Presence of EBNA in nasopharyngeal carcinoma and control patient tissues related to ebv serology

Huang, Dolly P.; Ho, Helen; Henle, Werner; Henle, Gertrude; Saw, Daisy; Lui, Man Tin

doi:10.1002/ijc.2910220308

Cited by 46 publications

(15 citation statements)

References 23 publications

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“…Elevated anti-VCA-IgG and anti-EA-IgG titers have been reported in RA [15], in SLE [16] and in a variety of immunosuppressive condi tions [13,14]. In the case of significantly EBV-reactivated conditions such as Burkitt lymphoma or nasopharyngeal carcinoma (NPC), elevated anti-VCA-IgG and anti-EAIgG titers have been shown to be more pronounced and EBNA titers are also elevated [28]. Anti-VCA-IgA and anti-EA-IgA can be detected only in patients with NPC [29,30] and EBNA titers are low or even undetectable in 452 Yamamoto/Nakajima/Yamamoto/ H ironaka/ H i rai/Naka mura EBV ir.…”

Section: Discussionmentioning

confidence: 99%

Epstein-Barr Virus Activity in Patients on Chronic Hemodialysis

Yamamoto

Nakajima²,

Yamamoto³

et al. 1995

Nephron

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Patients with uremia are susceptible to viral infections, especially to Epstein-Barr virus (EBV). Sixty-one patients with end-stage renal diseases on chronic hemodialysis (HD), 14 patients with impaired renal function (CRF), and 27 healthy controls were studied with regard to EBV infection. Uremic patients (HD and CRF) had a significantly higher incidence of EBV infection and higher titers of anti-EBV VCA-IgG antibody than healthy controls. The anti-EBNA-1 titer was significantly higher in patients whose dialysis period was more than 3 months than in whom the dialysis period was 3 months or less. Immunoblotting analysis also showed stronger EBNA-1 signals in hemodialysis patients than EBNA-2, which was strongly detected in the CRF group and in healthy controls. EBV DNA was detected by Southern blot hybridization after PCR amplification of peripheral leukocytes, and occurred at a greater incidence in hemodialysis patients than in the other groups. Taken together, these results demonstrated that hemodialysis patients had persistent EBV infection.

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Section: Discussionmentioning

confidence: 99%

Epstein-Barr Virus Activity in Patients on Chronic Hemodialysis

Yamamoto

Nakajima²,

Yamamoto³

et al. 1995

Nephron

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“…Monolayer coverslips were examined using the anti-C' immunofluorescence (ACIF) test as described by Reedman and Klein (1973) with modifications by Huang et al (1978). Three anti-EBNA-positive sera and one anti-EBNA-negative serum were used for each determination.…”

Section: Test For Ebnamentioning

confidence: 99%

Establishment of a cell line (NPC/HK1) from a differentiated squamous carcinoma of the nasopharynx

Huang

Poon

et al. 1980

Intl Journal of Cancer

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198

151

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A long-term cell culture epithelioid cell line was established from a recurrent squamous carcinoma of the nasopharynx of a Chinese male 17 1/2 years after radiation therapy. The cell line, designated NPC/HK1, has been passed 72 times over a period 1 year. The cells have been shown by light and electron microscopies to be of the squamous epithelial type. When they were transplanted subcutaneously into the back of athymic nude BALB/c (nu/nu) mice, tumors developed at the sites of inoculation, which on histological examination were shown to be well-differentiated squamous carcinomas, similar in morphology to the recurrent human tumor from which they were derived. Karyotypic analysis of cells from the cell line demonstrates an aneuploid human type with a modal chromosome number of 74 with both numerical and structural aberrations. Viral particles or Epstein-Barr viral nuclear antigen (EBNA) has not been demonstrated in the cells from the primary culture or several of the subcultures tested. The presence of EBNA in touch smears prepared from the biopsy tissue was inconclusive. Infection of the subcultured cells with EBV from P3HR1 and B95-8 cells was unsuccessful.

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“…The patients were retrospectively staged according to the classification of Dr. J.H.C. Ho (1978) with the additional clinical importance that T3 features were staged as C instead of B. Initial treatment consisted of a combination of radiation therapy and chemotherapy.…”

Section: Patientsmentioning

confidence: 99%

“…Undifferentiated or nonkeratinizing, but not welldifferentiated, carcinomas of the postnasal space are intimately associated with the Epstein-Barr virus (EBV). Viral DNA can be frequently demonstrated in biopsy specimens (zur Hausen et al, 1970;Nonoyama et al, 1973;Desgranges et al, 1975;Anderson-Anvret et al, 1977, 1979Lanier et al, 1978Lanier et al, , 1981, and the carcinoma cells, but not the lymphocytes present in the tumor, contain EBV DNA and express the EBVassociated nuclear antigen (EBNA) (Wolf et al, 1973(Wolf et al, , 1975Klein et a/., 1974;Huang et al, 1974Huang et al, , 1978. With increasing tumor burden, patients with NPC develop a broad spectrum of circulating antibodies to EBV-specific antigens that are highly characteristic of the disease.…”

mentioning

confidence: 99%

Nasopharyngeal carcinoma in american children: Epstein‐Barr virus‐specific antibody titers and prognosis

Naegele

Champion

Murphy

et al. 1982

Intl Journal of Cancer

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Seven American juvenile patients with undifferentiated or nonkeratinizing nasopharyngeal carcinoma (NPC) were examined serially for Epstein-Barr virus (EBV)-specific antibody spectra and titers in sera. At diagnosis, all showed antibody patterns characteristic of NPC: i.e., high titers of IgG antibodies to viral capsid antigen (VCA) and to the diffuse (D) component of the early antigen complex. Six patients had IgA antibodies to VCA, and four to the D component. In the patients who responded to therapy with complete and maintained remissions, the IgG antibodies to D and the IgA antibodies to VCA and D decreased to undetectable levels within 12 to 30 months. By contrast, of the four patients who responded only transiently to therapy, three showed substantial increases and one continuously high titers of IgG anti-D and IgA anti-VCA. The increases in antibody titers preceded clinical recognition of recurrent tumors by 1 to 6 months. Three of these patients have died and the fourth is alive with disease. These data indicate that American juvenile NPC does not differ from the adult disease observed anywhere in the World. They reaffirm the potential usefulness of EBV-specific serology in the diagnosis and prognosis of NPC and the monitoring of patients following therapy.

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Presence of EBNA in nasopharyngeal carcinoma and control patient tissues related to ebv serology

Abstract: A search was made for Epstein-BARR virus (EBV) associated nuclear antigen (EBNA) in touch preparations of fresh biopsy material f r o m 26 carcinomas of the nasopharynx, 39 other tumours of the head and neck. the nasopharyngeal mucosa of 32 patients

Cited by 46 publications

References 23 publications

Epstein-Barr Virus Activity in Patients on Chronic Hemodialysis

Epstein-Barr Virus Activity in Patients on Chronic Hemodialysis

Establishment of a cell line (NPC/HK1) from a differentiated squamous carcinoma of the nasopharynx

Nasopharyngeal carcinoma in american children: Epstein‐Barr virus‐specific antibody titers and prognosis

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