). The strains were replated and cultured for 24 h before the colony biomass was scraped from the plates and suspended in 500 μL of phosphate buffered saline.…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Two Helicobacter pylori Strains using Genomics and Mass Spectrometry-Based Proteomics

et al. 2016

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Helicobacter pylori, a gastroenteric pathogen believed to have co-evolved with humans over 100,000 years, shows significant genetic variability. This motivates the study of different H. pylori strains and the diseases they cause in order to identify determinants for disease evolution. In this study, we used proteomics tools to compare two H. pylori strains. Nic25_A was isolated in Nicaragua from a patient with intestinal metaplasia, and P12 was isolated in Europe from a patient with duodenal ulcers. Differences in the abundance of surface proteins between the two strains were determined with two mass spectrometry-based methods, label-free quantification (MaxQuant) or the use of tandem mass tags (TMT). Each approach used a lipid-based protein immobilization (LPITM) technique to enrich peptides of surface proteins. Using the MaxQuant software, we found 52 proteins that differed significantly in abundance between the two strains (up- or downregulated by a factor of 1.5); with TMT, we found 18 proteins that differed in abundance between the strains. Strain P12 had a higher abundance of proteins encoded by the cag pathogenicity island, while levels of the acid response regulator ArsR and its regulatory targets (KatA, AmiE, and proteins involved in urease production) were higher in strain Nic25_A. Our results show that differences in protein abundance between H. pylori strains can be detected with proteomic approaches; this could have important implications for the study of disease progression.

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Presence of High Numbers of Transcriptionally Active Helicobacter pylori in Vomitus from Bangladeshi Patients Suffering from Acute Gastroenteritis

Abstract: We conclude that high numbers of transcriptionally active H. pylori are shed in vomitus, which indicates that new infections may be disseminated through vomiting.

Cited by 19 publications

References 48 publications

Presence of Helicobacter pylori in drinking water of Karachi, Pakistan

Presence of Helicobacter pylori in drinking water of Karachi, Pakistan

The Helicobacter pylori Autotransporter ImaA (HP0289) Modulates the Immune Response and Contributes to Host Colonization

Comparative Analysis of Two Helicobacter pylori Strains using Genomics and Mass Spectrometry-Based Proteomics

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