Presence of heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli E308

Yang, Sheng; Deng, Wenwen; Liu, Shuliang; Yu, Xiumei; Mustafa, Ghulam Raza; Chen, Shujuan; He, Li; Ao, Xiaolin; Yang, Yong; Zhou, Kang; Li, Bei; Han, Xinfeng; Xu, Xuebin; Zou, Likou

doi:10.1016/j.jgar.2020.01.009

Cited by 53 publications

(43 citation statements)

References 39 publications

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“…To reduce the use of colistin and other antibiotics in food producing species, the use of heavy metals in feed has been promoted, such as zinc oxide in piglets already before 2013 (ANSES, 2013). A gene encoding resistance to this heavy metal is likely to be found in Salmonella in the coming years, as is already the case for E. coli (Ghazisaeedi et al, 2020;Yang et al, 2020).…”

Section: Discussionmentioning

confidence: 85%

The Spatiotemporal Dynamics and Microevolution Events That Favored the Success of the Highly Clonal Multidrug-Resistant Monophasic Salmonella Typhimurium Circulating in Europe

et al. 2021

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The European epidemic monophasic variant of Salmonella enterica serovar Typhimurium (S. 1,4,[5],12:i:-) characterized by the multi locus sequence type ST34 and the antimicrobial resistance ASSuT profile has become one of the most common serovars in Europe (EU) and the United States (US). In this study, we reconstructed the time-scaled phylogeny and evolution of this Salmonella in Europe. The epidemic S. 1,4,[5],12:i:- ST34 emerged in the 1980s by an acquisition of the Salmonella Genomic Island (SGI)-4 at the 3′ end of the phenylalanine phe tRNA locus conferring resistance to copper and arsenic toxicity. Subsequent integration of the Tn21 transposon into the fljAB locus gave resistance to mercury toxicity and several classes of antibiotics used in food-producing animals (ASSuT profile). The second step of the evolution occurred in the 1990s, with the integration of mTmV and mTmV-like prophages carrying the perC and/or sopE genes involved in the ability to reduce nitrates in intestinal contents and facilitate the disruption of the junctions of the host intestinal epithelial cells. Heavy metals are largely used as food supplements or pesticide for cultivation of seeds intended for animal feed so the expansion of the epidemic S. 1,4,[5],12:i:- ST34 was strongly related to the multiple-heavy metal resistance acquired by transposons, integrative and conjugative elements and facilitated by the escape until 2011 from the regulatory actions applied in the control of S. Typhimurium in Europe. The genomic plasticity of the epidemic S. 1,4,[5],12:i:- was demonstrated in our study by the analysis of the plasmidome. We were able to identify plasmids harboring genes mediating resistance to phenicols, colistin, and fluoroquinolone and also describe for the first time in six of the analyzed genomes the presence of two plasmids (pERR1744967-1 and pERR2174855-2) previously described only in strains of enterotoxigenic Escherichia coli and E. fergusonii.

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Section: Discussionmentioning

confidence: 85%

The Spatiotemporal Dynamics and Microevolution Events That Favored the Success of the Highly Clonal Multidrug-Resistant Monophasic Salmonella Typhimurium Circulating in Europe

et al. 2021

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“…Heavy-metal- and detergent/disinfectant-resistance genes are often carried by the same MGEs harbouring ARGs, sparking concern and growing evidence that selective pressures induced by their extensive use in industry, agriculture and health-care facilities, as well as via contamination, can co-select for AMR [92–94]. The hospital wherein our isolates originated caters for a catchment area significantly associated with agriculture, including food animals (bovine, sheep, pig and poultry), and various crops, such as fruits and nuts [95].…”

Section: Resultsmentioning

confidence: 99%

“…Silver- and copper-resistance genes silA and pcoA were present in only three isolates (4 %; all ST216) and tellurium-resistance gene terA was observed in a single ST200. Heavy-metal-resistance genes, including zntA , merA , pcoA and chrA , are known to be significantly associated with detergent resistance genes, and sul , tet, bla TEM , bla SHV and bla CTX variants [ 93 ]. Furthermore, chromium resistance has been positively correlated with relative bla CTX-M and bla OXA gene abundance [ 96 ].…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis of trimethoprim-resistant extraintestinal pathogenic Escherichia coli and recurrent urinary tract infections

