Presence of Helicobacter pylori in supragingival dental plaque of individuals with periodontal disease and upper gastric diseases

Silva, Denise G.; Stevens, R. H.; Macedo, Jacyara Maria Brito; Albano, Rodolpho Mattos; Falabella, Márcio Eduardo Vieira; Fischer, Ricardo Guimarães; Veerman, E.C.I.; Tinoco, Eduardo Muniz Barretto

doi:10.1016/j.archoralbio.2010.06.018

Cited by 44 publications

(45 citation statements)

References 37 publications

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“…Most published studies have aimed to investigate the oral cavity as a source for subsequent gastric infections, which, of course, is an important issue in this field of research (Namiot et al, 2010;Chaudhry et al, 2011;Silva et al, 2010;de Souza Gonçalves et al, 2009;Bago et al, 2011;Eskandari et al, 2010). In contrast to the results presented here, many studies clearly reported the occurrence of H. pylori in dental plaque and concluded that there was an association between gastroesophageal disease and dental oral status (Silva et al, 2009(Silva et al, , 2010; Morales-Espinosa et al, …”

Section: Discussioncontrasting

confidence: 53%

“…However, until now, no comprehensible cultivation of H. pylori has been reported from any of the different compartments of the oral cavity, independent of its occurrence in the stomach (Al-Ahmad et al, 2010). In this context, associations of the prevalence of H. pylori with oral hygiene and poorly defined dental parameters such as plaque index (PI) and gingival bleeding have been suggested (Silva et al, 2010). Moreover, it has been proposed that periodontal treatment in combination with systemic therapy is helpful in eradicating H. pylori and preventing the recurrence of infection in the stomach (Zarić et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Is Helicobacter pylori resident or transient in the human oral cavity?

Al‐Ahmad

Kürschner

Weckesser

et al. 2012

Journal of Medical Microbiology

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Helicobacter pylori colonizes the stomachs of at least half of the world's human population. The role of the oral cavity in this colonization is not clear and there are, to date, no comprehensive data that clearly demonstrate the isolation of this bacterium from the oral cavity. The aim of this study was to evaluate the prevalence of H. pylori in the oral cavity of 15 patients who tested positive for H. pylori. A comprehensive dental examination of all patients was conducted. Samples were taken from supragingival and subgingival plaque, saliva, periapical exudates and tongue swabs. All samples were taken before the application of antibiotics. A total of 163 oral samples were investigated by PCR using two different H. pylori-specific primer pairs. A PCR inhibition control using a modified plasmid was always included for the most specific primer pair. In addition, a culture technique was used to confirm PCR results. Despite a PCR detection limit of 10 2 bacteria ml "1, out of 14 patients, H. pylori could not be detected in any of the samples taken. In one patient, H. pylori-positive PCR signals were obtained in two samples using only one primer pair. H. pylori could not be cultivated from these two PCR-positive samples; therefore, no correlation to oral colonization status could be established. This study challenges the misleading preconception that H. pylori resides in the human oral cavity and suggests that this bacterium should be considered transient and independent of the oral status. To date, positive PCR results for H. pylori in the oral cavity have been overestimated and not critically interpreted in literature.

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Section: Discussioncontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Is Helicobacter pylori resident or transient in the human oral cavity?

Al‐Ahmad

Kürschner

Weckesser

et al. 2012

Journal of Medical Microbiology

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“…15 According to other researchers, PCR DNA analysis revealed H. pylori in the oral cavity in 3-89% of cases. 4,7,8,14,16 Such disparate results may be due to different procedures employed for sampling, or a consequence of the primers used. This is because microorganisms similar in shape as well as other urease-producing bacteria may in fact be present in complex biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…18,19 The research team of Silva et al demonstrated a correlation between the colonization of supragingival plaque and oral health parameters -specifically, between the percentage of the surface covered with dental plaque and the bleeding rates of periodontal pockets. 16 Patients with higher values of these 2 indicators were likely to report the presence of H. pylori in their oral cavities. The authors also demonstrated a 98% DNA sequence identity in gastric, saliva and plaque bacteria.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of selected oral health indicators and cariogenic bacteria titer in patients with Helicobacter pylori

Urban¹,

Koszowski²,

Płachetka³

et al. 2017

Adv Clin Exp Med

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“…pylori is an important factor in the pathogenesis of numerous diseases including gastro-intestinal, metabolic, autoimmune, vascular and systemic disorders (atherosclerosis, Raynaud's disease; headache and migraine; artritis, immune thrombocytopenia, henoch-Schönlein disease, Sjögren syndrom, autoimmune thyroiditis; rosacea, chronic urticaria, atopic dermatitis, alopecia areata, iron deficiency anemia, amenorrhea, halitosis, oral ulcer or aphtosis) (3,5,16,17,19,22).…”

Section: Introductionmentioning

confidence: 99%