Presence of dendroaspis natriuretic peptide and its binding to NPR-A receptor in rabbit kidney

Kim, Soo Mi; Kim, Yoon Ah; Kim, Sun Young; Kim, Suhn Hee; Cho, Kyung Woo; Kim, Sung Zoo

doi:10.1016/j.regpep.2010.11.010

Cited by 5 publications

(12 citation statements)

References 26 publications

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“…Our recent study demonstrated that the concentration of DNP was relatively high in rabbit plasma, while the tissue contents of DNP in various organs, including the liver, spleen, intestine, atrium, ventricle, septum, kidney, cerebrum and cerebellum, were quite low (10). The major sites for DNP synthesis and secretion have not been identified, although the concentration of DNP in the plasma was markedly higher compared with that of ANP in the rabbit.…”

Section: Introductionmentioning

confidence: 95%

“…The major sites for DNP synthesis and secretion have not been identified, although the concentration of DNP in the plasma was markedly higher compared with that of ANP in the rabbit. Notably, the high plasma concentrations of DNP were identified to be correlated with the biological half-life of DNP in the circulation of the rabbit (10). Since the stability of DNP in plasma is a significant factor with regard to its half-life and renal functions, the current experiments were designed to confirm the stability of DNP versus ANP, BNP and CNP in rat plasma, and to determine a physiological target site for the degradation of DNP.…”

Section: Introductionmentioning

confidence: 99%

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Dendroaspis natriuretic peptide is degraded by a metalloproteinase in the rat kidney

Kim

et al. 2013

Molecular Medicine Reports

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Abstract. Our previous study demonstrated that the concentration of dendroaspis natriuretic peptide (DNP) was markedly higher than that of atrial NP (ANP) in rabbit plasma, indicating that DNP has a different metabolic rate from other NPs. Therefore, the metabolic characteristics of DNP in mammals require further analysis. The stabilities of NPs were determined by incubating 125 I-labeled ANP, brain NP (BNP), C-type NP (CNP) and DNP at 37˚C for 1, 2 and 4 h, and analyzing their profiles by reversed-phase high-performance liquid chromatography.125 I-labeled ANP, BNP and CNP were quickly degraded in rat plasma, while 125 I-labeled DNP was stable for 4 h. The relative stability of the peptides following incubation in rat plasma followed the rank order of: DNP>>>ANP≥BNP>>CNP. Organs were also examined for the degradation of DNP, including the spleen, kidney, liver, heart and lung. The physiological target organ for the degradation of DNP was observed to be the kidney. Furthermore, degradation of DNP in the kidney was attenuated by phenanthroline, a metalloproteinase inhibitor. Therefore, these results indicate that DNP has a longer stability in plasma and that it may have strong therapeutic applications in cardiac disease.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Dendroaspis natriuretic peptide is degraded by a metalloproteinase in the rat kidney

Kim

et al. 2013

Molecular Medicine Reports

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“…The animals were maintained at ~20˚C with a constant humidity of 55% and acclimated to standard conditions. The animals were maintained on an ad libitum diet of standard chow and tap water, maintained at 22-23˚C with a constant humidity of 55%, and acclimated with a cycle of 12 h of light/darkness, as described previously (8). The present study was approved by the ethics committee of Chonbuk National University, Institute for Medical Science (Jeonju, Jeonbuk, Republic of Korea), according to the National Institutes of Health (Bethesda, MD, USA) guidelines.…”

Section: Animalsmentioning

confidence: 99%

“…The animals were sacrificed under pentobarbital (30-40 mg/kg) stable anesthesia and by decapitation. The plasma DNP concentrations were determined, as previously described (8). Briefly, blood was collected and plasma samples were obtained by centrifugation at 10,000 × g for 10 min at 4˚C.…”

Section: Animalsmentioning

confidence: 99%

“…The natriuretic peptide receptors (NPRs) for DNP have been reported to localize to the smooth muscle tissue of the human mammary artery (4); in addition, DNP has been observed to induce vasodilatation via the direct activation of these receptors (7). Our previous studies provided evidence that DNP exerts renal actions via the NPR-A subtype and demonstrated that DNP and its specific receptor are present in the kidneys of rabbits (8)(9)(10). Collectively, these results suggested that DNP has a physiological role in regulating the renal function of mammals by interacting with specific NPRs, which have a particular guanylyl cyclase (pGC) domain.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of dendroaspis natriuretic peptide and its receptor in streptozotocin-induced diabetic rats

Park

Kim

et al. 2015

Molecular Medicine Reports

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Dendroaspis natriuretic peptide (DNP) shares a functionally important sequence homology with other natriuretic peptides. However, the characteristics of DNP and its receptor in the context of diabetes remafin to be fully elucidated. In the present study, alterations in the plasma levels and tissue contents of DNP and the properties of its receptor in diabetic rats, induced by streptozotocin (STZ) injection, were investigated. The plasma levels of DNP were 90.01 ± 4.12 and 196.68 ± 5.60 pg/ml in the control and STZ-induced diabetic rats, respectively. The tissue contents of DNP in the cardiac atrium, ventricle, renal cortex and inner medulla of the STZ-induced diabetic rats were also significantly increased compared with the control rats. Specific (125)I-DNP-binding sites were located predominantly in the glomeruli and inner medulla of the rat kidney. In the glomeruli of the kidney, the apparent dissociation constants (Kd) of (125)I-DNP in the control and STZ-induced diabetic rats were 0.41 ± 0.03 and 0.56 ± 0.06 nM, respectively. The maximum binding capacities (Bmax) of (125)I-DNP in control and STZ-induced diabetic rats were 2.98 ± 0.21 and 6.22 ± 1.06 fmol/mg protein, respectively. However, no differences were observed in the apparent Kd and Bmax of (125)I-DNP in the inner medulla of the kidney between the control and STZ-induced diabetic rats. In the glomerular and inner medullary kidney membranes, DNP stimulated the production of cyclic guanosine monophosphate (cGMP) in a dose-dependent manner. The magnitude of cGMP production in glomerular membranes was greater in the STZ-induced diabetic rats, whereas the magnitude of cGMP production in the inner medullary membranes was lower in the STZ-induced diabetic rats compared with the control rats. These results indicated that STZ-induced diabetes modulate DNP and its receptor, and also suggested that modulation of the DNP system is involved in the renal function of diabetic animals via the intracellular domain of the kidney NP receptor.

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Modified natriuretic peptides and their potential roles in cancer treatment

Liu

Yang

et al. 2022

Biomedical Journal

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Presence of dendroaspis natriuretic peptide and its binding to NPR-A receptor in rabbit kidney

Cited by 5 publications

References 26 publications

Dendroaspis natriuretic peptide is degraded by a metalloproteinase in the rat kidney

Dendroaspis natriuretic peptide is degraded by a metalloproteinase in the rat kidney

Characteristics of dendroaspis natriuretic peptide and its receptor in streptozotocin-induced diabetic rats

Modified natriuretic peptides and their potential roles in cancer treatment

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