Presence of high-molecular-weight forms and domain alterations of fibronectin in pleural effusion of patients with lung cancer

Identification of Soluble Supramolecular FN-fibrin Complexes in Human Plasma

“…[18,19] For example, a super FN, an artificial macromolecular construct of a small N-terminal fragment of FN with dimeric FN, showed potent antiangiogenic activity, [13] while a tetrameric form of FN lacked the ability to bind tightly to gelatin and heparin. [19] Moreover, the high-molecular FN reduced forms showing molecular masses of 280 kDa and 320 kDa, reported by us previously, were generally absent in normal human plasma, but they appeared in some cases in plasma of patients with vascular dementia, [20] lung cancer and inflammation, [21] autoimmunization disease, [22] and aged individuals. [23] In the present article, soluble supramolecular plasma FN forms with low electrophoretic mobilities and a tendency to aggregate were analyzed by SDS-agarose FN immunoblotting performed under non-reducing and semidenaturing gel conditions, using capillary transfer of proteins onto nitrocellulose membrane, and detection of FN bands by a specific monoclonal antibody.…”

Molecular Status of Plasma Fibronectin as an Additional Biomarker for Assessment of Alzheimer’s Dementia Risk

“…The appearance of the high-molecular-weight forms of FN has also been reported in diseases other than dementia [16,17] . The diverse composition of the molecular forms of FN was found to be associated with lung cancer and, to a significantly lesser degree, with lung inflammation [16] .…”

“…The diverse composition of the molecular forms of FN was found to be associated with lung cancer and, to a significantly lesser degree, with lung inflammation [16] . According to Toyoshima et al [17] , the 315-kDa form of FN found in the plasma of salivary gland carcinoma patients partly resulted from the expression of alternatively spliced (ED-A, ED-B, and IIICS domains) segments and partly from glycosylation with both Olinked and N-linked oligosaccharide chains.…”

Di(p-methoxyphenyl)amine end-capped tri(p-thiophenylphenyl)amine based molecular glasses as hole transporting materials for solid-state dye-sensitized solar cells

“…NMR ( 1 H & 13 C) spectra were recorded on a Bruker DPX-250 FT-NMR spectrometer. Chemical shis 18 are given in ppm using the residual solvent signal as internal reference. High resolution mass spectrometry was performed by the Small Molecule Mass Spectrometry platform of IMAGIF (Centre de Recherche de Gif).…”