Presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle

Tischer, Ilse; Bode, Liv; Apodaca, J; Timm, H.; Peters, D.; Rasch, Ruth; Pociuli, S.; Gerike, E

doi:10.1007/bf01322669

Cited by 77 publications

(64 citation statements)

References 9 publications

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“…Antibodies to PCV1 have been previously demonstrated in mice [21]. Twentyfour mice were euthanised and bled before the starting of the experiment to be sure that the ICR-CD1 mice strain was free of antibodies to PCV1 and PCV2.…”

Section: Discussionmentioning

confidence: 99%

“…Porcine circovirus type 1 (PCV1) serological studies in non-porcine species have also led to controversial results. Tischer et al [21] detected antibodies to PCV1 in human beings, cattle and mice, while other workers did not find any evidence of PCV1 infection in other species (sheep, cattle, turkeys, chickens, human beings, mice, rabbits, goats and ducks) but swine [1]; PCV1 has also been demonstrated as non-pathogenic for swine [2,20]. However, at present, no association has been established between infection of both porcine circoviruses and non-porcine species.…”

Section: Introductionmentioning

confidence: 92%

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Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Quintana

Balasch²,

Segalés

et al. 2002

Vet. Res.

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-The objective of this work was to investigate the susceptibility of rabbits and mice experimentally inoculated with porcine circoviruses type 1 (PCV1) and type 2 (PCV2) to infection and development of disease and/or lesions. Forty six New Zealand rabbits and 50 ICR-CD1 mice were both divided into two groups comprising PCV1 and PCV2 inoculated animals, and a third group inoculated with non-infected cell culture medium. Rabbits were inoculated intranasally while mice were inoculated intraperitoneally. Clinical signs and body weights were recorded at the start of the experiment and at necropsy. Animals were bled, euthanised and necropsied at days 0, 3, 7, 10, 14 and 20 post-inoculation and samples were collected for histopathological, serological, in situ hybridisation and PCR analysis. No clinical signs or gross and microscopic lesions compatible with PCV2 infections such as those seen in pigs were observed. No presence of PCV2 nucleic acid was detected in rabbits and mice by in situ hybridisation. Only one mouse inoculated with PCV1 seroconverted on day 20 PI. PCV1 and PCV2 genome was detected in serum by PCR in mice inoculated with each porcine circovirus, while rabbits were negative for both viral types. These studies indicated that porcine circoviruses did not cause any disease or microscopic lesions in inoculated rabbits and mice during the experimental period. However, intraperitoneally inoculated mice might have harboured PCV2 in circulation without evidence of viral replication. chacun divisés en deux groupes comprenant des animaux inoculés avec PCV1 et PCV2, et un troisième groupe inoculé avec un milieu de culture non-infecté. Les lapins ont été inoculés par voie intranasale et les souris par voie intrapéritonéale. Les signes cliniques et le poids des animaux ont été enregistrés au début de l'expérience et au moment de l'autopsie. Aux jours 0, 3, 7, 10, 14 et 20 après inoculation, le sang des animaux a été prélevé, puis les animaux ont été euthanasiés et ont subi une autopsie ; des échantillons ont été prélevés pour des analyses histopathologique, sérologique, d'hybridation in situ et de PCR. Aucun signe clinique ni lésion macro-ou microscopique correspondant à ceux observés chez les porcs infectés par PCV2 n'ont été détectés. Aucune présence d'acide nucléique de PCV2 n'a été détectée chez les lapins et les souris par hybridation in situ. Seule une souris inoculée par PCV1 est devenue séropositive au jour 20 post-inoculation. Les génomes de PCV1 et PCV2 ont été détectés par PCR dans le sérum de souris inoculées par chacun des circovirus, alors que les lapins sont restés négatifs pour chacun des types. Ces études indiquent que les circovirus porcins n'ont provoqué aucune maladie ou lésion microscopique chez les lapins et les souris inoculés durant toute la durée de l'expérimentation. Cependant, les souris inoculées expérimentalement pourraient avoir hébergé du virus PCV2 dans la circulation sanguine sans signe de réplication virale.lapin / souris / circovirus porcin / réaction en chaîne par polym...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice

Quintana

Balasch²,

Segalés

et al. 2002

Vet. Res.

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“…Na Alemanha, foram detectados por ELISA e por imunofluorescência indireta baixos níveis de anticorpos anti-PCV no soro de 12-69% de camundongos e no de 35% de bovinos (Tischer et al 1995b). O contato com suínos infectados, instalações, equipamentos e fômites são fatores prováveis na transmissão horizontal do ví-rus; fatores de risco causadores de estresse, como densidade elevada, baixa qualidade do ar, água e ração, mistura de lotes com procedência e idades diferentes e presença de enfermidades concomitantes podem intensificar as manifestações clínicas e favorecer o desenvolvimento da doença (Madec et al 2000).…”

Section: Resul II Resul Ii Resul Ii Resul Ii Results T T T Tadunclassified

Circovirose suína

França

Ribeiro²,

Cunha³

et al. 2005

Pesq. Vet. Bras.

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Por meio de revisão da literatura pertinente foram coligidos e são apresentados os principais dados relativos aos aspectos epidemiológicos, clínicos, anátomo e histopatológicos observados na infecção por Circovírus Porcino tipo 2 (PCV-2) em suínos. São abordados a Síndrome Definhante Multissistêmica dos Suínos Desmamados (SDMDS), o Tremor Congênito Suíno (TCS), a Síndrome da Nefropatia e Dermatite Porcina (SNDP), bem como outras enfermidades associadas ou correlatas, a Síndrome Respiratória e Reprodutiva Porcina (SRRP), a Pneumonia Necrotizante Proliferativa (PNP) e as falhas reprodutivas. Uma vez que a SDMSD já foi registrada na Região Sul do Brasil e no Estado do Rio de Janeiro esse estudo objetiva chamar a atenção para o especial significado dessa virose para a suinocultura brasileira, em função dos prejuízos econômicos por ela determinados.

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“…Anelloviruses are small DNA viruses that replicate within the nuclei of infected cells, and CPE due to their presence have not been well described at present. Porcine circoviruses 1 and 2 (PCV1 and PCV2) have also been detected in cell lines including those used for vaccines, and have been traced to the use of porcine trypsin [61][62][63].…”

Section: Adventitious Viruses In Cell-linesmentioning

confidence: 99%