Presence of Hemolysin in Cultures of Pathogenic Leptospires

Alexander, A. D.; Smith, Oliver H.; Hiatt, C. W.; Gleiser, Chester A.

doi:10.3181/00379727-91-22214

Cited by 32 publications

(16 citation statements)

References 2 publications

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“…In 1956, Alexander et al [14] , for the first time, discovered that L interrogans had a hemolysin-like substance that could hemolyze red blood cells of ruminants, such as sheep, cows, and goats. Its hemolytic activity was completely lost after being heated at 56 °C for 5 min, indicating its heat-labile character.…”

Section: Discussionmentioning

confidence: 99%

Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai1

Zhang

Geng

et al. 2005

Acta Pharmacologica Sinica

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Aim: To identify and classify all potential hemolysin candidates of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Methods: All of the potential hemolysin encoding genes were characterized in silico. These genes were cloned and expressed in Escherichia coli. The hemolytic activities of the expressed proteins were assayed observing the hemolysis on sheep blood agar plates. Sphingomyelinase activities of the hemolysin candidates were measured by thin-layer chromatography (TLC) and HPLC for sphingomyelin-hydrolysis. Expression and secretion of the hemolysins in L interrogans were studied by reverse transcription polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assays. Results and Conclusion: The hemolytic activities of hemolysin candidates (LA0327, LA0378, LA1027, LA1029, LA1650, LA3050, LA3937, LA4004) from L interrogans strain Lai were confirmed. They were further divided into two groups, sphingomyelinase hemolysins and non-sphingomyelinase hemolysins, based on their ability to hydrolyze sphingomyelin. Most of these hemolysins were actually expressed in living L interrogans and some of them were secreted into the environment. This study establishes an essential and complete basis for further studying the contribution of hemolysins to the pathogenesis of L interrogans.

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Section: Discussionmentioning

confidence: 99%

Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai1

Zhang

Geng

et al. 2005

Acta Pharmacologica Sinica

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“…The hemolytic activity of these toxins was reported as early as 1956 [Alexander et al, 1956]. Later, several researchers implicated these molecules in the pathogenesis of the disease [Bernheimer and Bey, 1986;del Real et al, 1989;Kasarov, 1970;Segers et al, 1990;Thompson and Manktelow, 1989].…”

Section: Leptospiral Hemolysinsmentioning

confidence: 99%

Insights into Leptospirosis, a Neglected Disease

Sritharan¹

2012

Zoonosis

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“…Supernatants obtained from cultures of some pathogenic leptospires are known to contain a hemolysin (1,20). As long ago as 1959 it was suggested that the hemolysin might be a phospholipase (18).…”

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confidence: 99%