Reid

Kudinha

et al. 2020

Microbial Genomics

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Urinary tract infections (UTIs) are the most common bacterial infections requiring medical attention and a leading justification for antibiotic prescription. Trimethoprim is prescribed empirically for uncomplicated cases. UTIs are primarily caused by extraintestinal pathogenic Escherichia coli (ExPEC) and ExPEC strains play a central role in disseminating antimicrobial-resistance genes worldwide. Here, we describe the whole-genome sequences of trimethoprim-resistant ExPEC and/or ExPEC from recurrent UTIs (67 in total) from patients attending a regional Australian hospital from 2006 to 2008. Twenty-three sequence types (STs) were observed, with ST131 predominating (28 %), then ST69 and ST73 (both 7 %). Co-occurrence of trimethoprim-resistance genes with genes conferring resistance to extended-spectrum β-lactams, heavy metals and quaternary ammonium ions was a feature of the ExPEC described here. Seven trimethoprim-resistance genes were identified, most commonly dfrA17 (38 %) and dfrA12 (18 %). An uncommon dfrB4 variant was also observed. Two blaCTX-M variants were identified – blaCTX-M-15 (16 %) and blaCTX-M-14 (10 %). The former was always associated with dfrA12, the latter with dfrA17, and all blaCTX-M genes co-occurred with chromate-resistance gene chrA. Eighteen class 1 integron structures were characterized, and chrA featured in eight structures; dfrA genes featured in seventeen. ST131 H30Rx isolates possessed distinct antimicrobial gene profiles comprising aac(3)-IIa, aac(6)-Ib-cr, aph(3′)-Ia, aadA2, blaCTX-M-15 , blaOXA-1 and dfrA12. The most common virulence-associated genes (VAGs) were fimH, fyuA, irp2 and sitA (all 91 %). Virulence profile clustering showed ST131 H30 isolates carried similar VAGs to ST73, ST405, ST550 and ST1193 isolates. The sole ST131 H27 isolate carried molecular predictors of enteroaggregative E. coli /ExPEC hybrid strains (aatA, aggR, fyuA). Seven isolates (10 %) carried VAGs suggesting ColV plasmid carriage. Finally, SNP analysis of serial UTI patients experiencing worsening sequelae demonstrated a high proportion of point mutations in virulence factors.

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“…In addition to ARGs, DRGs and HMRGs were commonly present among these isolates, and DRGs and HMRGs often co-existed with ARGs in MDR plasmids. A recent report by Yang [ 41 ] showed that up to 87% of Salmonella isolated from broiler chicken farms and retail meats in China carried HMRGs. Additionally, the HMRGs were significantly associated with the presence of DRGs and ARGs [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…A recent report by Yang [ 41 ] showed that up to 87% of Salmonella isolated from broiler chicken farms and retail meats in China carried HMRGs. Additionally, the HMRGs were significantly associated with the presence of DRGs and ARGs [ 41 ]. The significant association between HMRGs with DRGs and ARGs implicated that widely using disinfectants and heavy metals in animal husbandry and food production may play a critical role in co-selecting for antimicrobial resistance development and dissemination.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Analysis of 450 Strains of Salmonella enterica Isolated from Diseased Animals

Zhao

Liu

Hsu

et al. 2020

Genes

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Salmonella is a leading cause of bacterial infections in animals and humans. We sequenced a collection of 450 Salmonella strains from diseased animals to better understand the genetic makeup of their virulence and resistance features. The presence of Salmonella pathogenicity islands (SPIs) varied by serotype. S. Enteritidis carried the most SPIs (n = 15), while S. Mbandaka, S. Cerro, S. Meleagridis, and S. Havana carried the least (n = 10). S. Typhimurium, S. Choleraesuis, S. I 4,5,12:i:-, and S. Enteritidis each contained the spv operon on IncFII or IncFII-IncFIB hybrid plasmids. Two S. IIIa carried a spv operon with spvD deletion on the chromosome. Twelve plasmid types including 24 hybrid plasmids were identified. IncA/C was frequently associated with S. Newport (83%) and S. Agona (100%) from bovine, whereas IncFII (100%), IncFIB (100%), and IncQ1 (94%) were seen in S. Choleraesuis from swine. IncX (100%) was detected in all S. Kentucky from chicken. A total of 60 antimicrobial resistance genes (ARGs), four disinfectant resistances genes (DRGs) and 33 heavy metal resistance genes (HMRGs) were identified. The Salmonella strains from sick animals contained various SPIs, resistance genes and plasmid types based on the serotype and source of the isolates. Such complicated genomic structures shed light on the strain characteristics contributing to the severity of disease and treatment failures in Salmonella infections, including those causing illnesses in animals.

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Presence of heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli E308

Cited by 53 publications

References 39 publications

The Spatiotemporal Dynamics and Microevolution Events That Favored the Success of the Highly Clonal Multidrug-Resistant Monophasic Salmonella Typhimurium Circulating in Europe

The Spatiotemporal Dynamics and Microevolution Events That Favored the Success of the Highly Clonal Multidrug-Resistant Monophasic Salmonella Typhimurium Circulating in Europe

Genomic analysis of trimethoprim-resistant extraintestinal pathogenic Escherichia coli and recurrent urinary tract infections

Comparative Genomic Analysis of 450 Strains of Salmonella enterica Isolated from Diseased Animals

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