Presence of enteroviruses in recreational water in Wuhan, China

Allmann, Erin; Pan, Lei; Li, Lü; Li, Dejia; Wang, Haihui; Lu, Yuanan

doi:10.1016/j.jviromet.2013.06.028

Cited by 20 publications

(12 citation statements)

References 28 publications

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“…However, it is not possible for all microorganisms, particularly nonculturable viruses. This difficulty in interpretation is exemplified by the high prevalence of human norovirus which has been obtained by RT‐qPCR in different matrices: 3.9% to 59.1% in shellfish (Lowther and others ; Woods and Burkhardt ), 4.2% to 45% in water (Lodder and others ; Borchardt and others ; Allmann and others ), 23.3% in cherry tomatoes (Stals and others ), 6.6% to 40.0% in soft red fruits (Baert and others ; Stals and others ), and 28.2% to 50.0% in leafy greens (Baert and others ). Improper interpretation of such data can lead to misconceptions of public health risk.…”

Section: Interpretation Of Molecular Analytical Methodsmentioning

confidence: 99%

Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection

Ceuppens

Uyttendaele

et al. 2014

Comp Rev Food Sci Food Safe

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Because of increasing demand for rapid results, molecular techniques are now applied for the detection of microorganisms in foodstuffs. However, interpretation problems can arise for the results generated by molecular methods in relation to the associated public health risk. Discrepancies between results obtained by molecular and conventional culture methods stem from the difference in target, namely nucleic acids instead of actively growing microorganisms. Nucleic acids constitute 5% to 15% of the dry weight of all living cells and are relatively stable, even after cell death, so they may be present in a food matrix after the foodborne microorganisms have been inactivated. Therefore, interpretation of the public health significance of positive results generated by nucleic acid detection methods warrants some additional consideration. This review discusses the stability of nucleic acids in general and highlights the persistence of microbial nucleic acids after diverse food-processing techniques based on data from the scientific literature. Considerable amounts of DNA and RNA (intact or fragmented) persist after inactivation of bacteria and viruses by most of the commonly applied treatments in the food industry. An overview of the existing adaptations for molecular assays to cope with these problems is provided, including large fragment amplification, flotation, (enzymatic) pretreatment, and various binding assays. Finally, the negligible risks of ingesting free microbial nucleic acids are discussed and this review ends with the future perspectives of molecular methods such as next-generation sequencing in diagnostic and source attribution food microbiology.

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Section: Interpretation Of Molecular Analytical Methodsmentioning

confidence: 99%

Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection

Ceuppens

Uyttendaele

et al. 2014

Comp Rev Food Sci Food Safe

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“…Organic pollution from municipal wastewater is responsible for microbiological contamination in surface waters (Jamieson et al 2004;Allmann et al 2013). During the 19th century, waterborne diseases were responsible for large epidemics of dysentery, typhoid fever, cholera, among others (Smith 2002).…”

Section: Introductionmentioning

confidence: 99%

Effects of rainfalls variability and physical–chemical parameters on enteroviruses in sewage and lagoon in Yopougon, Côte d’Ivoire

et al. 2016

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The aim of this study was to assess the variability of the content of nutrients, oxidizable organic and particulate matters in raw sewage and the lagoon on the effect of rainfall. Then evaluate the impact of these changes in the concentration of enteroviruses (EVs) in waters. The sewage samples were collected at nine sampling points along the channel, which flows, into a tropical lagoon in Yopougon. Physical-chemical parameters (5-day Biochemical Oxygen Demand, Chemical Oxygen Demand, Suspended Particulate Matter, Total Phosphorus, Orthophosphate, Total Kjeldahl Nitrogen and Nitrate) as well as the concentration of EV in these waters were determined. The average numbers of EV isolated from the outlet of the channel were 9.06 9 10 4 PFU 100 ml -1 . Consequently, EV was present in 55.55 and 33.33 % of the samples in the 2 brackish lagoon collection sites. The effect of rainfall on viral load at the both sewage and brackish lagoon environments is significant correlate (two-way ANOVA, P \ 0.05). Furthermore, in lagoon environment, nutrients (Orthophosphate, Total Phosphorus), 5-day Biochemical Oxygen Demand, Chemical Oxygen Demand and Suspended Particulate Matter were significant correlated with EVs loads (P \ 0.05 by Pearson test). The overall results highlight the problem of sewage discharge into the lagoon and correlation between viral loads and water quality parameters in sewage and lagoon.

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“…In the period 1986-1996, 710 outbreaks of waterborne diseases were reported in nineteen European countries; 65 of these were of viral aetiology (1,11). The largest enteroviral European outbreak occurred in Minsk, Belarus, in the year 2003.…”

Section: Discussionmentioning

confidence: 99%

“…Contaminated drinking, recreational or surface waters which contain enteric viruses such as adenoviruses, rotaviruses, noroviruses, astroviruses, and picornaviruses remain a serious public health problem (1). Recreational-epidemiological studies show that diarrhoea and respiratory infections are commonly reported, and it is believed that these may be associated with a variety of enteric viruses of which some remain unidentified (2).…”

Section: Introductionmentioning

confidence: 99%

Should Enteroviruses Be Monitored in Natural Recreational Waters?

Šarmírová

Nagyová

Stipalova

et al. 2016

Cent Eur J Public Health

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SUMMARYEnteroviruses (EVs) infections occur worldwide. Although, infections by these viruses are often asymptomatic and go unnoticed, they can be shed in stool for several weeks. The EVs are associated with sporadic outbreaks and a wide range of clinical symptoms, occasionally accompanied with fatal consequences. Presently in the Slovak Republic (SR) recreational waters are tested only for bacterial indicators. Our aim was to monitor EVs in recreational waters. Water samples were collected during the years 2012-2014 from different recreational natural lakes in Central and West regions of SR. The samples were concentrated by centrifugation using the two-phase separation method recommended by the World Health Organization (WHO) used for EVs surveillance in the treated sewage waste water. Each of the two phases collected from the samples was analysed by polymerase chain reaction for detection of EVs and primary sequencing was done. Our study demonstrated presence of EVs in three localities consecutively for three years, indicating a probability of constant local source of faecal contamination. This is the first monitoring report on the occurrence of EVs in the natural recreational waters in SR.

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Presence of enteroviruses in recreational water in Wuhan, China

Cited by 20 publications

References 28 publications

Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection

Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection

Effects of rainfalls variability and physical–chemical parameters on enteroviruses in sewage and lagoon in Yopougon, Côte d’Ivoire

Should Enteroviruses Be Monitored in Natural Recreational Waters?

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