Presence, distribution and steroidogenic effect of the peptides orexin A and receptor 1 for orexins in the testis of the South American camelid Alpaca (Vicugna pacos)

Liguori, Giovanna; Assisi, Loredana; Squillacioti, Caterina; Paino, Salvatore; Mirabella, Nicola; Vittoria, Alfredo

doi:10.1016/j.ygcen.2012.07.020

Cited by 18 publications

(38 citation statements)

References 30 publications

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“…The present work reports the expression of the two counterpart substances, OxB and OX 2 , in the same organ, and thus, the existence of the orexinergic complex in the male gonad of the South American camelid is definitely demonstrated. In particular, the presence of OxB in the alpaca testis tissue is supported by the detection of prepro‐orexin previously demonstrated by us (Liguori et al., ), and in the present study, we demonstrate the presence of the specific receptor, OX 2 .…”

Section: Discussionsupporting

confidence: 91%

“…A previous study from our research group described the presence of OxA and its receptor 1 in the alpaca testis (Liguori et al., ). The present work reports the expression of the two counterpart substances, OxB and OX 2 , in the same organ, and thus, the existence of the orexinergic complex in the male gonad of the South American camelid is definitely demonstrated.…”

Section: Discussionmentioning

confidence: 87%

“…More recently, the orexins and their receptors, beyond referred as the orexinergic complex, were found to be present in the adrenals and in other peripheral organs belonging to the gastrointestinal and genital tract. Particularly, the presence of OxA, prepro‐orexin and OX 1 has been detected in the principal cells of the epididymis and in some testicular cytotypes of the rat and alpaca (Assisi et al., ; Liguori et al., , ; Pavone, Tafuri, Avallone, Staiano, & Vittoria, ; Tafuri et al., , ), in the urethro‐prostatic complex of cattle (Russo et al., ) and in the normal, hyperplastic and carcinomatous prostate of humans (Valiante et al., , ). The expression of OX 1 mRNA has been described in the testis of sheep (Zhang et al., ) and chicken (Ohkubo, Tsukada, & Shamoto, ) and in the seminal vesicles, penis and epididymis of men (Karteris, Chen, & Randeva, ).…”

Section: Introductionmentioning

confidence: 99%

“…The study of the orexinergic complex has never been applied, at least in our knowledge, to the genital tract of non‐conventional ruminants of zootechnical interest. For this reason, we began a research on the presence and role played by the complex in the testis of the camelid alpaca ( Vicugna pacos ) (Liguori et al., ), whose breeding extended in the last years from the lands of origin to many countries in the world. The alpaca is a camelid phylogenetically similar to llama ( Lama glama ) and guanaco ( Lama guanicoe ) native of the Andean regions of South America, from Ecuador to southern Chile.…”

Section: Introductionmentioning

confidence: 99%

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Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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Contents The orexins A (OxA) and B (OxB) are two hypothalamic peptides involved in many physiological functions of the mammalian body. They act through the binding of two G‐coupled receptors named receptor 1 (OX1) and receptor 2 (OX2) for orexins. The first receptor is specific for OxA, while the second binds both the substances with equal affinity. The orexins and the relative receptors have been traced by means of different techniques also at the periphery of the body and particularly in the adrenals, and in gastrointestinal and genital organs. Aim of this work was to investigate the presence of OxB and OX2 by means of immunohistochemistry and Western blotting analysis in the testis of the South American camelid alpaca, a species primarily breed in Chile and Ecuador and recently diffused in Europe where the quality of its wool is particularly appreciated. OxB immunoreactivity (IR) was found in the tubular compartment of the testis where spermatogonia (resting), zygotene and pachytene spermatocytes, and spermatids clearly showed differently sized and shaped cytoplasmic positive structures. OX2‐IR was found both in the interstitial and tubular compartments of the testis and particularly in Leydig cells and round and elongated spermatids. Western blotting analysis of testis lysates showed the presence of a protein band whose molecular weight corresponded to that currently assigned to OX2. Such findings easily translate the hypothesis that OxB and its receptor 2 play a functional role both in the interstitial and tubular compartments of the alpaca testis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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“…In addition, it has been demonstrated that GnRH directly up-regulates testosterone production in Leydig cells with a postreceptorial mechanism that involves phospholipase C, COX1 and PGF2 (Zerani et al, 2011). Recently, moreover, it has been postulated that orexin and its receptor 1 are involved in the regulation of steroidogenesis in the alpaca testis (Liguori et al, 2012).…”

Section: Innervation and Immunohistochemical Characteristics Of Epidimentioning

confidence: 99%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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Alpacas (Vicugna pacos) are domesticated camelids indigenous to south America and recently also bred in Europe and Italy for their high quality wool. There is little data available regarding the innervation of the male reproductive tract of this species. In the present study, the distribution of protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), tyrosine hydroxilase (TH), calcitonin gene related peptide (CGRP) and substance P (SP) was analyzed in the epididymis by using immunohistochemical methods. Specimens of the caput, corpus and cauda epididymis were fixed in Bouin's fluid and processed for immunohistochemistry analysis with primary antibodies against PGP 9.5, NPY, TH, CGRP and SP. Immunopositivity to PGP 9.5 and TH and NPY was observed in nerve fibre bundles and in single nerve fibres contained into the peritubular connective tissue. Many TH and NPY immunopositive cells were found to innervate blood vessels. Rare CGRP and SP immunopositive nerves were observed. Several PGP 9,5 and NPY immunopositive epithelial cells were observed in the caput epididymis. The results of the present study suggest a role for the innervations in modulate reproductive functions in the alpaca epididymis.

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The effects of orexin‐A and orexin receptors on anxiety‐ and depression‐related behaviors in a male rat model of post‐traumatic stress disorder

Han

Shi

Han

2021

J of Comparative Neurology

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Orexin neurons play an important role in stress-related mental disorders including post-traumatic stress disorder (PTSD). Anxiety-and depression-related symptoms commonly occur in combination with PTSD. However, the role of the orexin system in mediating alterations in these affective symptoms remains unclear. The medial prefrontal cortex (mPFC) is implicated in both cognitive and emotional processing. In the present study, we investigated anxiety-and depression-related behavioral changes using the elevated plus maze, the sucrose preference test, and the open field test in male rats with single prolonged stress (SPS) induced-PTSD. The expression of orexin-A in the hypothalamus and orexin receptors (OX1R and OX2R) in the mPFC was detected and quantified by immunohistochemistry, western blotting, and realtime polymerase chain reaction. We found that the SPS rats exhibited enhanced levels of anxiety, reduced exploratory activities, and anhedonia. Furthermore, SPS resulted in reductions in the expression of orexin-A in the hypothalamus and the increased the expression of OX1R in the mPFC. The intracerebroventricular administration of orexin-A alleviated behavioral changes in SPS rats and partly restored the increased levels of OX1R in the mPFC. These results suggest that the orexin system plays a role in the anxiety-and depression-related symptoms observed in PTSD.

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Presence, distribution and steroidogenic effect of the peptides orexin A and receptor 1 for orexins in the testis of the South American camelid Alpaca (Vicugna pacos)

Cited by 18 publications

References 30 publications

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

The effects of orexin‐A and orexin receptors on anxiety‐ and depression‐related behaviors in a male rat model of post‐traumatic stress disorder

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