Presence of enteric viruses, bioaccumulation and stability in Anomalocardia brasiliana clams (Gmelin, 1791)

Souza, Dóris Sobral Marques; Dominot, Ana Ferreira Ávila; Moresco, Vanessa; Barardi, Célia Regina Monte

doi:10.1016/j.ijfoodmicro.2017.08.004

Cited by 20 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was in agreement with Souza et al (17), who did not detect any NoV GI or NoV GII positive samples in oysters harvested in regular cultivation areas in South Brazil. Our results are also in accordance with those described by Souza et al (19). They collected samples of Anomalocardia brasiliana clams monthly between November 2014 and April 2016 in South Brazil and found only two positive samples for NoV GI and none for NoV GII.…”

Section: Discussionsupporting

confidence: 93%

“…In Brazil, only a few groups had investigated NoV contamination in shellfish in the South and Southeast regions of the country. The detection rate in these studies has varied from 0 to 4.8% ( 17 - 19 ). This study was the first to investigate NoV contamination in shellfish from the Northeast region of the country.…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, a few studies have detected HuNoV in shellfish harvested in South and Southeast Brazil (17)(18)(19). The Northeast region is one of the favorite travel destinations in Brazil because of its stunning coastline and rich seafood cuisine.…”

Section: Introductionmentioning

confidence: 99%

“…There is still much to be elucidated about the biology and ecology of this important foodborne pathogen ( 8 ). In Brazil, a few studies have detected HuNoV in shellfish harvested in South and Southeast Brazil ( 17 - 19 ). The Northeast region is one of the favorite travel destinations in Brazil because of its stunning coastline and rich seafood cuisine.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Absence of norovirus contamination in shellfish harvested and commercialized in the Northeast coast of Brazil

Guarines

Mendes

Cordeiro

et al. 2020

Braz J Med Biol Res

View full text Add to dashboard Cite

Norovirus (NoV) is the main cause of gastroenteritis outbreaks worldwide. Although NoV spreads mainly from person to person, it is estimated that a large proportion of NoV outbreaks are caused by foodborne transmission. Bivalve mollusks are one of the most important foods involved in NoV transmission to humans. Little is known about NoV prevalence in shellfish harvested and commercialized in Brazil. The aim of this study was to map, for the first time, the distribution of NoV contamination in oysters and mussels harvested and commercialized in the coast of Pernambuco state, northeast Brazil. A total of 380 mollusks (260 oysters and 120 mussels) were collected between February and August 2017 either directly from harvesting areas or obtained from beach vendors at 17 sites in Pernambuco. Samples were processed and tested for NoV contamination using a SYBR Green real-time PCR assay. All samples were negative for NoV GI or GII contamination, suggesting a low risk of NoV contamination from this food source during the study period. Additional surveys in different areas of the Brazilian coast are warranted to monitor the risk of NoV infection upon seafood consumption.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Absence of norovirus contamination in shellfish harvested and commercialized in the Northeast coast of Brazil

Guarines

Mendes

Cordeiro

et al. 2020

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Artificial contamination of shellfish with viral pathogens is a common approach to studying depuration processes and has been described previously (Muniain-Mujika et al 2002 ; Choi and Jiang 2005 ; Nappier et al 2008 ; McLeod et al 2009 ; Polo et al 2014b ). As shellfish are known to efficiently bioaccumulate viruses in as little as several hours (Flannery et al 2012 ; Souza et al 2013 , 2018 ; Pilotto et al 2019 ), this approach allows for a rapid generation of animals contaminated with one or multiple viral strains. Concentrations as high as 10 11 genome copies per gram (gcg −1 ) of murine norovirus (MNV1) were achieved during 24h bioaccumulation in C. gigas by Pilotto et al ( 2019 ), whereas both GI and GII human norovirus genogroups, simultaneously bioaccumulated in C. gigas oysters to 10 6 gcg −1 (Maalouf et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Norovirus Reduction in Environmentally Contaminated Pacific Oysters During Laboratory Controlled and Commercial Depuration

et al. 2021

View full text Add to dashboard Cite

Norovirus contamination of oysters is the lead cause of non-bacterial gastroenteritis and a significant food safety concern for the oyster industry. Here, norovirus reduction from Pacific oysters (Crassostrea gigas), contaminated in the marine environment, was studied in laboratory depuration trials and in two commercial settings. Norovirus concentrations were measured in oyster digestive tissue before, during and post-depuration using the ISO 15216-1 quantitative real-time RT-PCR method. Results of the laboratory-based studies demonstrate that statistically significant reductions of up to 74% of the initial norovirus GII concentration was achieved after 3 days at 17–21 °C and after 4 days at 11–15 °C, compared to 44% reduction at 7–9 °C. In many trials norovirus GII concentrations were reduced to levels below 100 genome copies per gram (gcg−1; limit of quantitation; LOQ). Virus reduction was also assessed in commercial depuration systems, routinely used by two Irish oyster producers. Up to 68% reduction was recorded for norovirus GI and up to 90% for norovirus GII reducing the geometric mean virus concentration close to or below the LOQ. In both commercial settings there was a significant difference between the levels of reduction of norovirus GI compared to GII (p < 0.05). Additionally, the ability to reduce the norovirus concentration in oysters to < LOQ differed when contaminated with concentrations below and above 1000 gcg−1. These results indicate that depuration, carried out at elevated (> 11 °C) water temperatures for at least 3 days, can reduce the concentration of norovirus in oysters and therefore consumer exposure providing a practical risk management tool for the shellfish industry.

show abstract

Beneficial microorganisms for the health-promoting in oyster aquaculture: realistic alternatives

Todorov,

Carneiro,

Lipilkina

et al. 2024

Aquacult Int

View full text Add to dashboard Cite

Presence of enteric viruses, bioaccumulation and stability in Anomalocardia brasiliana clams (Gmelin, 1791)

Cited by 20 publications

References 46 publications

Absence of norovirus contamination in shellfish harvested and commercialized in the Northeast coast of Brazil

Absence of norovirus contamination in shellfish harvested and commercialized in the Northeast coast of Brazil

Evaluation of Norovirus Reduction in Environmentally Contaminated Pacific Oysters During Laboratory Controlled and Commercial Depuration

Beneficial microorganisms for the health-promoting in oyster aquaculture: realistic alternatives

Contact Info

Product

Resources

About