Presence of Helicobacter suis on pork carcasses

Cooman, Lien De; Houf, Kurt; Smet, Annemieke; Flahou, Bram; Ducatelle, Richard; Bruyne, Ellen De; Pasmans, Frank; Haesebrouck, Freddy

doi:10.1016/j.ijfoodmicro.2014.06.016

Cited by 21 publications

(20 citation statements)

References 24 publications

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“…There was a mean of 2804 OTUs in pre‐gastric samples (Table 1). Mouth swabs are commonly used to sample for specific zoonotic and veterinary pathogens (McAllister et al, 2006; De Cooman et al, 2014), and data were examined to determine the viability of swabs as a useful overview of beef pre‐gastric bacterial communities. At the phylum level, proteobacteria were overrepresented in the swab compared with pooled prefermentation samples (57% vs. 38%) with concurrent declines in firmicutes and bacteroidetes.…”

Section: Resultsmentioning

confidence: 99%

Tracking Bacteria through the Entire Gastrointestinal Tract of a Beef Steer

Durso¹,

Miller²,

Schmidt

et al. 2017

Agricultural & Env Letters

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Core Ideas 50–80% of operational taxonomic units can be attributed to the GIT's upstream compartment. Rumen and feces are dominated by firmicutes, 70% of which are in both compartments. Abomasum, small‐, and large‐intestine samples each had distinct taxonomic composition. Ingesta had more proteobacteria and bacteroidetes; tissue had more clostridia. One‐third of the OTUs in the pre‐gastric samples were also found in the rectum. Biological processes within beef cattle gastrointestinal tracts (GIT) influence animal health, and the output (feces) is an important vector for zoonotic pathogens and antibiotic resistant bacteria. Historically, outside of the rumen, little attention was paid to the bacteria along the GIT, despite their essential role in catabolizing feed into feces. Here we characterize bacteria from 15 GIT sites within a beef steer and examine the proportion of bacteria contributed by upstream compartments. This animal displayed characteristic differences between tissue and digesta communities in gastric and large‐intestine, but not small‐intestine, samples. The GIT sites shared between 50 and 80% of the operational taxonomic units (OTUs) with their neighboring upstream compartment, with the exception of the ileum. The ileum shared only 11% with the jejunum but displayed a similar phylum‐level taxonomic distribution with the jejunum. It is unclear whether the observed changes between compartments represent a nonrandom decrease in bacterial number or rapid multiplication of certain taxa.

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Section: Resultsmentioning

confidence: 99%

Tracking Bacteria through the Entire Gastrointestinal Tract of a Beef Steer

Durso¹,

Miller²,

Schmidt

et al. 2017

Agricultural & Env Letters

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“…H. suis is the most prevalent gastric NHPH in humans. Pigs are the natural host of this bacterium, with prevalences reaching 90% or more [ 10 ] and most likely, pigs and possibly also pork are the main sources of human H. suis infection [ 4 , 11 - 13 ].…”

Section: Introductionmentioning

confidence: 99%

Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections

Zhang

Ducatelle

Bruyne

et al. 2015

Vet Res

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Helicobacter (H.) suis can colonize the stomach of pigs as well as humans, causing chronic gastritis and other gastric pathological changes including gastric ulceration and mucosa-associated lymphoid tissue (MALT) lymphoma. Recently, a virulence factor of H. suis, γ-glutamyl transpeptidase (GGT), has been demonstrated to play an important role in the induction of human gastric epithelial cell death and modulation of lymphocyte proliferation depending on glutamine and glutathione catabolism. In the present study, the relevance of GGT in the pathogenesis of H. suis infection was studied in mouse and Mongolian gerbil models. In addition, the relative importance of H. suis GGT was compared with that of the H. pylori GGT. A significant and different contribution of the GGT of H. suis and H. pylori was seen in terms of bacterial colonization, inflammation and the evoked immune response. In contrast to H. pyloriΔggt strains, H. suisΔggt strains were capable of colonizing the stomach at levels comparable to WT strains, although they induced significantly less overall gastric inflammation in mice. This was characterized by lower numbers of T and B cells, and a lower level of epithelial cell proliferation. In general, compared to WT strain infection, ggt mutant strains of H. suis triggered lower levels of Th1 and Th17 signature cytokine expression. A pronounced upregulation of B-lymphocyte chemoattractant CXCL13 was observed, both in animals infected with WT and ggt mutant strains of H. suis. Interestingly, H. suis GGT was shown to affect the glutamine metabolism of gastric epithelium through downregulation of the glutamine transporter ASCT2.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-015-0163-6) contains supplementary material, which is available to authorized users.

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“…Several studies showed that living in close proximity to as well as intense contact with pigs, cats, and dogs leads to a significant risk of gastric NHPH infection . Helicobacter DNA has been detected in saliva from cats, dogs, and pigs, indicating that the oral cavity of these animals may act as source of NHPH infection for humans . Moreover, a pig veterinarian suffering from reflux oesophagitis and dyspepsia showed the presence of H. suis colonization and inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…Fecal‐oral transmission has also been suggested as a possible route for infection in cats . Apart from direct contact, H. suis presence was demonstrated on pork carcasses and in commercial pork, and the bacterium was able to survive for at least 48 hours in minced pork, indicating that handling or consumption of raw or undercooked pork might be a source of human infection as well. An additional transmission route might be contaminated water, as Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%