Presence of a centromeric filament during meiosis

Solari, Alberto J.; Tandler, C. J.

doi:10.1139/g91-136

Cited by 13 publications

(4 citation statements)

References 17 publications

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“…We have revealed this domain by using the Os-PPD cytochemical technique by electron microscopy. Interestingly, a similar T-shaped structure called the 'centromeric filament' was previously demonstrated by silver staining in sections of metaphase I centromeres of different vertebrates Solari and Tandler, 1991).…”

Section: The Inner Domain Of Metaphase I Centromeressupporting

confidence: 53%

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

Parra

Viera

Gómez

et al. 2004

Journal of Cell Science

152

179

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SCP3 is a meiosis-specific structural protein appearing at axial elements and lateral elements of the synaptonemal complex. We have analysed the behaviour of SCP3 and the cohesin subunit Rad21 in mouse spermatocytes by means of a squashing technique. Our results demonstrate that both proteins colocalize and are partially released from chromosome arms during late prophase I stages, although they persist at the interchromatid domain of metaphase I bivalents. Thus, Rad21 cannot be considered a `mitotic'-specific variant, but coexists with Rec8. During late prophase I SCP3 and Rad21 accumulate at centromeres, and together with the chromosomal passenger proteins INCENP and aurora-B kinase, show a complex `double cornet'-like distribution at the inner domain of metaphase I centromeres beneath the associated sister kinetochores. We have observed that Rad21 and SCP3 are displaced from centromeres during telophase I when sister kinetochores separate, and are not present at metaphase II centromeres. Thus, we hypothesise that Rad21, and the superimposed SCP3 and SCP2, are involved in the monopolar attachment of sister kinetochores during meiosis I, and are not responsible for the maintenance of sister-chromatid centromere cohesion during meiosis II as previously suggested.

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Section: The Inner Domain Of Metaphase I Centromeressupporting

confidence: 53%

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

Parra

Viera

Gómez

et al. 2004

Journal of Cell Science

152

179

View full text Add to dashboard Cite

show abstract

“…Similar T-shaped signals at the inner domain of metaphase I centromeres have been previously described in mouse spermatocytes by immunofluorescence for the chromosomal passenger proteins INCENP and Aurora-B [ 27 ], the cohesin subunit radiant sensitive protein 21(RAD21), and the LE proteins SYCP3 and SYCP2 [ 28 ]. Likewise, this T-shaped domain has also been detected by electron microscopy by silver staining [ 35 , 36 ] and the Os-PPD cytochemical technique [ 28 ]. At first sight, it seems that all these proteins mostly colocalise at the inner domain of metaphase I centromeres.…”

Section: Discussionmentioning

confidence: 99%

A Perikinetochoric Ring Defined by MCAK and Aurora-B as a Novel Centromere Domain

et al. 2006

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Mitotic Centromere-Associated Kinesin (MCAK) is a member of the kinesin-13 subfamily of kinesin-related proteins. In mitosis, this microtubule-depolymerising kinesin seems to be implicated in chromosome segregation and in the correction of improper kinetochore-microtubule interactions, and its activity is regulated by the Aurora-B kinase. However, there are no published data on its behaviour and function during mammalian meiosis. We have analysed by immunofluorescence in squashed mouse spermatocytes, the distribution and possible function of MCAK, together with Aurora-B, during both meiotic divisions. Our results demonstrate that MCAK and Aurora-B colocalise at the inner domain of metaphase I centromeres. Thus, MCAK shows a “cone”-like three-dimensional distribution beneath and surrounding the closely associated sister kinetochores. During the second meiotic division, MCAK and Aurora-B also colocalise at the inner centromere domain as a band that joins sister kinetochores, but only during prometaphase II in unattached chromosomes. During chromosome congression to the metaphase II plate, MCAK relocalises and appears as a ring below each sister kinetochore. Aurora-B also relocalises to appear as a ring surrounding and beneath kinetochores but during late metaphase II. Our results demonstrate that the redistribution of MCAK at prometaphase II/metaphase II centromeres depends on tension across the centromere and/or on the interaction of microtubules with kinetochores. We propose that the perikinetochoric rings of MCAK and Aurora-B define a novel transient centromere domain at least in mouse chromosomes during meiosis. We discuss the possible functions of MCAK at the inner centromere domain and at the perikinetochoric ring during both meiotic divisions.

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“…Solari (1991) reported evidence from a number of vertebrates that S C lateral element-like structures persist at centromeres and with varying lengths, into chromosome arms until anaphase I and suggested that special staining properties in the centromere region may indicate modified or added components that confer longer persistence there.…”

Section: Discussionmentioning

confidence: 99%

Evidence from a maize desynaptic mutant points to a probable role of synaptonemal complex central region components in provision for subsequent chiasma maintenance

Maguire¹,

Riess²,

Paredes³

1993

Genome

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Homozygotes for the dsy1 desynaptic mutant of maize show massive failure of chiasma maintenance during diplotene and diakinesis. Although some chiasmata persist until anaphase I in most microsporocytes expressing this mutant, homozygotes are completely or nearly completely sterile, owing apparently to disjunctive irregularities. Pachytene synaptic errors and some synaptic failure also are found, but recombination nodules are common in homologously synapsed regions, and equational separation of a heterozygous knob into univalents or open arms at diakinesis clearly demonstrates that chiasma failure occurs following crossing-over. A wider than normal synaptonemal complex central region and uniform apparent weakness of central region cross connections to spreading procedures strongly suggest the presence of a genetic lesion in a synaptonemal complex central region component. The dsy1 mutant may provide an especially important source of material for molecular studies on the nature of chiasma maintenance mechanism.

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Presence of a centromeric filament during meiosis

Cited by 13 publications

References 17 publications

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

A Perikinetochoric Ring Defined by MCAK and Aurora-B as a Novel Centromere Domain

Evidence from a maize desynaptic mutant points to a probable role of synaptonemal complex central region components in provision for subsequent chiasma maintenance

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