Presence of estrogen receptor (ER)-like proteins and endogenous ligands for ER in solanaceae

Milanesi, Lorena Magdalena; Boland, Ricardo

doi:10.1016/j.plantsci.2003.10.006

Cited by 20 publications

(24 citation statements)

References 28 publications

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“…Yang et al [36] characterized a putative Membrane Steroid Binding Protein (MSBP1) that binds progesterone with high affinity and functions in regulating growth in Arabidopsis. Estrogen binding proteins were reported in Solanaceae [37] and steroid binding proteins specific for progesterone and 17β-estradiol were reported in Triticum aestivum [38]. Other research implies that the stimulating effects …”

Section: Growth and Developmentmentioning

confidence: 99%

Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana

Upadhyay¹,

Maier²

2016

AJPS

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Mammalian sex hormones are spread in the environment from natural and anthropogenic sources. In the present study, the effect of estradiol on Arabidopsis thaliana growth primary metabolism, phenylpropanoid and flavonoid pathways and pathogen resistance were investigated. Treatments of Arabidopsis plants with 10 and 100 nM 17β-estradiol resulted in enhanced root growth and shoot biomass. In addition, treated plants had an increased rate of photosynthesis with a concomitant increase in carbohydrate and protein accumulation. Plants exposed to higher concentrations of 17β-estradiol (10 μM) had significantly lower root growth, biomass, photosynthesis rate, primary metabolite and phenylpropanoid and flavonoid contents indicating a toxic effect of estradiol. Treatments with increasing estradiol concentrations (10 nM, 100 nM and 10 μM) resulted in the downregulation of phenylpropanoid-flavonoid pathway genes (PAL1, PAL4, CHI and ANS) and subsequent decreased accumulation of phenolics, flavonoids and anthocyanins. Estradiol-treated plants were inoculated with Pseudomonas syringae pv. Tomato DC3000 and basal resistance was determined. Estradiol treatments rendered plants susceptible to the pathogen, thus compromising the plant defense mechanisms. These results indicate that at low concentrations, estradiol functions as a biostimulant of growth, yield and primary metabolism of Arabidopsis. However, estradiol functions as a potential transcriptional regulator of the phenylpropanoid pathway genes in Arabidopsis, having a negative effect on the phenylpropanoid and flavonoid biosynthetic pathways.

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Section: Growth and Developmentmentioning

confidence: 99%

Effects of 17β-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in Arabidopsis thaliana

Upadhyay¹,

Maier²

2016

AJPS

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“…These plant compounds have got structural similarities with mammalian 17β-estradiol (E2) and are capable of binding to estrogen receptors (ERs) [5][6][7]. Even though phytoestrogens can activate ERs, their effects are approximately thousand folds weaker than E2 [8].…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned, the synthesis of molecules structurally and functionally related to the mammalian steroid hormones by plants, has been reported [5,6,10,11]. However, their physiological role in plants is controversial.…”

Section: Introductionmentioning

confidence: 99%

“…(Solanum malacoxylon Send. ), Lycopersicon esculentum (Mill) and Nicotiana glauca (Graham) by competition assays using specific polyclonal antibodies against the respective mammalian steroid hormones [5,6,12]. Binding experiments with [ 3 H]17β-estradiol in the presence or absence of an excess of the unlabelled steroid showed that the Solanaceae organs contain estrogen binding sites.…”

Section: Introductionmentioning

confidence: 99%

“…The protein nature of these sites was clearly indicated by their sensitivity to trypsin degradation. Likewise, lipid crude extracts from Solanaceae were able to compete with [ 3 H]17β-estradiol for binding to the estrogen receptor (ER) from breast cancer MCF-7 cells as well as with estrogen binding sites present in this plant species [5,6,12].…”

Section: Introductionmentioning

confidence: 99%

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EXTRACTS FROM Nicotiana glauca INDUCE APOPTOSIS THROUGH CASPASES IN SKELETAL MUSCLE CELLS

Lincor

Musso

Vasconsuelo

et al. 2017

Proceedings of the 21st International Electronic Conference on Synthetic Organic Chemistry

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Phytoestrogens are plant compounds which have generated considerable interests. A litany of health benefits including a lowered risk of osteoporosis, breast cancer, and menopausal symptoms, are frequently attributed to phytoestrogens but without the knowledge of their side effects. We investigate the effects of lipid extracts from the Solanaceae Nicotiana glauca on skeletal muscle cells, in relation to apoptosis. Opposite to the effects of 17β-estradiol, the crude extract from N. glauca and its sub-extracts induced apoptosis in C2C12 cells. This apoptotic action involved caspase 3/7 activation. These data suggest that the traditional use of this medicinal plant could affect the skeletal muscle homeostasis.

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Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures

Huang

Plesha

McDonald

2010

Biotech & Bioengineering

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Plant cell culture is an alternative for the production of recombinant human therapeutic proteins because of improved product safety, lower production cost, and capability for eukaryotic post-translational modification. In this study, bioreactor production of recombinant human alpha-1-antitrypsin (rAAT) glycoprotein using a chemically inducible Cucumber mosaic virus (CMV) viral amplicon expression system in transgenic Nicotiana benthamiana cell culture is presented. Optimization of a chemically inducible plant cell culture requires evaluation of effects of timing of induction (TOI) and concentration of inducer (COI) on protein productivity and protein quality (biological functionality). To determine the optimal TOI, the oxygen uptake rate (OUR) of the plant cell culture was chosen as a physiological indicator for inducing maximum rAAT expression. Effects of COI on rAAT production were investigated using a semicontinuous culture, which enables the distinction between effects of growth rate and effects of inducer concentration. An optimized semicontinuous bioreactor operation was further proposed to maximize the recombinant protein production. The results demonstrated that the transgenic plant cells, transformed with the inducible viral amplicon expression system, maintain higher OUR and exhibit lower extracellular protease activity and lower total phenolics concentration in the optimized semicontinuous bioreactor process than in a traditional batch bioreactor operation, resulting in a 25-fold increase in extracellular functional rAAT (603 microg/L) and a higher ratio of functional rAAT to total rAAT (85-90%). Surprisingly, sustained rAAT production and steady state, long-term bioreactor operation is possible following chemical induction and establishment of the viral amplicons.

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Presence of estrogen receptor (ER)-like proteins and endogenous ligands for ER in solanaceae

Cited by 20 publications

References 28 publications

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

EXTRACTS FROM <em>Nicotiana glauca</em> INDUCE APOPTOSIS THROUGH CASPASES IN SKELETAL MUSCLE CELLS

Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures

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Presence of estrogen receptor (ER)-like proteins and endogenous ligands for ER in solanaceae

Cited by 20 publications

References 28 publications

Effects of 17&lt;i&gt;β&lt;/i&gt;-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Effects of 17&lt;i&gt;β&lt;/i&gt;-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

EXTRACTS FROM <em>Nicotiana glauca</em> INDUCE APOPTOSIS THROUGH CASPASES IN SKELETAL MUSCLE CELLS

Semicontinuous bioreactor production of a recombinant human therapeutic protein using a chemically inducible viral amplicon expression system in transgenic plant cell suspension cultures

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Product

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Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>

Effects of 17<i>β</i>-Estradiol on Growth, Primary Metabolism, Phenylpropanoid-Flavonoid Pathways and Pathogen Resistance in <i>Arabidopsis thaliana</i>